miR-92a-3p Promoted EMT via Targeting LATS1 in Cervical Cancer Stem Cells

miR-92a-3p (microRNA-92a-3p) has been reported to be dysregulated in several cancers, and as such, it is considered to be a cancer-related microRNA. However, the influence of miR-92a-3p on biological behaviors in cervical cancer (CC) still remains unclear. Quantitative real-time PCR was used to dete...

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Autores principales:	Shuangyue Liu, Liping Chu, Mingzhu Xie, Lisha Ma, Hongmei An, Wen Zhang, Jihong Deng
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	miR-92a-3p larger tumor suppressor cervical cancer E-cadherin EMT Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/59a576644c93473e909a60636f9b03b6
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spelling	oai:doaj.org-article:59a576644c93473e909a60636f9b03b62021-11-18T09:44:51ZmiR-92a-3p Promoted EMT via Targeting LATS1 in Cervical Cancer Stem Cells2296-634X10.3389/fcell.2021.757747https://doaj.org/article/59a576644c93473e909a60636f9b03b62021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.757747/fullhttps://doaj.org/toc/2296-634XmiR-92a-3p (microRNA-92a-3p) has been reported to be dysregulated in several cancers, and as such, it is considered to be a cancer-related microRNA. However, the influence of miR-92a-3p on biological behaviors in cervical cancer (CC) still remains unclear. Quantitative real-time PCR was used to detect miR-92a-3p levels in CC stem cells. Here, Cell Counting Kit-8 (CCK8) assay, Transwell cell invasion assay and flow cytometry assay were used to characterize the effects that miR-92a-3p and large tumor suppressor l (LATS1) had on proliferation, invasion and cell cycle transition. The luciferase reporter gene assay was used to verify the targeting relationship between miR-92a-3p and LATS1. Western Blotting was used to investigate the related signaling pathways and proteins. Data from The Cancer Genome Atlas (TCGA) showed that miR-92a-3p was upregulated in CC tissues and closely associated with overall survival. miR-92a-3p promoted proliferation, invasion and cell cycle transition in CC stem cells. The luciferase reporter assay showed that miR-92a-3p bound to the 3′-untranslated region (3′-UTR) of the LATS1 promoter. LATS1 inhibited proliferation, invasion and cell cycle transition. Results measured by Western Blotting showed that LATS1 downregulated expressions of transcriptional co-activator with PDZ-binding motif (TAZ), vimentin and cyclin E, but upregulated the expression of E-cadherin. Re-expression of LATS1 partly reversed the effects of miR-92a-3p on proliferation, invasion and cell cycle transition, as well as on TAZ, E-cadherin, vimentin, and cyclin E. miR-92a-3p promoted the malignant behavior of CC stem cells by targeting LATS1, which regulated TAZ and E-cadherin.Shuangyue LiuLiping ChuMingzhu XieLisha MaHongmei AnWen ZhangJihong DengFrontiers Media S.A.articlemiR-92a-3plarger tumor suppressorcervical cancerE-cadherinEMTBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	miR-92a-3p larger tumor suppressor cervical cancer E-cadherin EMT Biology (General) QH301-705.5
spellingShingle	miR-92a-3p larger tumor suppressor cervical cancer E-cadherin EMT Biology (General) QH301-705.5 Shuangyue Liu Liping Chu Mingzhu Xie Lisha Ma Hongmei An Wen Zhang Jihong Deng miR-92a-3p Promoted EMT via Targeting LATS1 in Cervical Cancer Stem Cells
description	miR-92a-3p (microRNA-92a-3p) has been reported to be dysregulated in several cancers, and as such, it is considered to be a cancer-related microRNA. However, the influence of miR-92a-3p on biological behaviors in cervical cancer (CC) still remains unclear. Quantitative real-time PCR was used to detect miR-92a-3p levels in CC stem cells. Here, Cell Counting Kit-8 (CCK8) assay, Transwell cell invasion assay and flow cytometry assay were used to characterize the effects that miR-92a-3p and large tumor suppressor l (LATS1) had on proliferation, invasion and cell cycle transition. The luciferase reporter gene assay was used to verify the targeting relationship between miR-92a-3p and LATS1. Western Blotting was used to investigate the related signaling pathways and proteins. Data from The Cancer Genome Atlas (TCGA) showed that miR-92a-3p was upregulated in CC tissues and closely associated with overall survival. miR-92a-3p promoted proliferation, invasion and cell cycle transition in CC stem cells. The luciferase reporter assay showed that miR-92a-3p bound to the 3′-untranslated region (3′-UTR) of the LATS1 promoter. LATS1 inhibited proliferation, invasion and cell cycle transition. Results measured by Western Blotting showed that LATS1 downregulated expressions of transcriptional co-activator with PDZ-binding motif (TAZ), vimentin and cyclin E, but upregulated the expression of E-cadherin. Re-expression of LATS1 partly reversed the effects of miR-92a-3p on proliferation, invasion and cell cycle transition, as well as on TAZ, E-cadherin, vimentin, and cyclin E. miR-92a-3p promoted the malignant behavior of CC stem cells by targeting LATS1, which regulated TAZ and E-cadherin.
format	article
author	Shuangyue Liu Liping Chu Mingzhu Xie Lisha Ma Hongmei An Wen Zhang Jihong Deng
author_facet	Shuangyue Liu Liping Chu Mingzhu Xie Lisha Ma Hongmei An Wen Zhang Jihong Deng
author_sort	Shuangyue Liu
title	miR-92a-3p Promoted EMT via Targeting LATS1 in Cervical Cancer Stem Cells
title_short	miR-92a-3p Promoted EMT via Targeting LATS1 in Cervical Cancer Stem Cells
title_full	miR-92a-3p Promoted EMT via Targeting LATS1 in Cervical Cancer Stem Cells
title_fullStr	miR-92a-3p Promoted EMT via Targeting LATS1 in Cervical Cancer Stem Cells
title_full_unstemmed	miR-92a-3p Promoted EMT via Targeting LATS1 in Cervical Cancer Stem Cells
title_sort	mir-92a-3p promoted emt via targeting lats1 in cervical cancer stem cells
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/59a576644c93473e909a60636f9b03b6
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miR-92a-3p Promoted EMT via Targeting LATS1 in Cervical Cancer Stem Cells

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