Effects of Vitamin D3 and Meso-Zeaxanthin on Human Retinal Pigmented Epithelial Cells in Three Integrated in vitro Paradigms of Age-Related Macular Degeneration

Age-related macular degeneration (AMD) is a degenerative retinal disease and one of major causes of irreversible vision loss. AMD has been linked to several pathological factors, such as oxidative stress and inflammation. Moreover, Aβ (1–42) oligomers have been found in drusen, the extracellular dep...

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Autores principales:	Francesca Lazzara, Federica Conti, Chiara Bianca Maria Platania, Chiara M. Eandi, Filippo Drago, Claudio Bucolo
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	1,25(OH)2D3 meso-zeaxanthin amyloid beta inflammation oxidative stress cytokines Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/5993f80cbbba4e4388f14a9be33dec41
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spelling	oai:doaj.org-article:5993f80cbbba4e4388f14a9be33dec412021-11-05T08:30:11ZEffects of Vitamin D3 and Meso-Zeaxanthin on Human Retinal Pigmented Epithelial Cells in Three Integrated in vitro Paradigms of Age-Related Macular Degeneration1663-981210.3389/fphar.2021.778165https://doaj.org/article/5993f80cbbba4e4388f14a9be33dec412021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2021.778165/fullhttps://doaj.org/toc/1663-9812Age-related macular degeneration (AMD) is a degenerative retinal disease and one of major causes of irreversible vision loss. AMD has been linked to several pathological factors, such as oxidative stress and inflammation. Moreover, Aβ (1–42) oligomers have been found in drusen, the extracellular deposits that accumulate beneath the retinal pigmented epithelium in AMD patients. Hereby, we investigated the hypothesis that treatment with 1,25(OH) 2D3 (vitamin D3) and meso-zeaxathin, physiologically present in the eye, would counteract the toxic effects of three different insults on immortalized human retinal pigmented epithelial cells (ARPE-19). Specifically, ARPE-19 cells have been challenged with Aβ (1–42) oligomers, H2O2, LPS, and TNF-α, respectively. In the present study, we demonstrated that the combination of 1,25(OH)2D3 and meso-zeaxanthin significantly counteracted the cell damage induced by the three insults, at least in these in vitro integrated paradigms of AMD. These results suggest that combination of 1,25(OH)2D3 and meso-zeaxathin could be a useful approach to contrast pathological features of AMD, such as retinal inflammation and oxidative stress.Francesca LazzaraFederica ContiChiara Bianca Maria PlataniaChiara M. EandiChiara M. EandiFilippo DragoFilippo DragoClaudio BucoloClaudio BucoloFrontiers Media S.A.article1,25(OH)2D3meso-zeaxanthinamyloid betainflammationoxidative stresscytokinesTherapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	1,25(OH)2D3 meso-zeaxanthin amyloid beta inflammation oxidative stress cytokines Therapeutics. Pharmacology RM1-950
spellingShingle	1,25(OH)2D3 meso-zeaxanthin amyloid beta inflammation oxidative stress cytokines Therapeutics. Pharmacology RM1-950 Francesca Lazzara Federica Conti Chiara Bianca Maria Platania Chiara M. Eandi Chiara M. Eandi Filippo Drago Filippo Drago Claudio Bucolo Claudio Bucolo Effects of Vitamin D3 and Meso-Zeaxanthin on Human Retinal Pigmented Epithelial Cells in Three Integrated in vitro Paradigms of Age-Related Macular Degeneration
description	Age-related macular degeneration (AMD) is a degenerative retinal disease and one of major causes of irreversible vision loss. AMD has been linked to several pathological factors, such as oxidative stress and inflammation. Moreover, Aβ (1–42) oligomers have been found in drusen, the extracellular deposits that accumulate beneath the retinal pigmented epithelium in AMD patients. Hereby, we investigated the hypothesis that treatment with 1,25(OH) 2D3 (vitamin D3) and meso-zeaxathin, physiologically present in the eye, would counteract the toxic effects of three different insults on immortalized human retinal pigmented epithelial cells (ARPE-19). Specifically, ARPE-19 cells have been challenged with Aβ (1–42) oligomers, H2O2, LPS, and TNF-α, respectively. In the present study, we demonstrated that the combination of 1,25(OH)2D3 and meso-zeaxanthin significantly counteracted the cell damage induced by the three insults, at least in these in vitro integrated paradigms of AMD. These results suggest that combination of 1,25(OH)2D3 and meso-zeaxathin could be a useful approach to contrast pathological features of AMD, such as retinal inflammation and oxidative stress.
format	article
author	Francesca Lazzara Federica Conti Chiara Bianca Maria Platania Chiara M. Eandi Chiara M. Eandi Filippo Drago Filippo Drago Claudio Bucolo Claudio Bucolo
author_facet	Francesca Lazzara Federica Conti Chiara Bianca Maria Platania Chiara M. Eandi Chiara M. Eandi Filippo Drago Filippo Drago Claudio Bucolo Claudio Bucolo
author_sort	Francesca Lazzara
title	Effects of Vitamin D3 and Meso-Zeaxanthin on Human Retinal Pigmented Epithelial Cells in Three Integrated in vitro Paradigms of Age-Related Macular Degeneration
title_short	Effects of Vitamin D3 and Meso-Zeaxanthin on Human Retinal Pigmented Epithelial Cells in Three Integrated in vitro Paradigms of Age-Related Macular Degeneration
title_full	Effects of Vitamin D3 and Meso-Zeaxanthin on Human Retinal Pigmented Epithelial Cells in Three Integrated in vitro Paradigms of Age-Related Macular Degeneration
title_fullStr	Effects of Vitamin D3 and Meso-Zeaxanthin on Human Retinal Pigmented Epithelial Cells in Three Integrated in vitro Paradigms of Age-Related Macular Degeneration
title_full_unstemmed	Effects of Vitamin D3 and Meso-Zeaxanthin on Human Retinal Pigmented Epithelial Cells in Three Integrated in vitro Paradigms of Age-Related Macular Degeneration
title_sort	effects of vitamin d3 and meso-zeaxanthin on human retinal pigmented epithelial cells in three integrated in vitro paradigms of age-related macular degeneration
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/5993f80cbbba4e4388f14a9be33dec41
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Effects of Vitamin D3 and Meso-Zeaxanthin on Human Retinal Pigmented Epithelial Cells in Three Integrated in vitro Paradigms of Age-Related Macular Degeneration

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