M-MuLV reverse transcriptase: Selected properties and improved mutants

Reverse transcriptases (RTs) are enzymes synthesizing DNA using RNA as the template and serving as the standard tools in modern biotechnology and molecular diagnostics. To date, the most commonly used reverse transcriptase is the enzyme from Moloney murine leukemia virus, M-MuLV RT. Since its discov...

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Autores principales:	Igor P. Oscorbin, Maxim L. Filipenko
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	M-MuLV RT Reverse transcriptase Site-directed mutagenesis Random mutagenesis Fusion proteins Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/59d4d43919f34c0eb46fdb5f354b994d
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spelling	oai:doaj.org-article:59d4d43919f34c0eb46fdb5f354b994d2021-12-02T05:00:25ZM-MuLV reverse transcriptase: Selected properties and improved mutants2001-037010.1016/j.csbj.2021.11.030https://doaj.org/article/59d4d43919f34c0eb46fdb5f354b994d2021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2001037021004955https://doaj.org/toc/2001-0370Reverse transcriptases (RTs) are enzymes synthesizing DNA using RNA as the template and serving as the standard tools in modern biotechnology and molecular diagnostics. To date, the most commonly used reverse transcriptase is the enzyme from Moloney murine leukemia virus, M-MuLV RT. Since its discovery, M-MuLV RT has become indispensable for modern RNA studies; the range of M-MuLV RT applications is vast, from scientific tasks to clinical testing of human pathogens. This review will give a brief description of the structure, thermal stability, processivity, and fidelity, focusing on improving M-MuLV RT for practical usage.Igor P. OscorbinMaxim L. FilipenkoElsevierarticleM-MuLV RTReverse transcriptaseSite-directed mutagenesisRandom mutagenesisFusion proteinsBiotechnologyTP248.13-248.65ENComputational and Structural Biotechnology Journal, Vol 19, Iss , Pp 6315-6327 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	M-MuLV RT Reverse transcriptase Site-directed mutagenesis Random mutagenesis Fusion proteins Biotechnology TP248.13-248.65
spellingShingle	M-MuLV RT Reverse transcriptase Site-directed mutagenesis Random mutagenesis Fusion proteins Biotechnology TP248.13-248.65 Igor P. Oscorbin Maxim L. Filipenko M-MuLV reverse transcriptase: Selected properties and improved mutants
description	Reverse transcriptases (RTs) are enzymes synthesizing DNA using RNA as the template and serving as the standard tools in modern biotechnology and molecular diagnostics. To date, the most commonly used reverse transcriptase is the enzyme from Moloney murine leukemia virus, M-MuLV RT. Since its discovery, M-MuLV RT has become indispensable for modern RNA studies; the range of M-MuLV RT applications is vast, from scientific tasks to clinical testing of human pathogens. This review will give a brief description of the structure, thermal stability, processivity, and fidelity, focusing on improving M-MuLV RT for practical usage.
format	article
author	Igor P. Oscorbin Maxim L. Filipenko
author_facet	Igor P. Oscorbin Maxim L. Filipenko
author_sort	Igor P. Oscorbin
title	M-MuLV reverse transcriptase: Selected properties and improved mutants
title_short	M-MuLV reverse transcriptase: Selected properties and improved mutants
title_full	M-MuLV reverse transcriptase: Selected properties and improved mutants
title_fullStr	M-MuLV reverse transcriptase: Selected properties and improved mutants
title_full_unstemmed	M-MuLV reverse transcriptase: Selected properties and improved mutants
title_sort	m-mulv reverse transcriptase: selected properties and improved mutants
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/59d4d43919f34c0eb46fdb5f354b994d
work_keys_str_mv	AT igorposcorbin mmulvreversetranscriptaseselectedpropertiesandimprovedmutants AT maximlfilipenko mmulvreversetranscriptaseselectedpropertiesandimprovedmutants
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M-MuLV reverse transcriptase: Selected properties and improved mutants

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