In vitro apoptotic and DNA damaging potential of nanobarium oxide

Saud Alarifi,1 Daoud Ali,1 Widad Al-Bishri2 1Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia; 2Department of Biochemistry, Faculty of Science-Al Faisaliah Campus, King Abdulaziz University, Jeddah, Saudi Arabia Abstract: Barium oxide nanoparticles (BaONPs) are...

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Autores principales:	Alarifi S, Ali D, Al-Bishri W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Nano-barium oxide L929 cells DNA damage Apoptosis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/59803db405064ea99e3246e1ba157bec
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spelling	oai:doaj.org-article:59803db405064ea99e3246e1ba157bec2021-12-02T01:32:33ZIn vitro apoptotic and DNA damaging potential of nanobarium oxide1178-2013https://doaj.org/article/59803db405064ea99e3246e1ba157bec2016-01-01T00:00:00Zhttps://www.dovepress.com/in-vitro-apoptotic-and-dna-damaging-potential-of-nanobarium-oxide-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Saud Alarifi,1 Daoud Ali,1 Widad Al-Bishri2 1Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia; 2Department of Biochemistry, Faculty of Science-Al Faisaliah Campus, King Abdulaziz University, Jeddah, Saudi Arabia Abstract: Barium oxide nanoparticles (BaONPs) are an important industrial compound and are widely used in polymers and paints. In this study, apoptotic and genotoxic effects of BaONPs in mouse embryonic fibroblast (L929) cells were determined by using single-cell gel test. In vitro cytotoxicity assays were performed to assess BaONPs’ toxicity in L929 cells. Mild cytotoxicity was observed in L929 cells due to BaONPs. BaONPs increased lipid peroxidation, catalase, and superoxide dismutase levels and lowered glutathione levels in L929 cells. This was accompanied by concomitant generation of reactive oxygen species and activation of caspase-3 in BaONPs-treated L929 cells. On the other hand, when we exposed L929 cells to BaONPs for 24 and 48 hours (comet assay), there was a duration- and dose-dependent increase in DNA impairment detected in the single-cell gel test. Thus, BaONPs exhibit genotoxic and apoptotic effects in L929 cells, most likely due to initiation of oxidative damage. Keywords: nanobarium oxide, L929 cells, DNA damage, apoptosisAlarifi SAli DAl-Bishri WDove Medical PressarticleNano-barium oxideL929 cellsDNA damageApoptosisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss Issue 1, Pp 249-257 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nano-barium oxide L929 cells DNA damage Apoptosis Medicine (General) R5-920
spellingShingle	Nano-barium oxide L929 cells DNA damage Apoptosis Medicine (General) R5-920 Alarifi S Ali D Al-Bishri W In vitro apoptotic and DNA damaging potential of nanobarium oxide
description	Saud Alarifi,1 Daoud Ali,1 Widad Al-Bishri2 1Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia; 2Department of Biochemistry, Faculty of Science-Al Faisaliah Campus, King Abdulaziz University, Jeddah, Saudi Arabia Abstract: Barium oxide nanoparticles (BaONPs) are an important industrial compound and are widely used in polymers and paints. In this study, apoptotic and genotoxic effects of BaONPs in mouse embryonic fibroblast (L929) cells were determined by using single-cell gel test. In vitro cytotoxicity assays were performed to assess BaONPs’ toxicity in L929 cells. Mild cytotoxicity was observed in L929 cells due to BaONPs. BaONPs increased lipid peroxidation, catalase, and superoxide dismutase levels and lowered glutathione levels in L929 cells. This was accompanied by concomitant generation of reactive oxygen species and activation of caspase-3 in BaONPs-treated L929 cells. On the other hand, when we exposed L929 cells to BaONPs for 24 and 48 hours (comet assay), there was a duration- and dose-dependent increase in DNA impairment detected in the single-cell gel test. Thus, BaONPs exhibit genotoxic and apoptotic effects in L929 cells, most likely due to initiation of oxidative damage. Keywords: nanobarium oxide, L929 cells, DNA damage, apoptosis
format	article
author	Alarifi S Ali D Al-Bishri W
author_facet	Alarifi S Ali D Al-Bishri W
author_sort	Alarifi S
title	In vitro apoptotic and DNA damaging potential of nanobarium oxide
title_short	In vitro apoptotic and DNA damaging potential of nanobarium oxide
title_full	In vitro apoptotic and DNA damaging potential of nanobarium oxide
title_fullStr	In vitro apoptotic and DNA damaging potential of nanobarium oxide
title_full_unstemmed	In vitro apoptotic and DNA damaging potential of nanobarium oxide
title_sort	in vitro apoptotic and dna damaging potential of nanobarium oxide
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/59803db405064ea99e3246e1ba157bec
work_keys_str_mv	AT alarifis invitroapoptoticanddnadamagingpotentialofnanobariumoxide AT alid invitroapoptoticanddnadamagingpotentialofnanobariumoxide AT albishriw invitroapoptoticanddnadamagingpotentialofnanobariumoxide
_version_	1718403016059518976

In vitro apoptotic and DNA damaging potential of nanobarium oxide

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