Peptide-guided JC polyomavirus-like particles specifically target bladder cancer cells for gene therapy

Abstract The ultimate goal of gene delivery vectors is to establish specific and effective treatments for human diseases. We previously demonstrated that human JC polyomavirus (JCPyV) virus-like particles (VLPs) can package and deliver exogenous DNA into susceptible cells for gene expression. For ti...

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Autores principales:	Wei-Hong Lai, Chiung-Yao Fang, Ming-Chieh Chou, Mien-Chun Lin, Cheng-Huang Shen, Chun-Nun Chao, Yeong‐Chin Jou, Deching Chang, Meilin Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/59cd74bb6c5149f8a96541e47988ae7e
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spelling	oai:doaj.org-article:59cd74bb6c5149f8a96541e47988ae7e2021-12-02T15:03:05ZPeptide-guided JC polyomavirus-like particles specifically target bladder cancer cells for gene therapy10.1038/s41598-021-91328-72045-2322https://doaj.org/article/59cd74bb6c5149f8a96541e47988ae7e2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91328-7https://doaj.org/toc/2045-2322Abstract The ultimate goal of gene delivery vectors is to establish specific and effective treatments for human diseases. We previously demonstrated that human JC polyomavirus (JCPyV) virus-like particles (VLPs) can package and deliver exogenous DNA into susceptible cells for gene expression. For tissue-specific targeting in this study, JCPyV VLPs were conjugated with a specific peptide for bladder cancer (SPB) that specifically binds to bladder cancer cells. The suicide gene thymidine kinase was packaged and delivered by SPB-conjugated VLPs (VLP-SPBs). Expression of the suicide gene was detected only in human bladder cancer cells and not in lung cancer or neuroblastoma cells susceptible to JCPyV VLP infection in vitro and in vivo, demonstrating the target specificity of VLP-SPBs. The gene transduction efficiency of VLP-SPBs was approximately 100 times greater than that of VLPs without the conjugated peptide. JCPyV VLPs can be specifically guided to target particular cell types when tagged with a ligand molecule that binds to a cell surface marker, thereby improving gene therapy.Wei-Hong LaiChiung-Yao FangMing-Chieh ChouMien-Chun LinCheng-Huang ShenChun-Nun ChaoYeong‐Chin JouDeching ChangMeilin WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wei-Hong Lai Chiung-Yao Fang Ming-Chieh Chou Mien-Chun Lin Cheng-Huang Shen Chun-Nun Chao Yeong‐Chin Jou Deching Chang Meilin Wang Peptide-guided JC polyomavirus-like particles specifically target bladder cancer cells for gene therapy
description	Abstract The ultimate goal of gene delivery vectors is to establish specific and effective treatments for human diseases. We previously demonstrated that human JC polyomavirus (JCPyV) virus-like particles (VLPs) can package and deliver exogenous DNA into susceptible cells for gene expression. For tissue-specific targeting in this study, JCPyV VLPs were conjugated with a specific peptide for bladder cancer (SPB) that specifically binds to bladder cancer cells. The suicide gene thymidine kinase was packaged and delivered by SPB-conjugated VLPs (VLP-SPBs). Expression of the suicide gene was detected only in human bladder cancer cells and not in lung cancer or neuroblastoma cells susceptible to JCPyV VLP infection in vitro and in vivo, demonstrating the target specificity of VLP-SPBs. The gene transduction efficiency of VLP-SPBs was approximately 100 times greater than that of VLPs without the conjugated peptide. JCPyV VLPs can be specifically guided to target particular cell types when tagged with a ligand molecule that binds to a cell surface marker, thereby improving gene therapy.
format	article
author	Wei-Hong Lai Chiung-Yao Fang Ming-Chieh Chou Mien-Chun Lin Cheng-Huang Shen Chun-Nun Chao Yeong‐Chin Jou Deching Chang Meilin Wang
author_facet	Wei-Hong Lai Chiung-Yao Fang Ming-Chieh Chou Mien-Chun Lin Cheng-Huang Shen Chun-Nun Chao Yeong‐Chin Jou Deching Chang Meilin Wang
author_sort	Wei-Hong Lai
title	Peptide-guided JC polyomavirus-like particles specifically target bladder cancer cells for gene therapy
title_short	Peptide-guided JC polyomavirus-like particles specifically target bladder cancer cells for gene therapy
title_full	Peptide-guided JC polyomavirus-like particles specifically target bladder cancer cells for gene therapy
title_fullStr	Peptide-guided JC polyomavirus-like particles specifically target bladder cancer cells for gene therapy
title_full_unstemmed	Peptide-guided JC polyomavirus-like particles specifically target bladder cancer cells for gene therapy
title_sort	peptide-guided jc polyomavirus-like particles specifically target bladder cancer cells for gene therapy
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/59cd74bb6c5149f8a96541e47988ae7e
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Peptide-guided JC polyomavirus-like particles specifically target bladder cancer cells for gene therapy

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