Microsporidia detection and genotyping study of human pathogenic E. bieneusi in animals from Spain.

Microsporidia are ubiquitous parasites infecting all animal phyla and we present evidence that supports their zoonotic potential. Fecal samples taken from domestic (cats and dogs), farm (pigs, rabbits and ostriches) and wild animals (foxes) from different provinces of Spain were evaluated for micros...

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Autores principales:	Ana Luz Galván-Díaz, Angela Magnet, Soledad Fenoy, Nuno Henriques-Gil, María Haro, Francisco Ponce Gordo, Javier Millán, Guadalupe Miró, Carmen del Águila, Fernando Izquierdo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/595b38a04e0742fca63cac026df5abc1
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id	oai:doaj.org-article:595b38a04e0742fca63cac026df5abc1
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spelling	oai:doaj.org-article:595b38a04e0742fca63cac026df5abc12021-11-18T08:27:06ZMicrosporidia detection and genotyping study of human pathogenic E. bieneusi in animals from Spain.1932-620310.1371/journal.pone.0092289https://doaj.org/article/595b38a04e0742fca63cac026df5abc12014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24651457/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Microsporidia are ubiquitous parasites infecting all animal phyla and we present evidence that supports their zoonotic potential. Fecal samples taken from domestic (cats and dogs), farm (pigs, rabbits and ostriches) and wild animals (foxes) from different provinces of Spain were evaluated for microsporidia infection by light microscopy and PCR. After Microsporidia species identification, E. bieneusi genotypes were additionally studied by sequence analysis of the ITS region. Eighty-five samples out of 159 exhibited structures that were compatible with microsporidia spores by Webeŕs stain with 37 of them being confirmed by PCR. Microsporidia species identified included E. bieneusi, E. intestinalis and A. algerae. We report the first diagnosis of E. intestinalis and E. bieneusi in ostriches and A. algerae in pigs. We also provide new information on the molecular characterization of E. bieneusi isolates both in rabbits and ostriches. All of the E. bieneusi genotypes identified belonged to the zoonotic group of genotypes (Group I) including genotypes A (dogs), I (pigs), D (rabbits and foxes) and type IV (ostriches). Our results demonstrate that microsporidia are present in domestic, farm and wild animals in Spain, corroborating their potential role as a source of human infection and environmental contamination.Ana Luz Galván-DíazAngela MagnetSoledad FenoyNuno Henriques-GilMaría HaroFrancisco Ponce GordoJavier MillánGuadalupe MiróCarmen del ÁguilaFernando IzquierdoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e92289 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ana Luz Galván-Díaz Angela Magnet Soledad Fenoy Nuno Henriques-Gil María Haro Francisco Ponce Gordo Javier Millán Guadalupe Miró Carmen del Águila Fernando Izquierdo Microsporidia detection and genotyping study of human pathogenic E. bieneusi in animals from Spain.
description	Microsporidia are ubiquitous parasites infecting all animal phyla and we present evidence that supports their zoonotic potential. Fecal samples taken from domestic (cats and dogs), farm (pigs, rabbits and ostriches) and wild animals (foxes) from different provinces of Spain were evaluated for microsporidia infection by light microscopy and PCR. After Microsporidia species identification, E. bieneusi genotypes were additionally studied by sequence analysis of the ITS region. Eighty-five samples out of 159 exhibited structures that were compatible with microsporidia spores by Webeŕs stain with 37 of them being confirmed by PCR. Microsporidia species identified included E. bieneusi, E. intestinalis and A. algerae. We report the first diagnosis of E. intestinalis and E. bieneusi in ostriches and A. algerae in pigs. We also provide new information on the molecular characterization of E. bieneusi isolates both in rabbits and ostriches. All of the E. bieneusi genotypes identified belonged to the zoonotic group of genotypes (Group I) including genotypes A (dogs), I (pigs), D (rabbits and foxes) and type IV (ostriches). Our results demonstrate that microsporidia are present in domestic, farm and wild animals in Spain, corroborating their potential role as a source of human infection and environmental contamination.
format	article
author	Ana Luz Galván-Díaz Angela Magnet Soledad Fenoy Nuno Henriques-Gil María Haro Francisco Ponce Gordo Javier Millán Guadalupe Miró Carmen del Águila Fernando Izquierdo
author_facet	Ana Luz Galván-Díaz Angela Magnet Soledad Fenoy Nuno Henriques-Gil María Haro Francisco Ponce Gordo Javier Millán Guadalupe Miró Carmen del Águila Fernando Izquierdo
author_sort	Ana Luz Galván-Díaz
title	Microsporidia detection and genotyping study of human pathogenic E. bieneusi in animals from Spain.
title_short	Microsporidia detection and genotyping study of human pathogenic E. bieneusi in animals from Spain.
title_full	Microsporidia detection and genotyping study of human pathogenic E. bieneusi in animals from Spain.
title_fullStr	Microsporidia detection and genotyping study of human pathogenic E. bieneusi in animals from Spain.
title_full_unstemmed	Microsporidia detection and genotyping study of human pathogenic E. bieneusi in animals from Spain.
title_sort	microsporidia detection and genotyping study of human pathogenic e. bieneusi in animals from spain.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/595b38a04e0742fca63cac026df5abc1
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Microsporidia detection and genotyping study of human pathogenic E. bieneusi in animals from Spain.

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