MicroRNA profiling in bovine serum according to the stage of Mycobacterium avium subsp. paratuberculosis infection

Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne’s disease (JD), and it causes diarrhea and weakness in cattle. During a long subclinical stage, infected animals without clinical signs shed pathogens through feces. For this reason, the diagnosis of JD during the subc...

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Autores principales:	Sung-Woon Choi, Suji Kim, Hong-Tae Park, Hyun-Eui Park, Jeong-Soo Choi, Han Sang Yoo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/59ae42508dbb4b92911dbb34d1010ef8
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spelling	oai:doaj.org-article:59ae42508dbb4b92911dbb34d1010ef82021-11-11T07:14:39ZMicroRNA profiling in bovine serum according to the stage of Mycobacterium avium subsp. paratuberculosis infection1932-6203https://doaj.org/article/59ae42508dbb4b92911dbb34d1010ef82021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8568169/?tool=EBIhttps://doaj.org/toc/1932-6203Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne’s disease (JD), and it causes diarrhea and weakness in cattle. During a long subclinical stage, infected animals without clinical signs shed pathogens through feces. For this reason, the diagnosis of JD during the subclinical stage is very important. Circulating miRNAs are attracting attention as useful biomarkers in various veterinary diseases because of their expression changes depending on the state of the disease. Based on current knowledge, circulating miRNAs extracted from bovine serum were used to develop a diagnostic tool for JD. In this study, the animals were divided into 4 groups according to fecal shedding, the presence of antibodies, and clinical signs. Gene expression was analyzed by performing miRNA sequencing for each group, and it was identified that the miRNA expression changed more as the MAP infection progressed. The eight miRNAs that were differentially expressed in all infected groups were selected as biomarker candidates based on their significant differences compared to the control group. These biomarker candidates were validated by qRT-PCR. Considering the sequencing data, two upregulated miRNAs and two downregulated miRNAs showed the same trend in the validation results. Network analysis was also conducted and the results showed that mRNAs (IL-10, TGF-β1) associated with regulatory T cells were predicted to be activated in the subclinical stage. Taken together, our data suggest that two miRNAs (bta-miR-374b, bta-miR-2887) may play major roles in the immune response to MAP infection during the subclinical stage.Sung-Woon ChoiSuji KimHong-Tae ParkHyun-Eui ParkJeong-Soo ChoiHan Sang YooPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sung-Woon Choi Suji Kim Hong-Tae Park Hyun-Eui Park Jeong-Soo Choi Han Sang Yoo MicroRNA profiling in bovine serum according to the stage of Mycobacterium avium subsp. paratuberculosis infection
description	Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne’s disease (JD), and it causes diarrhea and weakness in cattle. During a long subclinical stage, infected animals without clinical signs shed pathogens through feces. For this reason, the diagnosis of JD during the subclinical stage is very important. Circulating miRNAs are attracting attention as useful biomarkers in various veterinary diseases because of their expression changes depending on the state of the disease. Based on current knowledge, circulating miRNAs extracted from bovine serum were used to develop a diagnostic tool for JD. In this study, the animals were divided into 4 groups according to fecal shedding, the presence of antibodies, and clinical signs. Gene expression was analyzed by performing miRNA sequencing for each group, and it was identified that the miRNA expression changed more as the MAP infection progressed. The eight miRNAs that were differentially expressed in all infected groups were selected as biomarker candidates based on their significant differences compared to the control group. These biomarker candidates were validated by qRT-PCR. Considering the sequencing data, two upregulated miRNAs and two downregulated miRNAs showed the same trend in the validation results. Network analysis was also conducted and the results showed that mRNAs (IL-10, TGF-β1) associated with regulatory T cells were predicted to be activated in the subclinical stage. Taken together, our data suggest that two miRNAs (bta-miR-374b, bta-miR-2887) may play major roles in the immune response to MAP infection during the subclinical stage.
format	article
author	Sung-Woon Choi Suji Kim Hong-Tae Park Hyun-Eui Park Jeong-Soo Choi Han Sang Yoo
author_facet	Sung-Woon Choi Suji Kim Hong-Tae Park Hyun-Eui Park Jeong-Soo Choi Han Sang Yoo
author_sort	Sung-Woon Choi
title	MicroRNA profiling in bovine serum according to the stage of Mycobacterium avium subsp. paratuberculosis infection
title_short	MicroRNA profiling in bovine serum according to the stage of Mycobacterium avium subsp. paratuberculosis infection
title_full	MicroRNA profiling in bovine serum according to the stage of Mycobacterium avium subsp. paratuberculosis infection
title_fullStr	MicroRNA profiling in bovine serum according to the stage of Mycobacterium avium subsp. paratuberculosis infection
title_full_unstemmed	MicroRNA profiling in bovine serum according to the stage of Mycobacterium avium subsp. paratuberculosis infection
title_sort	microrna profiling in bovine serum according to the stage of mycobacterium avium subsp. paratuberculosis infection
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/59ae42508dbb4b92911dbb34d1010ef8
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MicroRNA profiling in bovine serum according to the stage of Mycobacterium avium subsp. paratuberculosis infection

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