Differential effects of activated human renal epithelial cells on T-cell migration.

<h4>Background</h4>Renal tubular epithelial cells (TECs) are one of the main targets of inflammatory insults during interstitial nephritis and kidney transplant rejection. While Th1 cells are know to be essential in the pathogenesis of rejection, the role of Th17 is still under debate. W...

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Autores principales:	Martijn W H J Demmers, Carla C Baan, Els van Beelen, Jan N M Ijzermans, Willem Weimar, Ajda T Rowshani
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/59a040caa4cf46b9b92fe54600e3d07c
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spelling	oai:doaj.org-article:59a040caa4cf46b9b92fe54600e3d07c2021-11-18T07:44:29ZDifferential effects of activated human renal epithelial cells on T-cell migration.1932-620310.1371/journal.pone.0064916https://doaj.org/article/59a040caa4cf46b9b92fe54600e3d07c2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23717673/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Renal tubular epithelial cells (TECs) are one of the main targets of inflammatory insults during interstitial nephritis and kidney transplant rejection. While Th1 cells are know to be essential in the pathogenesis of rejection, the role of Th17 is still under debate. We hypothesize that TECs modulate the outcome of rejection process by production of distinct chemokines and cytokines that determine the attraction of different T-cell subsets. Therefore, we studied differential effects of activated human renal epithelial cells on T-cell migration.<h4>Methods</h4>Human primary TECs were stimulated by IFN-γ and TNF-α in vitro. Chemokines and cytokines produced by activated TECs were measured using Luminex or ELISA. Chemotaxis assay was performed using activated peripheral blood mononuclear cells composed of CD4+CXCR3+ and CD4+CCR6+ T cells migrating towards stimulated and unstimulated TECs.<h4>Results</h4>While activated TECs secreted abundant amounts of the pro-inflammatory cytokines IL-6 and IL-8, the T helper cell differentiation cytokines IL-1β, IL-12p70, IL-23 or TGF-β1 were not produced. The production of Th1 chemokines CXCL9, CXCL10 and CCL5 were significantly upregulated after TEC stimulation. In contrast, Th17 chemokine CCL20 could not be detected. Finally, activated TECs attracted significantly higher numbers of CD4+CXCR3+ T cells as compared to unstimulated TECs. No migration of CD4+CCR6+ T cells could be observed.<h4>Conclusion</h4>Activated primary renal tubular epithelial cells do not attract Th17 cells nor produce cytokines promoting Th17 cell differentiation in our experimental system mimicking the proinflammatory microenvironment of rejection.Martijn W H J DemmersCarla C BaanEls van BeelenJan N M IjzermansWillem WeimarAjda T RowshaniPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e64916 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Martijn W H J Demmers Carla C Baan Els van Beelen Jan N M Ijzermans Willem Weimar Ajda T Rowshani Differential effects of activated human renal epithelial cells on T-cell migration.
description	<h4>Background</h4>Renal tubular epithelial cells (TECs) are one of the main targets of inflammatory insults during interstitial nephritis and kidney transplant rejection. While Th1 cells are know to be essential in the pathogenesis of rejection, the role of Th17 is still under debate. We hypothesize that TECs modulate the outcome of rejection process by production of distinct chemokines and cytokines that determine the attraction of different T-cell subsets. Therefore, we studied differential effects of activated human renal epithelial cells on T-cell migration.<h4>Methods</h4>Human primary TECs were stimulated by IFN-γ and TNF-α in vitro. Chemokines and cytokines produced by activated TECs were measured using Luminex or ELISA. Chemotaxis assay was performed using activated peripheral blood mononuclear cells composed of CD4+CXCR3+ and CD4+CCR6+ T cells migrating towards stimulated and unstimulated TECs.<h4>Results</h4>While activated TECs secreted abundant amounts of the pro-inflammatory cytokines IL-6 and IL-8, the T helper cell differentiation cytokines IL-1β, IL-12p70, IL-23 or TGF-β1 were not produced. The production of Th1 chemokines CXCL9, CXCL10 and CCL5 were significantly upregulated after TEC stimulation. In contrast, Th17 chemokine CCL20 could not be detected. Finally, activated TECs attracted significantly higher numbers of CD4+CXCR3+ T cells as compared to unstimulated TECs. No migration of CD4+CCR6+ T cells could be observed.<h4>Conclusion</h4>Activated primary renal tubular epithelial cells do not attract Th17 cells nor produce cytokines promoting Th17 cell differentiation in our experimental system mimicking the proinflammatory microenvironment of rejection.
format	article
author	Martijn W H J Demmers Carla C Baan Els van Beelen Jan N M Ijzermans Willem Weimar Ajda T Rowshani
author_facet	Martijn W H J Demmers Carla C Baan Els van Beelen Jan N M Ijzermans Willem Weimar Ajda T Rowshani
author_sort	Martijn W H J Demmers
title	Differential effects of activated human renal epithelial cells on T-cell migration.
title_short	Differential effects of activated human renal epithelial cells on T-cell migration.
title_full	Differential effects of activated human renal epithelial cells on T-cell migration.
title_fullStr	Differential effects of activated human renal epithelial cells on T-cell migration.
title_full_unstemmed	Differential effects of activated human renal epithelial cells on T-cell migration.
title_sort	differential effects of activated human renal epithelial cells on t-cell migration.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/59a040caa4cf46b9b92fe54600e3d07c
work_keys_str_mv	AT martijnwhjdemmers differentialeffectsofactivatedhumanrenalepithelialcellsontcellmigration AT carlacbaan differentialeffectsofactivatedhumanrenalepithelialcellsontcellmigration AT elsvanbeelen differentialeffectsofactivatedhumanrenalepithelialcellsontcellmigration AT jannmijzermans differentialeffectsofactivatedhumanrenalepithelialcellsontcellmigration AT willemweimar differentialeffectsofactivatedhumanrenalepithelialcellsontcellmigration AT ajdatrowshani differentialeffectsofactivatedhumanrenalepithelialcellsontcellmigration
_version_	1718423037731143680

Differential effects of activated human renal epithelial cells on T-cell migration.

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