Telomere associated gene expression as well as TERT protein level and telomerase activity are altered in the ovarian follicles of aged mice

Abstract Telomeres cap the ends of eukaryotic chromosomes to maintain genomic stability and integrity during an organism’s lifespan. The length of telomeres inevitably shortens due to DNA replication, genotoxic agents, and biological aging. A limited number of cell types, e.g., stem cells, germline...

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Autores principales:	Esra Gozde Kosebent, Saffet Ozturk
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/59af20eb59d24d4abf97d157b023fdb9
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spelling	oai:doaj.org-article:59af20eb59d24d4abf97d157b023fdb92021-12-02T16:23:42ZTelomere associated gene expression as well as TERT protein level and telomerase activity are altered in the ovarian follicles of aged mice10.1038/s41598-021-95239-52045-2322https://doaj.org/article/59af20eb59d24d4abf97d157b023fdb92021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95239-5https://doaj.org/toc/2045-2322Abstract Telomeres cap the ends of eukaryotic chromosomes to maintain genomic stability and integrity during an organism’s lifespan. The length of telomeres inevitably shortens due to DNA replication, genotoxic agents, and biological aging. A limited number of cell types, e.g., stem cells, germline cells, and early embryos can elongate shortened telomeres via the enzymatic action of telomerase, which is composed of telomerase reverse transcriptase (TERT) and telomerase RNA component (Terc). Additionally, telomere-associated proteins including telomeric repeat binding factor 1 (TRF1) and 2 (TRF2), as well as protection of telomeres 1a (POT1a), bind to telomeres to maintain their structural integrity and length. During ovarian aging in mammals, telomeres progressively shorten, accompanied by fertility loss; however, the molecular mechanism underlying this attrition during follicle development remains unclear. In this study, the primary, secondary, preantral, and antral follicles were obtained either from 6-week-old adult (n = 19) or 52-week-old aged (n = 12) mice. We revealed that the Tert, Terc, Trf1, Trf2, and Pot1a gene expression (P < 0.001) and TERT protein (P < 0.01) levels significantly decreased in certain ovarian follicles of the aged group when compared to those of the adult group. Also, telomerase activity exhibited remarkable changes in the follicles of both groups. Consequently, altered telomere-associated gene expression and reduced TERT protein levels in the follicles of aged mice may be a determinant of telomere shortening during ovarian aging, and infertility appearing in the later decades of reproductive lifespan. Further investigations are required to determine the molecular mechanisms underlying these alterations in the follicles during ovarian aging.Esra Gozde KosebentSaffet OzturkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Esra Gozde Kosebent Saffet Ozturk Telomere associated gene expression as well as TERT protein level and telomerase activity are altered in the ovarian follicles of aged mice
description	Abstract Telomeres cap the ends of eukaryotic chromosomes to maintain genomic stability and integrity during an organism’s lifespan. The length of telomeres inevitably shortens due to DNA replication, genotoxic agents, and biological aging. A limited number of cell types, e.g., stem cells, germline cells, and early embryos can elongate shortened telomeres via the enzymatic action of telomerase, which is composed of telomerase reverse transcriptase (TERT) and telomerase RNA component (Terc). Additionally, telomere-associated proteins including telomeric repeat binding factor 1 (TRF1) and 2 (TRF2), as well as protection of telomeres 1a (POT1a), bind to telomeres to maintain their structural integrity and length. During ovarian aging in mammals, telomeres progressively shorten, accompanied by fertility loss; however, the molecular mechanism underlying this attrition during follicle development remains unclear. In this study, the primary, secondary, preantral, and antral follicles were obtained either from 6-week-old adult (n = 19) or 52-week-old aged (n = 12) mice. We revealed that the Tert, Terc, Trf1, Trf2, and Pot1a gene expression (P < 0.001) and TERT protein (P < 0.01) levels significantly decreased in certain ovarian follicles of the aged group when compared to those of the adult group. Also, telomerase activity exhibited remarkable changes in the follicles of both groups. Consequently, altered telomere-associated gene expression and reduced TERT protein levels in the follicles of aged mice may be a determinant of telomere shortening during ovarian aging, and infertility appearing in the later decades of reproductive lifespan. Further investigations are required to determine the molecular mechanisms underlying these alterations in the follicles during ovarian aging.
format	article
author	Esra Gozde Kosebent Saffet Ozturk
author_facet	Esra Gozde Kosebent Saffet Ozturk
author_sort	Esra Gozde Kosebent
title	Telomere associated gene expression as well as TERT protein level and telomerase activity are altered in the ovarian follicles of aged mice
title_short	Telomere associated gene expression as well as TERT protein level and telomerase activity are altered in the ovarian follicles of aged mice
title_full	Telomere associated gene expression as well as TERT protein level and telomerase activity are altered in the ovarian follicles of aged mice
title_fullStr	Telomere associated gene expression as well as TERT protein level and telomerase activity are altered in the ovarian follicles of aged mice
title_full_unstemmed	Telomere associated gene expression as well as TERT protein level and telomerase activity are altered in the ovarian follicles of aged mice
title_sort	telomere associated gene expression as well as tert protein level and telomerase activity are altered in the ovarian follicles of aged mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/59af20eb59d24d4abf97d157b023fdb9
work_keys_str_mv	AT esragozdekosebent telomereassociatedgeneexpressionaswellastertproteinlevelandtelomeraseactivityarealteredintheovarianfolliclesofagedmice AT saffetozturk telomereassociatedgeneexpressionaswellastertproteinlevelandtelomeraseactivityarealteredintheovarianfolliclesofagedmice
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Telomere associated gene expression as well as TERT protein level and telomerase activity are altered in the ovarian follicles of aged mice

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