Nrf2 participates in mechanisms for reducing the toxicity and enhancing the antitumour effect of Radix Tripterygium wilfordii to S180-bearing mice by herbal-processing technology

Context: Radix Tripterygium wilfordii Hook. f. (Celastraceae) (LGT) has outstanding curative efficacy; however, side effects include high toxicity, particularly hepatotoxicity and nephrotoxicity. Objective: To investigate detoxification mechanisms of LGT through processing separately with each of th...

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Autores principales:	Jun-Ming Wang, Jin-Yang Li, Hong Cai, Rong-Xing Chen, Yue-Yue Zhang, Lu-Lu Zhang, Ying Cui, Yong-Xian Cheng
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	lonicera japonica thunb lysimachia christinae hance glycyrrhiza uralensis fisch paeonia lactiflora pall phaseolus radiatus l Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/59684b64a4fe4e34963c854d31ac364a
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spelling	oai:doaj.org-article:59684b64a4fe4e34963c854d31ac364a2021-11-17T14:21:56ZNrf2 participates in mechanisms for reducing the toxicity and enhancing the antitumour effect of Radix Tripterygium wilfordii to S180-bearing mice by herbal-processing technology1388-02091744-511610.1080/13880209.2019.1634106https://doaj.org/article/59684b64a4fe4e34963c854d31ac364a2019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1634106https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: Radix Tripterygium wilfordii Hook. f. (Celastraceae) (LGT) has outstanding curative efficacy; however, side effects include high toxicity, particularly hepatotoxicity and nephrotoxicity. Objective: To investigate detoxification mechanisms of LGT through processing separately with each of these medicinal herbs including Flower Lonicera japonica Thunb. (Caprifoliaceae) (JYH), Radix Paeonia lactiflora Pall. (Ranunculaceae) (BS), Herba Lysimachia christinae Hance (Primulaceae) (JQC), Radix et Rhizoma Glycyrrhiza uralensis Fisch. (Fabaceae) (GC) and Seed Phaseolus radiatus L. (Fabaceae) (LD) in S180-bearing mice by involving nuclear factor (erythroid-derived 2)-like 2 (Nrf2). Materials and methods: LGT raw and processed products were orally administered at 60 mg/kg to KM male mice inoculated with S180 tumour cells for 14 consecutive days, and blood, tumour, liver and kidney were taken to observe the detoxifying effects and biological mechanisms. Results: Herbal-processing technology significantly weakened hepatotoxicity and nephrotoxicity evoked by LGT with ED50 of the converted triptolide in each processed-herb product for serum alanine transaminase, aspartate transaminase, creatinine and urea nitrogen of 9.3, 16.6, 2.5 and 4.2 μg/kg, for liver glutathione, glutathione S-transferase, catalase, tumour necrosis factor-α and interleukin-10 of 114.9, 67.8, 134.1, 7.7, 4171.6 μg/kg, and for kidney 21.9, 20.5, 145.0, 529.7, 19.4 μg/kg, respectively. Moreover, herbal-processing technology promoted the accumulation of Nrf2 into the nucleus, and upregulated mRNA expression of Nrf2 and heme oxygenase-1. Additionally, herbal-processing technology enhanced the tumour inhibition rate with ED50 12.2 μg/kg. Discussion and conclusions: Herbal-processing technology improves the safety and effectiveness of LGT in cancer treatment, and future research may be focused on the Nrf2-related molecules.Jun-Ming WangJin-Yang LiHong CaiRong-Xing ChenYue-Yue ZhangLu-Lu ZhangYing CuiYong-Xian ChengTaylor & Francis Grouparticlelonicera japonica thunblysimachia christinae hanceglycyrrhiza uralensis fischpaeonia lactiflora pallphaseolus radiatus lTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 437-448 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	lonicera japonica thunb lysimachia christinae hance glycyrrhiza uralensis fisch paeonia lactiflora pall phaseolus radiatus l Therapeutics. Pharmacology RM1-950
spellingShingle	lonicera japonica thunb lysimachia christinae hance glycyrrhiza uralensis fisch paeonia lactiflora pall phaseolus radiatus l Therapeutics. Pharmacology RM1-950 Jun-Ming Wang Jin-Yang Li Hong Cai Rong-Xing Chen Yue-Yue Zhang Lu-Lu Zhang Ying Cui Yong-Xian Cheng Nrf2 participates in mechanisms for reducing the toxicity and enhancing the antitumour effect of Radix Tripterygium wilfordii to S180-bearing mice by herbal-processing technology
description	Context: Radix Tripterygium wilfordii Hook. f. (Celastraceae) (LGT) has outstanding curative efficacy; however, side effects include high toxicity, particularly hepatotoxicity and nephrotoxicity. Objective: To investigate detoxification mechanisms of LGT through processing separately with each of these medicinal herbs including Flower Lonicera japonica Thunb. (Caprifoliaceae) (JYH), Radix Paeonia lactiflora Pall. (Ranunculaceae) (BS), Herba Lysimachia christinae Hance (Primulaceae) (JQC), Radix et Rhizoma Glycyrrhiza uralensis Fisch. (Fabaceae) (GC) and Seed Phaseolus radiatus L. (Fabaceae) (LD) in S180-bearing mice by involving nuclear factor (erythroid-derived 2)-like 2 (Nrf2). Materials and methods: LGT raw and processed products were orally administered at 60 mg/kg to KM male mice inoculated with S180 tumour cells for 14 consecutive days, and blood, tumour, liver and kidney were taken to observe the detoxifying effects and biological mechanisms. Results: Herbal-processing technology significantly weakened hepatotoxicity and nephrotoxicity evoked by LGT with ED50 of the converted triptolide in each processed-herb product for serum alanine transaminase, aspartate transaminase, creatinine and urea nitrogen of 9.3, 16.6, 2.5 and 4.2 μg/kg, for liver glutathione, glutathione S-transferase, catalase, tumour necrosis factor-α and interleukin-10 of 114.9, 67.8, 134.1, 7.7, 4171.6 μg/kg, and for kidney 21.9, 20.5, 145.0, 529.7, 19.4 μg/kg, respectively. Moreover, herbal-processing technology promoted the accumulation of Nrf2 into the nucleus, and upregulated mRNA expression of Nrf2 and heme oxygenase-1. Additionally, herbal-processing technology enhanced the tumour inhibition rate with ED50 12.2 μg/kg. Discussion and conclusions: Herbal-processing technology improves the safety and effectiveness of LGT in cancer treatment, and future research may be focused on the Nrf2-related molecules.
format	article
author	Jun-Ming Wang Jin-Yang Li Hong Cai Rong-Xing Chen Yue-Yue Zhang Lu-Lu Zhang Ying Cui Yong-Xian Cheng
author_facet	Jun-Ming Wang Jin-Yang Li Hong Cai Rong-Xing Chen Yue-Yue Zhang Lu-Lu Zhang Ying Cui Yong-Xian Cheng
author_sort	Jun-Ming Wang
title	Nrf2 participates in mechanisms for reducing the toxicity and enhancing the antitumour effect of Radix Tripterygium wilfordii to S180-bearing mice by herbal-processing technology
title_short	Nrf2 participates in mechanisms for reducing the toxicity and enhancing the antitumour effect of Radix Tripterygium wilfordii to S180-bearing mice by herbal-processing technology
title_full	Nrf2 participates in mechanisms for reducing the toxicity and enhancing the antitumour effect of Radix Tripterygium wilfordii to S180-bearing mice by herbal-processing technology
title_fullStr	Nrf2 participates in mechanisms for reducing the toxicity and enhancing the antitumour effect of Radix Tripterygium wilfordii to S180-bearing mice by herbal-processing technology
title_full_unstemmed	Nrf2 participates in mechanisms for reducing the toxicity and enhancing the antitumour effect of Radix Tripterygium wilfordii to S180-bearing mice by herbal-processing technology
title_sort	nrf2 participates in mechanisms for reducing the toxicity and enhancing the antitumour effect of radix tripterygium wilfordii to s180-bearing mice by herbal-processing technology
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/59684b64a4fe4e34963c854d31ac364a
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Nrf2 participates in mechanisms for reducing the toxicity and enhancing the antitumour effect of Radix Tripterygium wilfordii to S180-bearing mice by herbal-processing technology

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