Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective

Abstract Surface enhanced Raman scattering (SERS) nanoparticles are an attractive alternative to fluorescent probes for biological labeling because of their photostability and multiplexing capabilities. However, nanoparticle size, shape, and surface properties are known to affect nanoparticle-cell i...

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Autores principales:	Maria Navas-Moreno, Majid Mehrpouyan, Tatyana Chernenko, Demet Candas, Ming Fan, Jian Jian Li, Ming Yan, James W. Chan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/59ac85a7517141ebbc5b2a1aaaa1f6cc
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spelling	oai:doaj.org-article:59ac85a7517141ebbc5b2a1aaaa1f6cc2021-12-02T16:06:04ZNanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective10.1038/s41598-017-04066-02045-2322https://doaj.org/article/59ac85a7517141ebbc5b2a1aaaa1f6cc2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04066-0https://doaj.org/toc/2045-2322Abstract Surface enhanced Raman scattering (SERS) nanoparticles are an attractive alternative to fluorescent probes for biological labeling because of their photostability and multiplexing capabilities. However, nanoparticle size, shape, and surface properties are known to affect nanoparticle-cell interactions. Other issues such as the formation of a protein corona and antibody multivalency interfere with the labeling properties of nanoparticle-antibody conjugates. Hence, it is important to consider these aspects in order to validate such conjugates for live cell imaging applications. Using SERS nanoparticles that target HER2 and CD44 in breast cancer cells, we demonstrate labeling of fixed cells with high specificity that correlates well with fluorescent labels. However, when labeling live cells to monitor surface biomarker expression and dynamics, the nanoparticles are rapidly uptaken by the cells and become compartmentalized into different cellular regions. This behavior is in stark contrast to that of fluorescent antibody conjugates. This study highlights the impact of nanoparticle internalization and trafficking on the ability to use SERS nanoparticle-antibody conjugates to monitor cell dynamics.Maria Navas-MorenoMajid MehrpouyanTatyana ChernenkoDemet CandasMing FanJian Jian LiMing YanJames W. ChanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maria Navas-Moreno Majid Mehrpouyan Tatyana Chernenko Demet Candas Ming Fan Jian Jian Li Ming Yan James W. Chan Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective
description	Abstract Surface enhanced Raman scattering (SERS) nanoparticles are an attractive alternative to fluorescent probes for biological labeling because of their photostability and multiplexing capabilities. However, nanoparticle size, shape, and surface properties are known to affect nanoparticle-cell interactions. Other issues such as the formation of a protein corona and antibody multivalency interfere with the labeling properties of nanoparticle-antibody conjugates. Hence, it is important to consider these aspects in order to validate such conjugates for live cell imaging applications. Using SERS nanoparticles that target HER2 and CD44 in breast cancer cells, we demonstrate labeling of fixed cells with high specificity that correlates well with fluorescent labels. However, when labeling live cells to monitor surface biomarker expression and dynamics, the nanoparticles are rapidly uptaken by the cells and become compartmentalized into different cellular regions. This behavior is in stark contrast to that of fluorescent antibody conjugates. This study highlights the impact of nanoparticle internalization and trafficking on the ability to use SERS nanoparticle-antibody conjugates to monitor cell dynamics.
format	article
author	Maria Navas-Moreno Majid Mehrpouyan Tatyana Chernenko Demet Candas Ming Fan Jian Jian Li Ming Yan James W. Chan
author_facet	Maria Navas-Moreno Majid Mehrpouyan Tatyana Chernenko Demet Candas Ming Fan Jian Jian Li Ming Yan James W. Chan
author_sort	Maria Navas-Moreno
title	Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective
title_short	Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective
title_full	Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective
title_fullStr	Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective
title_full_unstemmed	Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective
title_sort	nanoparticles for live cell microscopy: a surface-enhanced raman scattering perspective
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/59ac85a7517141ebbc5b2a1aaaa1f6cc
work_keys_str_mv	AT marianavasmoreno nanoparticlesforlivecellmicroscopyasurfaceenhancedramanscatteringperspective AT majidmehrpouyan nanoparticlesforlivecellmicroscopyasurfaceenhancedramanscatteringperspective AT tatyanachernenko nanoparticlesforlivecellmicroscopyasurfaceenhancedramanscatteringperspective AT demetcandas nanoparticlesforlivecellmicroscopyasurfaceenhancedramanscatteringperspective AT mingfan nanoparticlesforlivecellmicroscopyasurfaceenhancedramanscatteringperspective AT jianjianli nanoparticlesforlivecellmicroscopyasurfaceenhancedramanscatteringperspective AT mingyan nanoparticlesforlivecellmicroscopyasurfaceenhancedramanscatteringperspective AT jameswchan nanoparticlesforlivecellmicroscopyasurfaceenhancedramanscatteringperspective
_version_	1718385090409529344

Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective

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