Functional analysis of enhancer elements regulating the expression of the Drosophila homeodomain transcription factor DRx by gene targeting

Abstract Background The Drosophila brain is an ideal model system to study stem cells, here called neuroblasts, and the generation of neural lineages. Many transcriptional activators are involved in formation of the brain during the development of Drosophila melanogaster. The transcription factor Dr...

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Autores principales:	Christine Klöppel, Kirsten Hildebrandt, Dieter Kolb, Nora Fürst, Isabelle Bley, Ruth-Jessica Karlowatz, Uwe Walldorf
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Drosophila retinal homeobox (DRx) Transcription factor Enhancer Gene targeting Genetics QH426-470
Acceso en línea:	https://doaj.org/article/59c442436e8048bdae0ea354b0cadd89
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spelling	oai:doaj.org-article:59c442436e8048bdae0ea354b0cadd892021-11-07T12:15:26ZFunctional analysis of enhancer elements regulating the expression of the Drosophila homeodomain transcription factor DRx by gene targeting10.1186/s41065-021-00210-z1601-5223https://doaj.org/article/59c442436e8048bdae0ea354b0cadd892021-11-01T00:00:00Zhttps://doi.org/10.1186/s41065-021-00210-zhttps://doaj.org/toc/1601-5223Abstract Background The Drosophila brain is an ideal model system to study stem cells, here called neuroblasts, and the generation of neural lineages. Many transcriptional activators are involved in formation of the brain during the development of Drosophila melanogaster. The transcription factor Drosophila Retinal homeobox (DRx), a member of the 57B homeobox gene cluster, is also one of these factors for brain development. Results In this study a detailed expression analysis of DRx in different developmental stages was conducted. We show that DRx is expressed in the embryonic brain in the protocerebrum, in the larval brain in the DM and DL lineages, the medulla and the lobula complex and in the central complex of the adult brain. We generated a DRx enhancer trap strain by gene targeting and reintegration of Gal4, which mimics the endogenous expression of DRx. With the help of eight existing enhancer-Gal4 strains and one made by our group, we mapped various enhancers necessary for the expression of DRx during all stages of brain development from the embryo to the adult. We made an analysis of some larger enhancer regions by gene targeting. Deletion of three of these enhancers showing the most prominent expression patterns in the brain resulted in specific temporal and spatial loss of DRx expression in defined brain structures. Conclusion Our data show that DRx is expressed in specific neuroblasts and defined neural lineages and suggest that DRx is another important factor for Drosophila brain development.Christine KlöppelKirsten HildebrandtDieter KolbNora FürstIsabelle BleyRuth-Jessica KarlowatzUwe WalldorfBMCarticleDrosophila retinal homeobox (DRx)Transcription factorEnhancerGene targetingGeneticsQH426-470ENHereditas, Vol 158, Iss 1, Pp 1-20 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Drosophila retinal homeobox (DRx) Transcription factor Enhancer Gene targeting Genetics QH426-470
spellingShingle	Drosophila retinal homeobox (DRx) Transcription factor Enhancer Gene targeting Genetics QH426-470 Christine Klöppel Kirsten Hildebrandt Dieter Kolb Nora Fürst Isabelle Bley Ruth-Jessica Karlowatz Uwe Walldorf Functional analysis of enhancer elements regulating the expression of the Drosophila homeodomain transcription factor DRx by gene targeting
description	Abstract Background The Drosophila brain is an ideal model system to study stem cells, here called neuroblasts, and the generation of neural lineages. Many transcriptional activators are involved in formation of the brain during the development of Drosophila melanogaster. The transcription factor Drosophila Retinal homeobox (DRx), a member of the 57B homeobox gene cluster, is also one of these factors for brain development. Results In this study a detailed expression analysis of DRx in different developmental stages was conducted. We show that DRx is expressed in the embryonic brain in the protocerebrum, in the larval brain in the DM and DL lineages, the medulla and the lobula complex and in the central complex of the adult brain. We generated a DRx enhancer trap strain by gene targeting and reintegration of Gal4, which mimics the endogenous expression of DRx. With the help of eight existing enhancer-Gal4 strains and one made by our group, we mapped various enhancers necessary for the expression of DRx during all stages of brain development from the embryo to the adult. We made an analysis of some larger enhancer regions by gene targeting. Deletion of three of these enhancers showing the most prominent expression patterns in the brain resulted in specific temporal and spatial loss of DRx expression in defined brain structures. Conclusion Our data show that DRx is expressed in specific neuroblasts and defined neural lineages and suggest that DRx is another important factor for Drosophila brain development.
format	article
author	Christine Klöppel Kirsten Hildebrandt Dieter Kolb Nora Fürst Isabelle Bley Ruth-Jessica Karlowatz Uwe Walldorf
author_facet	Christine Klöppel Kirsten Hildebrandt Dieter Kolb Nora Fürst Isabelle Bley Ruth-Jessica Karlowatz Uwe Walldorf
author_sort	Christine Klöppel
title	Functional analysis of enhancer elements regulating the expression of the Drosophila homeodomain transcription factor DRx by gene targeting
title_short	Functional analysis of enhancer elements regulating the expression of the Drosophila homeodomain transcription factor DRx by gene targeting
title_full	Functional analysis of enhancer elements regulating the expression of the Drosophila homeodomain transcription factor DRx by gene targeting
title_fullStr	Functional analysis of enhancer elements regulating the expression of the Drosophila homeodomain transcription factor DRx by gene targeting
title_full_unstemmed	Functional analysis of enhancer elements regulating the expression of the Drosophila homeodomain transcription factor DRx by gene targeting
title_sort	functional analysis of enhancer elements regulating the expression of the drosophila homeodomain transcription factor drx by gene targeting
publisher	BMC
publishDate	2021
url	https://doaj.org/article/59c442436e8048bdae0ea354b0cadd89
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Functional analysis of enhancer elements regulating the expression of the Drosophila homeodomain transcription factor DRx by gene targeting

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