Effect of expansion media and fibronectin coating on growth and chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells

Abstract In the field of regenerative medicine, considerable advances have been made from the technological and biological point of view. However, there are still large gaps to be filled regarding translation and application of mesenchymal stromal cell (MSC)-based therapies into clinical practice. I...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Valentina Basoli, Elena Della Bella, Eva Johanna Kubosch, Mauro Alini, Martin J. Stoddart
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/59635bc93bf348f5be25f77d03c74ac9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:59635bc93bf348f5be25f77d03c74ac9
record_format	dspace
spelling	oai:doaj.org-article:59635bc93bf348f5be25f77d03c74ac92021-12-02T17:45:03ZEffect of expansion media and fibronectin coating on growth and chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells10.1038/s41598-021-92270-42045-2322https://doaj.org/article/59635bc93bf348f5be25f77d03c74ac92021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92270-4https://doaj.org/toc/2045-2322Abstract In the field of regenerative medicine, considerable advances have been made from the technological and biological point of view. However, there are still large gaps to be filled regarding translation and application of mesenchymal stromal cell (MSC)-based therapies into clinical practice. Indeed, variables such as cell type, unpredictable donor variation, and expansion/differentiation methods lead to inconsistencies. Most protocols use bovine serum (FBS) derivatives during MSC expansion. However, the xenogeneic risks associated with FBS limits the use of MSC-based products in clinical practice. Herein we compare a chemically defined, xenogeneic-free commercial growth medium with a conventional medium containing 10% FBS and 5 ng/ml FGF2. Furthermore, the effect of a fibronectin-coated growth surface was investigated. The effect of the different culture conditions on chondrogenic commitment was assessed by analyzing matrix deposition and gene expression of common chondrogenic markers. Chondrogenic differentiation potential was similar between the FBS-containing αMEM and the chemically defined medium with fibronectin coating. On the contrary, the use of fibronectin coating with FBS-containing medium appeared to reduce the differentiation potential of MSCs. Moreover, cells that were poorly responsive to in vitro chondrogenic stimuli were shown to improve their differentiation potential after expansion in a TGF-β1 containing medium. In conclusion, the use of a xenogeneic-free medium provides a suitable alternative for human bone marrow MSC expansion, due the capability to maintain cell characteristic and potency. To further improve chondrogenic potential of BMSCs, priming the cells with TGF-β1 during expansion is a promising strategy.Valentina BasoliElena Della BellaEva Johanna KuboschMauro AliniMartin J. StoddartNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Valentina Basoli Elena Della Bella Eva Johanna Kubosch Mauro Alini Martin J. Stoddart Effect of expansion media and fibronectin coating on growth and chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells
description	Abstract In the field of regenerative medicine, considerable advances have been made from the technological and biological point of view. However, there are still large gaps to be filled regarding translation and application of mesenchymal stromal cell (MSC)-based therapies into clinical practice. Indeed, variables such as cell type, unpredictable donor variation, and expansion/differentiation methods lead to inconsistencies. Most protocols use bovine serum (FBS) derivatives during MSC expansion. However, the xenogeneic risks associated with FBS limits the use of MSC-based products in clinical practice. Herein we compare a chemically defined, xenogeneic-free commercial growth medium with a conventional medium containing 10% FBS and 5 ng/ml FGF2. Furthermore, the effect of a fibronectin-coated growth surface was investigated. The effect of the different culture conditions on chondrogenic commitment was assessed by analyzing matrix deposition and gene expression of common chondrogenic markers. Chondrogenic differentiation potential was similar between the FBS-containing αMEM and the chemically defined medium with fibronectin coating. On the contrary, the use of fibronectin coating with FBS-containing medium appeared to reduce the differentiation potential of MSCs. Moreover, cells that were poorly responsive to in vitro chondrogenic stimuli were shown to improve their differentiation potential after expansion in a TGF-β1 containing medium. In conclusion, the use of a xenogeneic-free medium provides a suitable alternative for human bone marrow MSC expansion, due the capability to maintain cell characteristic and potency. To further improve chondrogenic potential of BMSCs, priming the cells with TGF-β1 during expansion is a promising strategy.
format	article
author	Valentina Basoli Elena Della Bella Eva Johanna Kubosch Mauro Alini Martin J. Stoddart
author_facet	Valentina Basoli Elena Della Bella Eva Johanna Kubosch Mauro Alini Martin J. Stoddart
author_sort	Valentina Basoli
title	Effect of expansion media and fibronectin coating on growth and chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells
title_short	Effect of expansion media and fibronectin coating on growth and chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells
title_full	Effect of expansion media and fibronectin coating on growth and chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells
title_fullStr	Effect of expansion media and fibronectin coating on growth and chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells
title_full_unstemmed	Effect of expansion media and fibronectin coating on growth and chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells
title_sort	effect of expansion media and fibronectin coating on growth and chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/59635bc93bf348f5be25f77d03c74ac9
work_keys_str_mv	AT valentinabasoli effectofexpansionmediaandfibronectincoatingongrowthandchondrogenicdifferentiationofhumanbonemarrowderivedmesenchymalstromalcells AT elenadellabella effectofexpansionmediaandfibronectincoatingongrowthandchondrogenicdifferentiationofhumanbonemarrowderivedmesenchymalstromalcells AT evajohannakubosch effectofexpansionmediaandfibronectincoatingongrowthandchondrogenicdifferentiationofhumanbonemarrowderivedmesenchymalstromalcells AT mauroalini effectofexpansionmediaandfibronectincoatingongrowthandchondrogenicdifferentiationofhumanbonemarrowderivedmesenchymalstromalcells AT martinjstoddart effectofexpansionmediaandfibronectincoatingongrowthandchondrogenicdifferentiationofhumanbonemarrowderivedmesenchymalstromalcells
_version_	1718379587943006208

Effect of expansion media and fibronectin coating on growth and chondrogenic differentiation of human bone marrow-derived mesenchymal stromal cells

Ejemplares similares