Lineage tracing using a Cas9-deaminase barcoding system targeting endogenous L1 elements

Lineage tracing has provided new insights into cell fate but defining cellular diversity remains a challenge. Here the authors target endogenous repeat regions in mammalian cells with cytidine deaminase fused to nCas9 to create genetic barcodes for fine-resolution mapping.

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Autores principales:	Byungjin Hwang, Wookjae Lee, Soo-Young Yum, Yujin Jeon, Namjin Cho, Goo Jang, Duhee Bang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/59973f013a274b7f970aafea760c6a65
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spelling	oai:doaj.org-article:59973f013a274b7f970aafea760c6a652021-12-02T17:01:45ZLineage tracing using a Cas9-deaminase barcoding system targeting endogenous L1 elements10.1038/s41467-019-09203-z2041-1723https://doaj.org/article/59973f013a274b7f970aafea760c6a652019-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09203-zhttps://doaj.org/toc/2041-1723Lineage tracing has provided new insights into cell fate but defining cellular diversity remains a challenge. Here the authors target endogenous repeat regions in mammalian cells with cytidine deaminase fused to nCas9 to create genetic barcodes for fine-resolution mapping.Byungjin HwangWookjae LeeSoo-Young YumYujin JeonNamjin ChoGoo JangDuhee BangNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Byungjin Hwang Wookjae Lee Soo-Young Yum Yujin Jeon Namjin Cho Goo Jang Duhee Bang Lineage tracing using a Cas9-deaminase barcoding system targeting endogenous L1 elements
description	Lineage tracing has provided new insights into cell fate but defining cellular diversity remains a challenge. Here the authors target endogenous repeat regions in mammalian cells with cytidine deaminase fused to nCas9 to create genetic barcodes for fine-resolution mapping.
format	article
author	Byungjin Hwang Wookjae Lee Soo-Young Yum Yujin Jeon Namjin Cho Goo Jang Duhee Bang
author_facet	Byungjin Hwang Wookjae Lee Soo-Young Yum Yujin Jeon Namjin Cho Goo Jang Duhee Bang
author_sort	Byungjin Hwang
title	Lineage tracing using a Cas9-deaminase barcoding system targeting endogenous L1 elements
title_short	Lineage tracing using a Cas9-deaminase barcoding system targeting endogenous L1 elements
title_full	Lineage tracing using a Cas9-deaminase barcoding system targeting endogenous L1 elements
title_fullStr	Lineage tracing using a Cas9-deaminase barcoding system targeting endogenous L1 elements
title_full_unstemmed	Lineage tracing using a Cas9-deaminase barcoding system targeting endogenous L1 elements
title_sort	lineage tracing using a cas9-deaminase barcoding system targeting endogenous l1 elements
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/59973f013a274b7f970aafea760c6a65
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Lineage tracing using a Cas9-deaminase barcoding system targeting endogenous L1 elements

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