Memory immune responses against pandemic (H1N1) 2009 influenza virus induced by a whole particle vaccine in cynomolgus monkeys carrying Mafa-A1*052:02.

We made an H1N1 vaccine candidate from a virus library consisting of 144 ( = 16 HA×9 NA) non-pathogenic influenza A viruses and examined its protective effects against a pandemic (2009) H1N1 strain using immunologically naïve cynomolgus macaques to exclude preexisting immunity and to employ a precli...

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Autores principales:	Masahiko Arikata, Yasushi Itoh, Masatoshi Okamatsu, Toshinaga Maeda, Takashi Shiina, Keiko Tanaka, Shingo Suzuki, Misako Nakayama, Yoshihiro Sakoda, Hirohito Ishigaki, Ayato Takada, Hideaki Ishida, Kosuke Soda, Van Loi Pham, Hideaki Tsuchiya, Shinichiro Nakamura, Ryuzo Torii, Takeshi Shimizu, Hidetoshi Inoko, Iwao Ohkubo, Hiroshi Kida, Kazumasa Ogasawara
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/598b83786227441f893eae280be85bcf
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spelling	oai:doaj.org-article:598b83786227441f893eae280be85bcf2021-11-18T07:18:11ZMemory immune responses against pandemic (H1N1) 2009 influenza virus induced by a whole particle vaccine in cynomolgus monkeys carrying Mafa-A1052:02.1932-620310.1371/journal.pone.0037220https://doaj.org/article/598b83786227441f893eae280be85bcf2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22623997/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203We made an H1N1 vaccine candidate from a virus library consisting of 144 ( = 16 HA×9 NA) non-pathogenic influenza A viruses and examined its protective effects against a pandemic (2009) H1N1 strain using immunologically naïve cynomolgus macaques to exclude preexisting immunity and to employ a preclinical study since preexisting immunity in humans previously vaccinated or infected with influenza virus might make comparison of vaccine efficacy difficult. Furthermore, macaques carrying a major histocompatibility complex class I molecule, Mafa-A1052:02, were used to analyze peptide-specific CD8(+) T cell responses. Sera of macaques immunized with an inactivated whole particle formulation without addition of an adjuvant showed higher neutralization titers against the vaccine strain A/Hokkaido/2/1981 (H1N1) than did sera of macaques immunized with a split formulation. Neutralization activities against the pandemic strain A/Narita/1/2009 (H1N1) in sera of macaques immunized twice with the split vaccine reached levels similar to those in sera of macaques immunized once with the whole particle vaccine. After inoculation with the pandemic virus, the virus was detected in nasal samples of unvaccinated macaques for 6 days after infection and for 2.67 days and 5.33 days on average in macaques vaccinated with the whole particle vaccine and the split vaccine, respectively. After the challenge infection, recall neutralizing antibody responses against the pandemic virus and CD8(+) T cell responses specific for nucleoprotein peptide NP262-270 bound to Mafa-A1*052:02 in macaques vaccinated with the whole particle vaccine were observed more promptly or more vigorously than those in macaques vaccinated with the split vaccine. These findings demonstrated that the vaccine derived from our virus library was effective for pandemic virus infection in macaques and that the whole particle vaccine conferred more effective memory and broader cross-reactive immune responses to macaques against pandemic influenza virus infection than did the split vaccine.Masahiko ArikataYasushi ItohMasatoshi OkamatsuToshinaga MaedaTakashi ShiinaKeiko TanakaShingo SuzukiMisako NakayamaYoshihiro SakodaHirohito IshigakiAyato TakadaHideaki IshidaKosuke SodaVan Loi PhamHideaki TsuchiyaShinichiro NakamuraRyuzo ToriiTakeshi ShimizuHidetoshi InokoIwao OhkuboHiroshi KidaKazumasa OgasawaraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e37220 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Masahiko Arikata Yasushi Itoh Masatoshi Okamatsu Toshinaga Maeda Takashi Shiina Keiko Tanaka Shingo Suzuki Misako Nakayama Yoshihiro Sakoda Hirohito Ishigaki Ayato Takada Hideaki Ishida Kosuke Soda Van Loi Pham Hideaki Tsuchiya Shinichiro Nakamura Ryuzo Torii Takeshi Shimizu Hidetoshi Inoko Iwao Ohkubo Hiroshi Kida Kazumasa Ogasawara Memory immune responses against pandemic (H1N1) 2009 influenza virus induced by a whole particle vaccine in cynomolgus monkeys carrying Mafa-A1*052:02.
description	We made an H1N1 vaccine candidate from a virus library consisting of 144 ( = 16 HA×9 NA) non-pathogenic influenza A viruses and examined its protective effects against a pandemic (2009) H1N1 strain using immunologically naïve cynomolgus macaques to exclude preexisting immunity and to employ a preclinical study since preexisting immunity in humans previously vaccinated or infected with influenza virus might make comparison of vaccine efficacy difficult. Furthermore, macaques carrying a major histocompatibility complex class I molecule, Mafa-A1052:02, were used to analyze peptide-specific CD8(+) T cell responses. Sera of macaques immunized with an inactivated whole particle formulation without addition of an adjuvant showed higher neutralization titers against the vaccine strain A/Hokkaido/2/1981 (H1N1) than did sera of macaques immunized with a split formulation. Neutralization activities against the pandemic strain A/Narita/1/2009 (H1N1) in sera of macaques immunized twice with the split vaccine reached levels similar to those in sera of macaques immunized once with the whole particle vaccine. After inoculation with the pandemic virus, the virus was detected in nasal samples of unvaccinated macaques for 6 days after infection and for 2.67 days and 5.33 days on average in macaques vaccinated with the whole particle vaccine and the split vaccine, respectively. After the challenge infection, recall neutralizing antibody responses against the pandemic virus and CD8(+) T cell responses specific for nucleoprotein peptide NP262-270 bound to Mafa-A1052:02 in macaques vaccinated with the whole particle vaccine were observed more promptly or more vigorously than those in macaques vaccinated with the split vaccine. These findings demonstrated that the vaccine derived from our virus library was effective for pandemic virus infection in macaques and that the whole particle vaccine conferred more effective memory and broader cross-reactive immune responses to macaques against pandemic influenza virus infection than did the split vaccine.
format	article
author	Masahiko Arikata Yasushi Itoh Masatoshi Okamatsu Toshinaga Maeda Takashi Shiina Keiko Tanaka Shingo Suzuki Misako Nakayama Yoshihiro Sakoda Hirohito Ishigaki Ayato Takada Hideaki Ishida Kosuke Soda Van Loi Pham Hideaki Tsuchiya Shinichiro Nakamura Ryuzo Torii Takeshi Shimizu Hidetoshi Inoko Iwao Ohkubo Hiroshi Kida Kazumasa Ogasawara
author_facet	Masahiko Arikata Yasushi Itoh Masatoshi Okamatsu Toshinaga Maeda Takashi Shiina Keiko Tanaka Shingo Suzuki Misako Nakayama Yoshihiro Sakoda Hirohito Ishigaki Ayato Takada Hideaki Ishida Kosuke Soda Van Loi Pham Hideaki Tsuchiya Shinichiro Nakamura Ryuzo Torii Takeshi Shimizu Hidetoshi Inoko Iwao Ohkubo Hiroshi Kida Kazumasa Ogasawara
author_sort	Masahiko Arikata
title	Memory immune responses against pandemic (H1N1) 2009 influenza virus induced by a whole particle vaccine in cynomolgus monkeys carrying Mafa-A1*052:02.
title_short	Memory immune responses against pandemic (H1N1) 2009 influenza virus induced by a whole particle vaccine in cynomolgus monkeys carrying Mafa-A1*052:02.
title_full	Memory immune responses against pandemic (H1N1) 2009 influenza virus induced by a whole particle vaccine in cynomolgus monkeys carrying Mafa-A1*052:02.
title_fullStr	Memory immune responses against pandemic (H1N1) 2009 influenza virus induced by a whole particle vaccine in cynomolgus monkeys carrying Mafa-A1*052:02.
title_full_unstemmed	Memory immune responses against pandemic (H1N1) 2009 influenza virus induced by a whole particle vaccine in cynomolgus monkeys carrying Mafa-A1*052:02.
title_sort	memory immune responses against pandemic (h1n1) 2009 influenza virus induced by a whole particle vaccine in cynomolgus monkeys carrying mafa-a1*052:02.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/598b83786227441f893eae280be85bcf
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Memory immune responses against pandemic (H1N1) 2009 influenza virus induced by a whole particle vaccine in cynomolgus monkeys carrying Mafa-A1*052:02.

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