TGFβ-induced osteogenic potential of human amniotic fluid stem cells via CD73-generated adenosine production

Abstract The human amniotic fluid stem cell (hAFSC) population consists of two morphologically distinct subtypes, spindle-shaped and round-shaped cells (SS-hAFSCs and RS-hAFSCs). Whilst SS-hAFSCs are routinely expanded in mesenchymal-type (MT) conditions, we previously showed that they acquire broad...

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Autores principales:	Kwan-Leong Hau, Anna Maria Ranzoni, Filipa Vlahova, Kate Hawkins, Paolo De Coppi, Anna L. David, Pascale V. Guillot
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/59a13a03c1544427a4059428931264d4
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spelling	oai:doaj.org-article:59a13a03c1544427a4059428931264d42021-12-02T15:06:16ZTGFβ-induced osteogenic potential of human amniotic fluid stem cells via CD73-generated adenosine production10.1038/s41598-017-06780-12045-2322https://doaj.org/article/59a13a03c1544427a4059428931264d42017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06780-1https://doaj.org/toc/2045-2322Abstract The human amniotic fluid stem cell (hAFSC) population consists of two morphologically distinct subtypes, spindle-shaped and round-shaped cells (SS-hAFSCs and RS-hAFSCs). Whilst SS-hAFSCs are routinely expanded in mesenchymal-type (MT) conditions, we previously showed that they acquire broader differentiation potential when cultured under embryonic-type (ET) conditions. However, the effects of culture conditions on RS-hAFSCs have not been determined. Here, we show that culturing RS-hAFSCs under ET conditions confers faster proliferation and enhances the efficiency of osteogenic differentiation of the cells. We show that this occurs via TGFβ-induced activation of CD73 and the associated increase in the generation of extracellular adenosine. Our data demonstrate that culture conditions are decisive for the expansion of hAFSCs and that TGFβ present in ET conditions causes the phenotype of RS-hAFSCs to revert to an earlier state of stemness. Cultivating RS-hAFSCs in ET conditions with TGFβ may therefore increase their therapeutic potential for clinical applications.Kwan-Leong HauAnna Maria RanzoniFilipa VlahovaKate HawkinsPaolo De CoppiAnna L. DavidPascale V. GuillotNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kwan-Leong Hau Anna Maria Ranzoni Filipa Vlahova Kate Hawkins Paolo De Coppi Anna L. David Pascale V. Guillot TGFβ-induced osteogenic potential of human amniotic fluid stem cells via CD73-generated adenosine production
description	Abstract The human amniotic fluid stem cell (hAFSC) population consists of two morphologically distinct subtypes, spindle-shaped and round-shaped cells (SS-hAFSCs and RS-hAFSCs). Whilst SS-hAFSCs are routinely expanded in mesenchymal-type (MT) conditions, we previously showed that they acquire broader differentiation potential when cultured under embryonic-type (ET) conditions. However, the effects of culture conditions on RS-hAFSCs have not been determined. Here, we show that culturing RS-hAFSCs under ET conditions confers faster proliferation and enhances the efficiency of osteogenic differentiation of the cells. We show that this occurs via TGFβ-induced activation of CD73 and the associated increase in the generation of extracellular adenosine. Our data demonstrate that culture conditions are decisive for the expansion of hAFSCs and that TGFβ present in ET conditions causes the phenotype of RS-hAFSCs to revert to an earlier state of stemness. Cultivating RS-hAFSCs in ET conditions with TGFβ may therefore increase their therapeutic potential for clinical applications.
format	article
author	Kwan-Leong Hau Anna Maria Ranzoni Filipa Vlahova Kate Hawkins Paolo De Coppi Anna L. David Pascale V. Guillot
author_facet	Kwan-Leong Hau Anna Maria Ranzoni Filipa Vlahova Kate Hawkins Paolo De Coppi Anna L. David Pascale V. Guillot
author_sort	Kwan-Leong Hau
title	TGFβ-induced osteogenic potential of human amniotic fluid stem cells via CD73-generated adenosine production
title_short	TGFβ-induced osteogenic potential of human amniotic fluid stem cells via CD73-generated adenosine production
title_full	TGFβ-induced osteogenic potential of human amniotic fluid stem cells via CD73-generated adenosine production
title_fullStr	TGFβ-induced osteogenic potential of human amniotic fluid stem cells via CD73-generated adenosine production
title_full_unstemmed	TGFβ-induced osteogenic potential of human amniotic fluid stem cells via CD73-generated adenosine production
title_sort	tgfβ-induced osteogenic potential of human amniotic fluid stem cells via cd73-generated adenosine production
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/59a13a03c1544427a4059428931264d4
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TGFβ-induced osteogenic potential of human amniotic fluid stem cells via CD73-generated adenosine production

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