Characterization of Penaeus vannamei mitogenome focusing on genetic diversity.

The diversity of the Penaeus vannamei mitochondrial genome has still been poorly characterized, there are no validated mitochondrial markers available for populational studies, and the heteroplasmy has not yet been investigated in this species. In this study, metagenomic reads extracted from the mus...

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Autores principales:	Paulo Eduardo T Soares, Márcia Danielle A Dantas, Rita de Cássia B Silva-Portela, Lucymara F Agnez-Lima, Daniel Carlos F Lanza
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/59b3fd30f4ad4c5c9c40f6d5a1d891ea
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spelling	oai:doaj.org-article:59b3fd30f4ad4c5c9c40f6d5a1d891ea2021-12-02T20:08:48ZCharacterization of Penaeus vannamei mitogenome focusing on genetic diversity.1932-620310.1371/journal.pone.0255291https://doaj.org/article/59b3fd30f4ad4c5c9c40f6d5a1d891ea2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255291https://doaj.org/toc/1932-6203The diversity of the Penaeus vannamei mitochondrial genome has still been poorly characterized, there are no validated mitochondrial markers available for populational studies, and the heteroplasmy has not yet been investigated in this species. In this study, metagenomic reads extracted from the muscle of a single individual were used to assemble the mitochondrial genome (mtDNA). These data associated with mitochondrial genomes previously described allowed to evaluate the inter-individual variability and heteroplasmy. Comparison among 45 mtDNA control regions led to the detection of conserved and variable segments and the characterization of two hypervariable regions. The analysis of diversity revealed mostly low frequency polymorphisms, and heteroplasmy was found in practically all mitochondrial genes, with a high occurrence of indels. These results indicate that the design of mitochondrial markers for P. vannamei must be done with caution. The mapping of conserved and variable regions and the characterization of heteroplasmy presented here will contribute to increasing the efficiency of mitochondrial markers for population or individual studies.Paulo Eduardo T SoaresMárcia Danielle A DantasRita de Cássia B Silva-PortelaLucymara F Agnez-LimaDaniel Carlos F LanzaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0255291 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Paulo Eduardo T Soares Márcia Danielle A Dantas Rita de Cássia B Silva-Portela Lucymara F Agnez-Lima Daniel Carlos F Lanza Characterization of Penaeus vannamei mitogenome focusing on genetic diversity.
description	The diversity of the Penaeus vannamei mitochondrial genome has still been poorly characterized, there are no validated mitochondrial markers available for populational studies, and the heteroplasmy has not yet been investigated in this species. In this study, metagenomic reads extracted from the muscle of a single individual were used to assemble the mitochondrial genome (mtDNA). These data associated with mitochondrial genomes previously described allowed to evaluate the inter-individual variability and heteroplasmy. Comparison among 45 mtDNA control regions led to the detection of conserved and variable segments and the characterization of two hypervariable regions. The analysis of diversity revealed mostly low frequency polymorphisms, and heteroplasmy was found in practically all mitochondrial genes, with a high occurrence of indels. These results indicate that the design of mitochondrial markers for P. vannamei must be done with caution. The mapping of conserved and variable regions and the characterization of heteroplasmy presented here will contribute to increasing the efficiency of mitochondrial markers for population or individual studies.
format	article
author	Paulo Eduardo T Soares Márcia Danielle A Dantas Rita de Cássia B Silva-Portela Lucymara F Agnez-Lima Daniel Carlos F Lanza
author_facet	Paulo Eduardo T Soares Márcia Danielle A Dantas Rita de Cássia B Silva-Portela Lucymara F Agnez-Lima Daniel Carlos F Lanza
author_sort	Paulo Eduardo T Soares
title	Characterization of Penaeus vannamei mitogenome focusing on genetic diversity.
title_short	Characterization of Penaeus vannamei mitogenome focusing on genetic diversity.
title_full	Characterization of Penaeus vannamei mitogenome focusing on genetic diversity.
title_fullStr	Characterization of Penaeus vannamei mitogenome focusing on genetic diversity.
title_full_unstemmed	Characterization of Penaeus vannamei mitogenome focusing on genetic diversity.
title_sort	characterization of penaeus vannamei mitogenome focusing on genetic diversity.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/59b3fd30f4ad4c5c9c40f6d5a1d891ea
work_keys_str_mv	AT pauloeduardotsoares characterizationofpenaeusvannameimitogenomefocusingongeneticdiversity AT marciadanielleadantas characterizationofpenaeusvannameimitogenomefocusingongeneticdiversity AT ritadecassiabsilvaportela characterizationofpenaeusvannameimitogenomefocusingongeneticdiversity AT lucymarafagnezlima characterizationofpenaeusvannameimitogenomefocusingongeneticdiversity AT danielcarlosflanza characterizationofpenaeusvannameimitogenomefocusingongeneticdiversity
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Characterization of Penaeus vannamei mitogenome focusing on genetic diversity.

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