Transcriptome analysis of the effect of diosgenin on autoimmune thyroiditis in a rat model

Abstract In a mouse model of Graves’ disease (GD), diosgenin has been shown to have a therapeutic effect on GD by alleviating goitre. However, research on the effect of diosgenin on autoimmune thyroiditis (AIT) is lacking. In this study, transcriptomics was used to comprehensively analyse the protec...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chengfei Zhang, Lingling Qin, Boju Sun, You Wu, Fengying Zhong, Lili Wu, Tonghua Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/59750dca72844ee8b3393cd8ae75612f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:59750dca72844ee8b3393cd8ae75612f
record_format	dspace
spelling	oai:doaj.org-article:59750dca72844ee8b3393cd8ae75612f2021-12-02T13:17:49ZTranscriptome analysis of the effect of diosgenin on autoimmune thyroiditis in a rat model10.1038/s41598-021-85822-12045-2322https://doaj.org/article/59750dca72844ee8b3393cd8ae75612f2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85822-1https://doaj.org/toc/2045-2322Abstract In a mouse model of Graves’ disease (GD), diosgenin has been shown to have a therapeutic effect on GD by alleviating goitre. However, research on the effect of diosgenin on autoimmune thyroiditis (AIT) is lacking. In this study, transcriptomics was used to comprehensively analyse the protective effect of diosgenin against AIT in rats and the possible mechanism. The results showed that in the diosgenin-intervention group, compared to the model group, the expression of serum triiodothyronine, thyroxine, free triiodothyronine, and free thyroxine was decreased and that of thyroid-stimulating hormone was increased; these changes were accompanied by the downregulation of thyroglobulin, TSH receptor antibody and thyroid peroxidase expression in serum. Furthermore, transcriptome detection, RT-qPCR and immunohistochemistry verification revealed that in thyroid tissue, the relative mRNA and protein expression of cyclic adenosine 3′,5′-monophosphate (cAMP), protein kinase A (PKA) and cAMP response element-binding protein (Creb) were increased and the mRNA expression of S100 calcium-binding protein A9 (S100A9) was decreased in the diosgenin groups. In summary, diosgenin alleviates the development of AIT, possibly via the activation of the cAMP/PKA/Creb pathway and downregulation of S100A9 gene expression.Chengfei ZhangLingling QinBoju SunYou WuFengying ZhongLili WuTonghua LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chengfei Zhang Lingling Qin Boju Sun You Wu Fengying Zhong Lili Wu Tonghua Liu Transcriptome analysis of the effect of diosgenin on autoimmune thyroiditis in a rat model
description	Abstract In a mouse model of Graves’ disease (GD), diosgenin has been shown to have a therapeutic effect on GD by alleviating goitre. However, research on the effect of diosgenin on autoimmune thyroiditis (AIT) is lacking. In this study, transcriptomics was used to comprehensively analyse the protective effect of diosgenin against AIT in rats and the possible mechanism. The results showed that in the diosgenin-intervention group, compared to the model group, the expression of serum triiodothyronine, thyroxine, free triiodothyronine, and free thyroxine was decreased and that of thyroid-stimulating hormone was increased; these changes were accompanied by the downregulation of thyroglobulin, TSH receptor antibody and thyroid peroxidase expression in serum. Furthermore, transcriptome detection, RT-qPCR and immunohistochemistry verification revealed that in thyroid tissue, the relative mRNA and protein expression of cyclic adenosine 3′,5′-monophosphate (cAMP), protein kinase A (PKA) and cAMP response element-binding protein (Creb) were increased and the mRNA expression of S100 calcium-binding protein A9 (S100A9) was decreased in the diosgenin groups. In summary, diosgenin alleviates the development of AIT, possibly via the activation of the cAMP/PKA/Creb pathway and downregulation of S100A9 gene expression.
format	article
author	Chengfei Zhang Lingling Qin Boju Sun You Wu Fengying Zhong Lili Wu Tonghua Liu
author_facet	Chengfei Zhang Lingling Qin Boju Sun You Wu Fengying Zhong Lili Wu Tonghua Liu
author_sort	Chengfei Zhang
title	Transcriptome analysis of the effect of diosgenin on autoimmune thyroiditis in a rat model
title_short	Transcriptome analysis of the effect of diosgenin on autoimmune thyroiditis in a rat model
title_full	Transcriptome analysis of the effect of diosgenin on autoimmune thyroiditis in a rat model
title_fullStr	Transcriptome analysis of the effect of diosgenin on autoimmune thyroiditis in a rat model
title_full_unstemmed	Transcriptome analysis of the effect of diosgenin on autoimmune thyroiditis in a rat model
title_sort	transcriptome analysis of the effect of diosgenin on autoimmune thyroiditis in a rat model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/59750dca72844ee8b3393cd8ae75612f
work_keys_str_mv	AT chengfeizhang transcriptomeanalysisoftheeffectofdiosgeninonautoimmunethyroiditisinaratmodel AT linglingqin transcriptomeanalysisoftheeffectofdiosgeninonautoimmunethyroiditisinaratmodel AT bojusun transcriptomeanalysisoftheeffectofdiosgeninonautoimmunethyroiditisinaratmodel AT youwu transcriptomeanalysisoftheeffectofdiosgeninonautoimmunethyroiditisinaratmodel AT fengyingzhong transcriptomeanalysisoftheeffectofdiosgeninonautoimmunethyroiditisinaratmodel AT liliwu transcriptomeanalysisoftheeffectofdiosgeninonautoimmunethyroiditisinaratmodel AT tonghualiu transcriptomeanalysisoftheeffectofdiosgeninonautoimmunethyroiditisinaratmodel
_version_	1718393332532510720

Transcriptome analysis of the effect of diosgenin on autoimmune thyroiditis in a rat model

Ejemplares similares