A novel fluorescent imaging technique for assessment of cerebral vasospasm after experimental subarachnoid hemorrhage

Abstract Various techniques have been developed to study changes in the cerebral vasculature in numerous neuropathological processes including subarachnoid hemorrhage (SAH). One of the most widely employed techniques uses India ink-gelatin casting, which presents numerous challenges due to its high...

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Autores principales:	Diane J. Aum, Ananth K. Vellimana, Itender Singh, Eric Milner, James W. Nelson, Byung Hee Han, Gregory J. Zipfel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/59b09322c90344f5b94a3ad3adf684d8
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spelling	oai:doaj.org-article:59b09322c90344f5b94a3ad3adf684d82021-12-02T12:32:50ZA novel fluorescent imaging technique for assessment of cerebral vasospasm after experimental subarachnoid hemorrhage10.1038/s41598-017-09070-y2045-2322https://doaj.org/article/59b09322c90344f5b94a3ad3adf684d82017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09070-yhttps://doaj.org/toc/2045-2322Abstract Various techniques have been developed to study changes in the cerebral vasculature in numerous neuropathological processes including subarachnoid hemorrhage (SAH). One of the most widely employed techniques uses India ink-gelatin casting, which presents numerous challenges due to its high viscosity, rapid solidification, and its impact on immunohistochemical analysis. To overcome these limitations, we developed a novel technique for assessing cerebral vasospasm using cerebrovascular perfusion with ROX, SE (5-Carboxy-X-Rhodamine, Succinimidyl Ester), a fluorescent labeling dye. We found that ROX SE perfusion achieves excellent delineation of the cerebral vasculature, was qualitatively and quantitatively superior to India ink-gelatin casting for the assessment of cerebral vasospasm, permits outstanding immunohistochemical examination of non-vasospasm components of secondary brain injury, and is a more efficient and cost-effective experimental technique. ROX SE perfusion is therefore a novel and highly useful technique for studying cerebrovascular pathology following experimental SAH.Diane J. AumAnanth K. VellimanaItender SinghEric MilnerJames W. NelsonByung Hee HanGregory J. ZipfelNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Diane J. Aum Ananth K. Vellimana Itender Singh Eric Milner James W. Nelson Byung Hee Han Gregory J. Zipfel A novel fluorescent imaging technique for assessment of cerebral vasospasm after experimental subarachnoid hemorrhage
description	Abstract Various techniques have been developed to study changes in the cerebral vasculature in numerous neuropathological processes including subarachnoid hemorrhage (SAH). One of the most widely employed techniques uses India ink-gelatin casting, which presents numerous challenges due to its high viscosity, rapid solidification, and its impact on immunohistochemical analysis. To overcome these limitations, we developed a novel technique for assessing cerebral vasospasm using cerebrovascular perfusion with ROX, SE (5-Carboxy-X-Rhodamine, Succinimidyl Ester), a fluorescent labeling dye. We found that ROX SE perfusion achieves excellent delineation of the cerebral vasculature, was qualitatively and quantitatively superior to India ink-gelatin casting for the assessment of cerebral vasospasm, permits outstanding immunohistochemical examination of non-vasospasm components of secondary brain injury, and is a more efficient and cost-effective experimental technique. ROX SE perfusion is therefore a novel and highly useful technique for studying cerebrovascular pathology following experimental SAH.
format	article
author	Diane J. Aum Ananth K. Vellimana Itender Singh Eric Milner James W. Nelson Byung Hee Han Gregory J. Zipfel
author_facet	Diane J. Aum Ananth K. Vellimana Itender Singh Eric Milner James W. Nelson Byung Hee Han Gregory J. Zipfel
author_sort	Diane J. Aum
title	A novel fluorescent imaging technique for assessment of cerebral vasospasm after experimental subarachnoid hemorrhage
title_short	A novel fluorescent imaging technique for assessment of cerebral vasospasm after experimental subarachnoid hemorrhage
title_full	A novel fluorescent imaging technique for assessment of cerebral vasospasm after experimental subarachnoid hemorrhage
title_fullStr	A novel fluorescent imaging technique for assessment of cerebral vasospasm after experimental subarachnoid hemorrhage
title_full_unstemmed	A novel fluorescent imaging technique for assessment of cerebral vasospasm after experimental subarachnoid hemorrhage
title_sort	novel fluorescent imaging technique for assessment of cerebral vasospasm after experimental subarachnoid hemorrhage
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/59b09322c90344f5b94a3ad3adf684d8
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A novel fluorescent imaging technique for assessment of cerebral vasospasm after experimental subarachnoid hemorrhage

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