Molecular surveillance of shiga toxigenic Escherichia coli in selected beef abattoirs in Osun State Nigeria

Abstract Shiga toxigenic strains of E. coli (STEC) known to be etiological agents for diarrhea were screened for their incidence/occurrence in selected abattoirs sources in Osogbo metropolis of Osun State, Nigeria using a randomized block design. Samples were plated directly on selective and differe...

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Autores principales:	Femi Ayoade, Judith Oguzie, Philomena Eromon, Omolola E. Omotosho, Tosin Ogunbiyi, Testimony Olumade, Kazeem Akano, Onikepe Folarin, Christian Happi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/59a51e4d78634b7598752ee487b33739
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spelling	oai:doaj.org-article:59a51e4d78634b7598752ee487b337392021-12-02T16:24:49ZMolecular surveillance of shiga toxigenic Escherichia coli in selected beef abattoirs in Osun State Nigeria10.1038/s41598-021-93347-w2045-2322https://doaj.org/article/59a51e4d78634b7598752ee487b337392021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93347-whttps://doaj.org/toc/2045-2322Abstract Shiga toxigenic strains of E. coli (STEC) known to be etiological agents for diarrhea were screened for their incidence/occurrence in selected abattoirs sources in Osogbo metropolis of Osun State, Nigeria using a randomized block design. Samples were plated directly on selective and differential media and E. coli isolates. Multiplex PCR analysis was used to screen for the presence of specific virulence factors. These were confirmed serologically as non-O157 STEC using latex agglutination serotyping kit. Sequence analysis of PCR products was performed on a representative isolate showing the highest combination of virulence genes using the 16S gene for identification purposes only. Results showed that the average cfu/cm2 was significantly lower in the samples collected at Sekona-2 slaughter slab compared with those collected at Al-maleek batch abattoir and Sekona-1 slaughter slab in ascending order at P = 0.03. Moreover, the average cfu/cm2 E. coli in samples collected from butchering knife was significantly lower when compared with that of the workers’ hand (P = 0.047) and slaughtering floor (P = 0.047) but not with the slaughter table (P = 0.98) and effluent water from the abattoir house (P = 0.39). These data suggest that the abattoir type may not be as important in the prevalence and spread of STEC as the hygiene practices of the workers. Sequence analysis of a representative isolate showed 100% coverage and 96.46% percentage identity with Escherichia coli O113:H21 (GenBank Accession number: CP031892.1) strain from Canada. This sequence was subsequently submitted to GenBank with accession number MW463885. From evolutionary analyses, the strain from Nigeria, sequenced in this study, is evolutionarily distant when compared with the publicly available sequences from Nigeria. Although no case of E. coli O157 was found within the study area, percent occurrence of non-O157 STEC as high as 46.3% at some of the sampled sites is worrisome and requires regulatory interventions in ensuring hygienic practices at the abattoirs within the study area.Femi AyoadeJudith OguziePhilomena EromonOmolola E. OmotoshoTosin OgunbiyiTestimony OlumadeKazeem AkanoOnikepe FolarinChristian HappiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Femi Ayoade Judith Oguzie Philomena Eromon Omolola E. Omotosho Tosin Ogunbiyi Testimony Olumade Kazeem Akano Onikepe Folarin Christian Happi Molecular surveillance of shiga toxigenic Escherichia coli in selected beef abattoirs in Osun State Nigeria
description	Abstract Shiga toxigenic strains of E. coli (STEC) known to be etiological agents for diarrhea were screened for their incidence/occurrence in selected abattoirs sources in Osogbo metropolis of Osun State, Nigeria using a randomized block design. Samples were plated directly on selective and differential media and E. coli isolates. Multiplex PCR analysis was used to screen for the presence of specific virulence factors. These were confirmed serologically as non-O157 STEC using latex agglutination serotyping kit. Sequence analysis of PCR products was performed on a representative isolate showing the highest combination of virulence genes using the 16S gene for identification purposes only. Results showed that the average cfu/cm2 was significantly lower in the samples collected at Sekona-2 slaughter slab compared with those collected at Al-maleek batch abattoir and Sekona-1 slaughter slab in ascending order at P = 0.03. Moreover, the average cfu/cm2 E. coli in samples collected from butchering knife was significantly lower when compared with that of the workers’ hand (P = 0.047) and slaughtering floor (P = 0.047) but not with the slaughter table (P = 0.98) and effluent water from the abattoir house (P = 0.39). These data suggest that the abattoir type may not be as important in the prevalence and spread of STEC as the hygiene practices of the workers. Sequence analysis of a representative isolate showed 100% coverage and 96.46% percentage identity with Escherichia coli O113:H21 (GenBank Accession number: CP031892.1) strain from Canada. This sequence was subsequently submitted to GenBank with accession number MW463885. From evolutionary analyses, the strain from Nigeria, sequenced in this study, is evolutionarily distant when compared with the publicly available sequences from Nigeria. Although no case of E. coli O157 was found within the study area, percent occurrence of non-O157 STEC as high as 46.3% at some of the sampled sites is worrisome and requires regulatory interventions in ensuring hygienic practices at the abattoirs within the study area.
format	article
author	Femi Ayoade Judith Oguzie Philomena Eromon Omolola E. Omotosho Tosin Ogunbiyi Testimony Olumade Kazeem Akano Onikepe Folarin Christian Happi
author_facet	Femi Ayoade Judith Oguzie Philomena Eromon Omolola E. Omotosho Tosin Ogunbiyi Testimony Olumade Kazeem Akano Onikepe Folarin Christian Happi
author_sort	Femi Ayoade
title	Molecular surveillance of shiga toxigenic Escherichia coli in selected beef abattoirs in Osun State Nigeria
title_short	Molecular surveillance of shiga toxigenic Escherichia coli in selected beef abattoirs in Osun State Nigeria
title_full	Molecular surveillance of shiga toxigenic Escherichia coli in selected beef abattoirs in Osun State Nigeria
title_fullStr	Molecular surveillance of shiga toxigenic Escherichia coli in selected beef abattoirs in Osun State Nigeria
title_full_unstemmed	Molecular surveillance of shiga toxigenic Escherichia coli in selected beef abattoirs in Osun State Nigeria
title_sort	molecular surveillance of shiga toxigenic escherichia coli in selected beef abattoirs in osun state nigeria
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/59a51e4d78634b7598752ee487b33739
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Molecular surveillance of shiga toxigenic Escherichia coli in selected beef abattoirs in Osun State Nigeria

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