Visible Light-Activated Carbon Dots for Inhibiting Biofilm Formation and Inactivating Biofilm-Associated Bacterial Cells

This study aimed to address the significant problems of bacterial biofilms found in medical fields and many industries. It explores the potential of classic photoactive carbon dots (CDots), with 2,2′-(ethylenedioxy)bis (ethylamine) (EDA) for dot surface functionalization (thus, EDA-CDots) for their...

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Autores principales:	Xiuli Dong, Christopher M. Overton, Yongan Tang, Jasmine P. Darby, Ya-Ping Sun, Liju Yang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	biofilm carbon dots inactivation inhibition photoactive Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/59a68286620643329094da7002ef3d9c
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spelling	oai:doaj.org-article:59a68286620643329094da7002ef3d9c2021-11-18T07:26:36ZVisible Light-Activated Carbon Dots for Inhibiting Biofilm Formation and Inactivating Biofilm-Associated Bacterial Cells2296-418510.3389/fbioe.2021.786077https://doaj.org/article/59a68286620643329094da7002ef3d9c2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.786077/fullhttps://doaj.org/toc/2296-4185This study aimed to address the significant problems of bacterial biofilms found in medical fields and many industries. It explores the potential of classic photoactive carbon dots (CDots), with 2,2′-(ethylenedioxy)bis (ethylamine) (EDA) for dot surface functionalization (thus, EDA-CDots) for their inhibitory effect on B. subtilis biofilm formation and the inactivation of B. subtilis cells within established biofilm. The EDA-CDots were synthesized by chemical functionalization of selected small carbon nanoparticles with EDA molecules in amidation reactions. The inhibitory efficacy of CDots with visible light against biofilm formation was dependent significantly on the time point when CDots were added; the earlier the CDots were added, the better the inhibitory effect on the biofilm formation. The evaluation of antibacterial action of light-activated EDA-CDots against planktonic B. subtilis cells versus the cells in biofilm indicate that CDots are highly effective for inactivating planktonic cells but barely inactivate cells in established biofilms. However, when coupling with chelating agents (e.g., EDTA) to target the biofilm architecture by breaking or weakening the EPS protection, much enhanced photoinactivation of biofilm-associated cells by CDots was achieved. The study demonstrates the potential of CDots to prevent the initiation of biofilm formation and to inhibit biofilm growth at an early stage. Strategic combination treatment could enhance the effectiveness of photoinactivation by CDots to biofilm-associated cells.Xiuli DongChristopher M. OvertonYongan TangJasmine P. DarbyYa-Ping SunLiju YangFrontiers Media S.A.articlebiofilmcarbon dotsinactivationinhibitionphotoactiveBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biofilm carbon dots inactivation inhibition photoactive Biotechnology TP248.13-248.65
spellingShingle	biofilm carbon dots inactivation inhibition photoactive Biotechnology TP248.13-248.65 Xiuli Dong Christopher M. Overton Yongan Tang Jasmine P. Darby Ya-Ping Sun Liju Yang Visible Light-Activated Carbon Dots for Inhibiting Biofilm Formation and Inactivating Biofilm-Associated Bacterial Cells
description	This study aimed to address the significant problems of bacterial biofilms found in medical fields and many industries. It explores the potential of classic photoactive carbon dots (CDots), with 2,2′-(ethylenedioxy)bis (ethylamine) (EDA) for dot surface functionalization (thus, EDA-CDots) for their inhibitory effect on B. subtilis biofilm formation and the inactivation of B. subtilis cells within established biofilm. The EDA-CDots were synthesized by chemical functionalization of selected small carbon nanoparticles with EDA molecules in amidation reactions. The inhibitory efficacy of CDots with visible light against biofilm formation was dependent significantly on the time point when CDots were added; the earlier the CDots were added, the better the inhibitory effect on the biofilm formation. The evaluation of antibacterial action of light-activated EDA-CDots against planktonic B. subtilis cells versus the cells in biofilm indicate that CDots are highly effective for inactivating planktonic cells but barely inactivate cells in established biofilms. However, when coupling with chelating agents (e.g., EDTA) to target the biofilm architecture by breaking or weakening the EPS protection, much enhanced photoinactivation of biofilm-associated cells by CDots was achieved. The study demonstrates the potential of CDots to prevent the initiation of biofilm formation and to inhibit biofilm growth at an early stage. Strategic combination treatment could enhance the effectiveness of photoinactivation by CDots to biofilm-associated cells.
format	article
author	Xiuli Dong Christopher M. Overton Yongan Tang Jasmine P. Darby Ya-Ping Sun Liju Yang
author_facet	Xiuli Dong Christopher M. Overton Yongan Tang Jasmine P. Darby Ya-Ping Sun Liju Yang
author_sort	Xiuli Dong
title	Visible Light-Activated Carbon Dots for Inhibiting Biofilm Formation and Inactivating Biofilm-Associated Bacterial Cells
title_short	Visible Light-Activated Carbon Dots for Inhibiting Biofilm Formation and Inactivating Biofilm-Associated Bacterial Cells
title_full	Visible Light-Activated Carbon Dots for Inhibiting Biofilm Formation and Inactivating Biofilm-Associated Bacterial Cells
title_fullStr	Visible Light-Activated Carbon Dots for Inhibiting Biofilm Formation and Inactivating Biofilm-Associated Bacterial Cells
title_full_unstemmed	Visible Light-Activated Carbon Dots for Inhibiting Biofilm Formation and Inactivating Biofilm-Associated Bacterial Cells
title_sort	visible light-activated carbon dots for inhibiting biofilm formation and inactivating biofilm-associated bacterial cells
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/59a68286620643329094da7002ef3d9c
work_keys_str_mv	AT xiulidong visiblelightactivatedcarbondotsforinhibitingbiofilmformationandinactivatingbiofilmassociatedbacterialcells AT christophermoverton visiblelightactivatedcarbondotsforinhibitingbiofilmformationandinactivatingbiofilmassociatedbacterialcells AT yongantang visiblelightactivatedcarbondotsforinhibitingbiofilmformationandinactivatingbiofilmassociatedbacterialcells AT jasminepdarby visiblelightactivatedcarbondotsforinhibitingbiofilmformationandinactivatingbiofilmassociatedbacterialcells AT yapingsun visiblelightactivatedcarbondotsforinhibitingbiofilmformationandinactivatingbiofilmassociatedbacterialcells AT lijuyang visiblelightactivatedcarbondotsforinhibitingbiofilmformationandinactivatingbiofilmassociatedbacterialcells
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Visible Light-Activated Carbon Dots for Inhibiting Biofilm Formation and Inactivating Biofilm-Associated Bacterial Cells

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