NEAP/DUSP26 suppresses receptor tyrosine kinases and regulates neuronal development in zebrafish
Abstract Expression of neuroendocrine-associated phosphatase (NEAP, also named as dual specificity phosphatase 26, [DUSP26]) is restricted to neuroendocrine tissues. We found that NEAP, but not its phosphatase-defective mutant, suppressed nerve growth factor (NGF) receptor TrkA and fibroblast growth...
Guardado en:
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2017
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/599719b39d684782913c9df55d9b0cf0 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:599719b39d684782913c9df55d9b0cf0 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:599719b39d684782913c9df55d9b0cf02021-12-02T16:06:32ZNEAP/DUSP26 suppresses receptor tyrosine kinases and regulates neuronal development in zebrafish10.1038/s41598-017-05584-72045-2322https://doaj.org/article/599719b39d684782913c9df55d9b0cf02017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05584-7https://doaj.org/toc/2045-2322Abstract Expression of neuroendocrine-associated phosphatase (NEAP, also named as dual specificity phosphatase 26, [DUSP26]) is restricted to neuroendocrine tissues. We found that NEAP, but not its phosphatase-defective mutant, suppressed nerve growth factor (NGF) receptor TrkA and fibroblast growth factor receptor 1 (FGFR1) activation in PC12 cells upon NGF stimulation. Conversely, suppressing NEAP expression by RNA interference enhanced TrkA and FGFR1 phosphorylation. NEAP was capable of de-phosphorylating TrkA and FGFR1 directly in vitro. NEAP-orthologous gene existed in zebrafish. Morpholino (MO) suppression of NEAP in zebrafish resulted in hyper-phosphorylation of TrkA and FGFR1 as well as abnormal body postures and small eyes. Differentiation of retina in zebrafishes with NEAP MO treatment was severely defective, so were cranial motor neurons. Taken together, our data indicated that NEAP/DUSP26 have a critical role in regulating TrkA and FGFR1 signaling as well as proper development of retina and neuronal system in zebrafish.Chi-Hwa YangYu-Jung YehJiz-Yuh WangYa-Wen LiuYen-Lin ChenHui-Wen ChengChun-Mei ChengYung-Jen ChuangChiou-Hwa YuhYi-Rong ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Chi-Hwa Yang Yu-Jung Yeh Jiz-Yuh Wang Ya-Wen Liu Yen-Lin Chen Hui-Wen Cheng Chun-Mei Cheng Yung-Jen Chuang Chiou-Hwa Yuh Yi-Rong Chen NEAP/DUSP26 suppresses receptor tyrosine kinases and regulates neuronal development in zebrafish |
| description |
Abstract Expression of neuroendocrine-associated phosphatase (NEAP, also named as dual specificity phosphatase 26, [DUSP26]) is restricted to neuroendocrine tissues. We found that NEAP, but not its phosphatase-defective mutant, suppressed nerve growth factor (NGF) receptor TrkA and fibroblast growth factor receptor 1 (FGFR1) activation in PC12 cells upon NGF stimulation. Conversely, suppressing NEAP expression by RNA interference enhanced TrkA and FGFR1 phosphorylation. NEAP was capable of de-phosphorylating TrkA and FGFR1 directly in vitro. NEAP-orthologous gene existed in zebrafish. Morpholino (MO) suppression of NEAP in zebrafish resulted in hyper-phosphorylation of TrkA and FGFR1 as well as abnormal body postures and small eyes. Differentiation of retina in zebrafishes with NEAP MO treatment was severely defective, so were cranial motor neurons. Taken together, our data indicated that NEAP/DUSP26 have a critical role in regulating TrkA and FGFR1 signaling as well as proper development of retina and neuronal system in zebrafish. |
| format |
article |
| author |
Chi-Hwa Yang Yu-Jung Yeh Jiz-Yuh Wang Ya-Wen Liu Yen-Lin Chen Hui-Wen Cheng Chun-Mei Cheng Yung-Jen Chuang Chiou-Hwa Yuh Yi-Rong Chen |
| author_facet |
Chi-Hwa Yang Yu-Jung Yeh Jiz-Yuh Wang Ya-Wen Liu Yen-Lin Chen Hui-Wen Cheng Chun-Mei Cheng Yung-Jen Chuang Chiou-Hwa Yuh Yi-Rong Chen |
| author_sort |
Chi-Hwa Yang |
| title |
NEAP/DUSP26 suppresses receptor tyrosine kinases and regulates neuronal development in zebrafish |
| title_short |
NEAP/DUSP26 suppresses receptor tyrosine kinases and regulates neuronal development in zebrafish |
| title_full |
NEAP/DUSP26 suppresses receptor tyrosine kinases and regulates neuronal development in zebrafish |
| title_fullStr |
NEAP/DUSP26 suppresses receptor tyrosine kinases and regulates neuronal development in zebrafish |
| title_full_unstemmed |
NEAP/DUSP26 suppresses receptor tyrosine kinases and regulates neuronal development in zebrafish |
| title_sort |
neap/dusp26 suppresses receptor tyrosine kinases and regulates neuronal development in zebrafish |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/599719b39d684782913c9df55d9b0cf0 |
| work_keys_str_mv |
AT chihwayang neapdusp26suppressesreceptortyrosinekinasesandregulatesneuronaldevelopmentinzebrafish AT yujungyeh neapdusp26suppressesreceptortyrosinekinasesandregulatesneuronaldevelopmentinzebrafish AT jizyuhwang neapdusp26suppressesreceptortyrosinekinasesandregulatesneuronaldevelopmentinzebrafish AT yawenliu neapdusp26suppressesreceptortyrosinekinasesandregulatesneuronaldevelopmentinzebrafish AT yenlinchen neapdusp26suppressesreceptortyrosinekinasesandregulatesneuronaldevelopmentinzebrafish AT huiwencheng neapdusp26suppressesreceptortyrosinekinasesandregulatesneuronaldevelopmentinzebrafish AT chunmeicheng neapdusp26suppressesreceptortyrosinekinasesandregulatesneuronaldevelopmentinzebrafish AT yungjenchuang neapdusp26suppressesreceptortyrosinekinasesandregulatesneuronaldevelopmentinzebrafish AT chiouhwayuh neapdusp26suppressesreceptortyrosinekinasesandregulatesneuronaldevelopmentinzebrafish AT yirongchen neapdusp26suppressesreceptortyrosinekinasesandregulatesneuronaldevelopmentinzebrafish |
| _version_ |
1718384961770225664 |