An in vitro investigation to understand the synergistic role of MMPs-1 and 9 on articular cartilage biomechanical properties

Abstract Matrix metalloproteinases (MMPs) play a crucial role in enzymatically digesting cartilage extracellular matrix (ECM) components, resulting in degraded cartilage with altered mechanical loading capacity. Overexpression of MMPs is often caused by trauma, physiologic conditions and by disease....

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Autores principales:	Allison Mixon, Andrew Savage, Ahmed Suparno Bahar-Moni, Malek Adouni, Tanvir Faisal
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/59625733aa864725a8913e40ab61bd3c
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spelling	oai:doaj.org-article:59625733aa864725a8913e40ab61bd3c2021-12-02T15:33:11ZAn in vitro investigation to understand the synergistic role of MMPs-1 and 9 on articular cartilage biomechanical properties10.1038/s41598-021-93744-12045-2322https://doaj.org/article/59625733aa864725a8913e40ab61bd3c2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93744-1https://doaj.org/toc/2045-2322Abstract Matrix metalloproteinases (MMPs) play a crucial role in enzymatically digesting cartilage extracellular matrix (ECM) components, resulting in degraded cartilage with altered mechanical loading capacity. Overexpression of MMPs is often caused by trauma, physiologic conditions and by disease. To understand the synergistic impact MMPs have on cartilage biomechanical properties, MMPs from two subfamilies: collagenase (MMP-1) and gelatinase (MMP-9) were investigated in this study. Three different ratios of MMP-1 (c) and MMP-9 (g), c1:g1, c3:g1 and c1:g3 were considered to develop a degradation model. Thirty samples, harvested from bovine femoral condyles, were treated in groups of 10 with one concentration of enzyme mixture. Each sample was tested in a healthy state prior to introducing degradative enzymes to establish a baseline. Samples were subjected to indentation loading up to 20% bulk strain. Both control and treated samples were mechanically and histologically assessed to determine the impact of degradation. Young’s modulus and peak load of the tissue under indentation were compared between the control and degraded cartilage explants. Cartilage degraded with the c3:g1 enzyme concentration resulted in maximum 33% reduction in stiffness and peak load compared to the other two concentrations. The abundance of collagenase is more responsible for cartilage degradation and reduced mechanical integrity.Allison MixonAndrew SavageAhmed Suparno Bahar-MoniMalek AdouniTanvir FaisalNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Allison Mixon Andrew Savage Ahmed Suparno Bahar-Moni Malek Adouni Tanvir Faisal An in vitro investigation to understand the synergistic role of MMPs-1 and 9 on articular cartilage biomechanical properties
description	Abstract Matrix metalloproteinases (MMPs) play a crucial role in enzymatically digesting cartilage extracellular matrix (ECM) components, resulting in degraded cartilage with altered mechanical loading capacity. Overexpression of MMPs is often caused by trauma, physiologic conditions and by disease. To understand the synergistic impact MMPs have on cartilage biomechanical properties, MMPs from two subfamilies: collagenase (MMP-1) and gelatinase (MMP-9) were investigated in this study. Three different ratios of MMP-1 (c) and MMP-9 (g), c1:g1, c3:g1 and c1:g3 were considered to develop a degradation model. Thirty samples, harvested from bovine femoral condyles, were treated in groups of 10 with one concentration of enzyme mixture. Each sample was tested in a healthy state prior to introducing degradative enzymes to establish a baseline. Samples were subjected to indentation loading up to 20% bulk strain. Both control and treated samples were mechanically and histologically assessed to determine the impact of degradation. Young’s modulus and peak load of the tissue under indentation were compared between the control and degraded cartilage explants. Cartilage degraded with the c3:g1 enzyme concentration resulted in maximum 33% reduction in stiffness and peak load compared to the other two concentrations. The abundance of collagenase is more responsible for cartilage degradation and reduced mechanical integrity.
format	article
author	Allison Mixon Andrew Savage Ahmed Suparno Bahar-Moni Malek Adouni Tanvir Faisal
author_facet	Allison Mixon Andrew Savage Ahmed Suparno Bahar-Moni Malek Adouni Tanvir Faisal
author_sort	Allison Mixon
title	An in vitro investigation to understand the synergistic role of MMPs-1 and 9 on articular cartilage biomechanical properties
title_short	An in vitro investigation to understand the synergistic role of MMPs-1 and 9 on articular cartilage biomechanical properties
title_full	An in vitro investigation to understand the synergistic role of MMPs-1 and 9 on articular cartilage biomechanical properties
title_fullStr	An in vitro investigation to understand the synergistic role of MMPs-1 and 9 on articular cartilage biomechanical properties
title_full_unstemmed	An in vitro investigation to understand the synergistic role of MMPs-1 and 9 on articular cartilage biomechanical properties
title_sort	in vitro investigation to understand the synergistic role of mmps-1 and 9 on articular cartilage biomechanical properties
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/59625733aa864725a8913e40ab61bd3c
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An in vitro investigation to understand the synergistic role of MMPs-1 and 9 on articular cartilage biomechanical properties

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