Three-channel ion chromatograph for improved metabolic evaluation of urolithiasis

Abstract Background Urolithiasis is a multi-etiological disease resulting from a combination of environmental and genetic factors. One of the most challenging aspects of this disease is its high recurrence rate. For most patients, an in-depth metabolic evaluation may reveal the presence of urinary s...

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Autores principales:	Qiang Li, Guanlin Liu, Yue Cheng, Wenbo Tang
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Citrate Ion chromatograph Metabolic evaluation Oxalate Urinary calculus Urolithiasis Diseases of the genitourinary system. Urology RC870-923
Acceso en línea:	https://doaj.org/article/597dfa7258844cc496aedcd3309093df
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spelling	oai:doaj.org-article:597dfa7258844cc496aedcd3309093df2021-11-08T11:02:45ZThree-channel ion chromatograph for improved metabolic evaluation of urolithiasis10.1186/s12894-021-00914-41471-2490https://doaj.org/article/597dfa7258844cc496aedcd3309093df2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12894-021-00914-4https://doaj.org/toc/1471-2490Abstract Background Urolithiasis is a multi-etiological disease resulting from a combination of environmental and genetic factors. One of the most challenging aspects of this disease is its high recurrence rate. For most patients, an in-depth metabolic evaluation may reveal the presence of urinary stones. The fact that different urinary stone-related compounds (USRCs) are measured by different methods renders the metabolic evaluation of urolithiasis quite tedious and complex. Methods A three-channel ion chromatograph (IC) that automatically measures the concentration of common metabolic indicators of urolithiasis in urine (i.e., oxalate, citrate, uric acid, calcium, and magnesium) was developed to improve the efficiency. To validate its precision and specificity, standard curves were prepared using working solution of these indicators. 100 standard solutions of these indicators were measured with our new IC and three other ICs as the control instruments; analyte concentrations in 100 24-h urine samples from volunteers and 135 calculi patients were also measured. Results All analytes had good linear relationships in concentration ranges of 0–10 mg/L. The precision experiments in the standard and urine samples showed that the measurement errors of the newly developed IC were all less than 5%. In urine, the recovery rate ranged from 99.6 to 100.4%, the coefficient of variation ranged from 1.39 to 2.99%, and the results matched between our newly developed IC and the control ICs. The results of the efficiency test showed that we can finish the analysis at the average number of 14 people per day with the new IC. While the average number in the control group is 3.85/day (p = 0.000). Conclusions Overall, this multi-channel system significantly improves the efficiency of metabolic evaluation while retaining accuracy and precision.Qiang LiGuanlin LiuYue ChengWenbo TangBMCarticleCitrateIon chromatographMetabolic evaluationOxalateUrinary calculusUrolithiasisDiseases of the genitourinary system. UrologyRC870-923ENBMC Urology, Vol 21, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Citrate Ion chromatograph Metabolic evaluation Oxalate Urinary calculus Urolithiasis Diseases of the genitourinary system. Urology RC870-923
spellingShingle	Citrate Ion chromatograph Metabolic evaluation Oxalate Urinary calculus Urolithiasis Diseases of the genitourinary system. Urology RC870-923 Qiang Li Guanlin Liu Yue Cheng Wenbo Tang Three-channel ion chromatograph for improved metabolic evaluation of urolithiasis
description	Abstract Background Urolithiasis is a multi-etiological disease resulting from a combination of environmental and genetic factors. One of the most challenging aspects of this disease is its high recurrence rate. For most patients, an in-depth metabolic evaluation may reveal the presence of urinary stones. The fact that different urinary stone-related compounds (USRCs) are measured by different methods renders the metabolic evaluation of urolithiasis quite tedious and complex. Methods A three-channel ion chromatograph (IC) that automatically measures the concentration of common metabolic indicators of urolithiasis in urine (i.e., oxalate, citrate, uric acid, calcium, and magnesium) was developed to improve the efficiency. To validate its precision and specificity, standard curves were prepared using working solution of these indicators. 100 standard solutions of these indicators were measured with our new IC and three other ICs as the control instruments; analyte concentrations in 100 24-h urine samples from volunteers and 135 calculi patients were also measured. Results All analytes had good linear relationships in concentration ranges of 0–10 mg/L. The precision experiments in the standard and urine samples showed that the measurement errors of the newly developed IC were all less than 5%. In urine, the recovery rate ranged from 99.6 to 100.4%, the coefficient of variation ranged from 1.39 to 2.99%, and the results matched between our newly developed IC and the control ICs. The results of the efficiency test showed that we can finish the analysis at the average number of 14 people per day with the new IC. While the average number in the control group is 3.85/day (p = 0.000). Conclusions Overall, this multi-channel system significantly improves the efficiency of metabolic evaluation while retaining accuracy and precision.
format	article
author	Qiang Li Guanlin Liu Yue Cheng Wenbo Tang
author_facet	Qiang Li Guanlin Liu Yue Cheng Wenbo Tang
author_sort	Qiang Li
title	Three-channel ion chromatograph for improved metabolic evaluation of urolithiasis
title_short	Three-channel ion chromatograph for improved metabolic evaluation of urolithiasis
title_full	Three-channel ion chromatograph for improved metabolic evaluation of urolithiasis
title_fullStr	Three-channel ion chromatograph for improved metabolic evaluation of urolithiasis
title_full_unstemmed	Three-channel ion chromatograph for improved metabolic evaluation of urolithiasis
title_sort	three-channel ion chromatograph for improved metabolic evaluation of urolithiasis
publisher	BMC
publishDate	2021
url	https://doaj.org/article/597dfa7258844cc496aedcd3309093df
work_keys_str_mv	AT qiangli threechannelionchromatographforimprovedmetabolicevaluationofurolithiasis AT guanlinliu threechannelionchromatographforimprovedmetabolicevaluationofurolithiasis AT yuecheng threechannelionchromatographforimprovedmetabolicevaluationofurolithiasis AT wenbotang threechannelionchromatographforimprovedmetabolicevaluationofurolithiasis
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Three-channel ion chromatograph for improved metabolic evaluation of urolithiasis

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