Molecular cloning and characterization of a newly isolated pyrethroid-degrading esterase gene from a genomic library of Ochrobactrum anthropi YZ-1.

A novel pyrethroid-degrading esterase gene pytY was isolated from the genomic library of Ochrobactrum anthropi YZ-1. It possesses an open reading frame (ORF) of 897 bp. Blast search showed that its deduced amino acid sequence shares moderate identities (30% to 46%) with most homologous esterases. Ph...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhiyong Ruan, Yi Zhai, Jinlong Song, Yanhua Shi, Kang Li, Bin Zhao, Yanchun Yan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/59899a11990144bd8da882065f040f0e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:59899a11990144bd8da882065f040f0e
record_format	dspace
spelling	oai:doaj.org-article:59899a11990144bd8da882065f040f0e2021-11-18T08:51:12ZMolecular cloning and characterization of a newly isolated pyrethroid-degrading esterase gene from a genomic library of Ochrobactrum anthropi YZ-1.1932-620310.1371/journal.pone.0077329https://doaj.org/article/59899a11990144bd8da882065f040f0e2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24155944/?tool=EBIhttps://doaj.org/toc/1932-6203A novel pyrethroid-degrading esterase gene pytY was isolated from the genomic library of Ochrobactrum anthropi YZ-1. It possesses an open reading frame (ORF) of 897 bp. Blast search showed that its deduced amino acid sequence shares moderate identities (30% to 46%) with most homologous esterases. Phylogenetic analysis revealed that PytY is a member of the esterase VI family. pytY showed very low sequence similarity compared with reported pyrethroid-degrading genes. PytY was expressed, purified, and characterized. Enzyme assay revealed that PytY is a broad-spectrum degrading enzyme that can degrade various pyrethroids. It is a new pyrethroid-degrading gene and enriches genetic resource. Kinetic constants of Km and Vmax were 2.34 mmol·L(-1) and 56.33 nmol min(-1), respectively, with lambda-cyhalothrin as substrate. PytY displayed good degrading ability and stability over a broad range of temperature and pH. The optimal temperature and pH were of 35°C and 7.5. No cofactors were required for enzyme activity. The results highlighted the potential use of PytY in the elimination of pyrethroid residuals from contaminated environments.Zhiyong RuanYi ZhaiJinlong SongYanhua ShiKang LiBin ZhaoYanchun YanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e77329 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhiyong Ruan Yi Zhai Jinlong Song Yanhua Shi Kang Li Bin Zhao Yanchun Yan Molecular cloning and characterization of a newly isolated pyrethroid-degrading esterase gene from a genomic library of Ochrobactrum anthropi YZ-1.
description	A novel pyrethroid-degrading esterase gene pytY was isolated from the genomic library of Ochrobactrum anthropi YZ-1. It possesses an open reading frame (ORF) of 897 bp. Blast search showed that its deduced amino acid sequence shares moderate identities (30% to 46%) with most homologous esterases. Phylogenetic analysis revealed that PytY is a member of the esterase VI family. pytY showed very low sequence similarity compared with reported pyrethroid-degrading genes. PytY was expressed, purified, and characterized. Enzyme assay revealed that PytY is a broad-spectrum degrading enzyme that can degrade various pyrethroids. It is a new pyrethroid-degrading gene and enriches genetic resource. Kinetic constants of Km and Vmax were 2.34 mmol·L(-1) and 56.33 nmol min(-1), respectively, with lambda-cyhalothrin as substrate. PytY displayed good degrading ability and stability over a broad range of temperature and pH. The optimal temperature and pH were of 35°C and 7.5. No cofactors were required for enzyme activity. The results highlighted the potential use of PytY in the elimination of pyrethroid residuals from contaminated environments.
format	article
author	Zhiyong Ruan Yi Zhai Jinlong Song Yanhua Shi Kang Li Bin Zhao Yanchun Yan
author_facet	Zhiyong Ruan Yi Zhai Jinlong Song Yanhua Shi Kang Li Bin Zhao Yanchun Yan
author_sort	Zhiyong Ruan
title	Molecular cloning and characterization of a newly isolated pyrethroid-degrading esterase gene from a genomic library of Ochrobactrum anthropi YZ-1.
title_short	Molecular cloning and characterization of a newly isolated pyrethroid-degrading esterase gene from a genomic library of Ochrobactrum anthropi YZ-1.
title_full	Molecular cloning and characterization of a newly isolated pyrethroid-degrading esterase gene from a genomic library of Ochrobactrum anthropi YZ-1.
title_fullStr	Molecular cloning and characterization of a newly isolated pyrethroid-degrading esterase gene from a genomic library of Ochrobactrum anthropi YZ-1.
title_full_unstemmed	Molecular cloning and characterization of a newly isolated pyrethroid-degrading esterase gene from a genomic library of Ochrobactrum anthropi YZ-1.
title_sort	molecular cloning and characterization of a newly isolated pyrethroid-degrading esterase gene from a genomic library of ochrobactrum anthropi yz-1.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/59899a11990144bd8da882065f040f0e
work_keys_str_mv	AT zhiyongruan molecularcloningandcharacterizationofanewlyisolatedpyrethroiddegradingesterasegenefromagenomiclibraryofochrobactrumanthropiyz1 AT yizhai molecularcloningandcharacterizationofanewlyisolatedpyrethroiddegradingesterasegenefromagenomiclibraryofochrobactrumanthropiyz1 AT jinlongsong molecularcloningandcharacterizationofanewlyisolatedpyrethroiddegradingesterasegenefromagenomiclibraryofochrobactrumanthropiyz1 AT yanhuashi molecularcloningandcharacterizationofanewlyisolatedpyrethroiddegradingesterasegenefromagenomiclibraryofochrobactrumanthropiyz1 AT kangli molecularcloningandcharacterizationofanewlyisolatedpyrethroiddegradingesterasegenefromagenomiclibraryofochrobactrumanthropiyz1 AT binzhao molecularcloningandcharacterizationofanewlyisolatedpyrethroiddegradingesterasegenefromagenomiclibraryofochrobactrumanthropiyz1 AT yanchunyan molecularcloningandcharacterizationofanewlyisolatedpyrethroiddegradingesterasegenefromagenomiclibraryofochrobactrumanthropiyz1
_version_	1718421262786625536

Molecular cloning and characterization of a newly isolated pyrethroid-degrading esterase gene from a genomic library of Ochrobactrum anthropi YZ-1.

Ejemplares similares