Alteration of complement hemolytic activity in different trauma and sepsis models

Christian Ehrnthaller,1 Umme Amara,1 Sebastian Weckbach,1 Miriam Kalbitz,1 Markus Huber-Lang,1 Soheyl Bahrami21Department of Traumatology, Hand, Plastic, and Reconstructive Surgery, Center of Surgery, University of Ulm, Germany; 2Ludwig Boltzmann Institute for Experimental and Clinical Traumatology,...

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Autores principales:	Ehrnthaller C, Amara U, Weckbach S, Kalbitz M, Huber-Lang M, Bahrami S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/59d5545deedf4656bb2db3b57c0213fa
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spelling	oai:doaj.org-article:59d5545deedf4656bb2db3b57c0213fa2021-12-02T01:25:33ZAlteration of complement hemolytic activity in different trauma and sepsis models1178-7031https://doaj.org/article/59d5545deedf4656bb2db3b57c0213fa2012-07-01T00:00:00Zhttp://www.dovepress.com/alteration-of-complement-hemolytic-activity-in-different-trauma-and-se-a10540https://doaj.org/toc/1178-7031Christian Ehrnthaller,1 Umme Amara,1 Sebastian Weckbach,1 Miriam Kalbitz,1 Markus Huber-Lang,1 Soheyl Bahrami21Department of Traumatology, Hand, Plastic, and Reconstructive Surgery, Center of Surgery, University of Ulm, Germany; 2Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, AustriaAbstract: Complement activation is involved in various diseases in which innate immunity plays a crucial role. However, its pathophysiological relevance is not clearly understood. Experimental models have been widely used to characterize the role of complement activation under different pathological conditions, such as hypoxemia, ischemia and reperfusion, tissue damage, and polymicrobial invasion. Screening of the complement status and function is, however, strongly dependent on the laboratory-specific techniques being used to sample and measure complement, making it difficult to compare the results found in different laboratories. Therefore, we evaluated complement function by measuring complement hemolytic activity (CH50) in various animal models of isolated ischemia reperfusion (I/R: kidney, liver, gut), hemorrhagic traumatic shock (HTS), endotoxic shock (LPS), and sepsis (CLP). Complement activation was less pronounced in isolated models of ischemia and reperfusion, whereas a strong complement response was observed early after HTS, CLP, and LPS. In summary, CH50 is a well-established, quick, and cost-effective screening method of complement function. However, because we obtained different results in clinically relevant animal models, further differentiation using specific complement factor analysis is necessary.Keywords: CH50, complement, hemorrhagic shock, inflammation, ischemia/reperfusion, sepsisEhrnthaller CAmara UWeckbach SKalbitz MHuber-Lang MBahrami SDove Medical PressarticlePathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol 2012, Iss default, Pp 59-66 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Ehrnthaller C Amara U Weckbach S Kalbitz M Huber-Lang M Bahrami S Alteration of complement hemolytic activity in different trauma and sepsis models
description	Christian Ehrnthaller,1 Umme Amara,1 Sebastian Weckbach,1 Miriam Kalbitz,1 Markus Huber-Lang,1 Soheyl Bahrami21Department of Traumatology, Hand, Plastic, and Reconstructive Surgery, Center of Surgery, University of Ulm, Germany; 2Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, AustriaAbstract: Complement activation is involved in various diseases in which innate immunity plays a crucial role. However, its pathophysiological relevance is not clearly understood. Experimental models have been widely used to characterize the role of complement activation under different pathological conditions, such as hypoxemia, ischemia and reperfusion, tissue damage, and polymicrobial invasion. Screening of the complement status and function is, however, strongly dependent on the laboratory-specific techniques being used to sample and measure complement, making it difficult to compare the results found in different laboratories. Therefore, we evaluated complement function by measuring complement hemolytic activity (CH50) in various animal models of isolated ischemia reperfusion (I/R: kidney, liver, gut), hemorrhagic traumatic shock (HTS), endotoxic shock (LPS), and sepsis (CLP). Complement activation was less pronounced in isolated models of ischemia and reperfusion, whereas a strong complement response was observed early after HTS, CLP, and LPS. In summary, CH50 is a well-established, quick, and cost-effective screening method of complement function. However, because we obtained different results in clinically relevant animal models, further differentiation using specific complement factor analysis is necessary.Keywords: CH50, complement, hemorrhagic shock, inflammation, ischemia/reperfusion, sepsis
format	article
author	Ehrnthaller C Amara U Weckbach S Kalbitz M Huber-Lang M Bahrami S
author_facet	Ehrnthaller C Amara U Weckbach S Kalbitz M Huber-Lang M Bahrami S
author_sort	Ehrnthaller C
title	Alteration of complement hemolytic activity in different trauma and sepsis models
title_short	Alteration of complement hemolytic activity in different trauma and sepsis models
title_full	Alteration of complement hemolytic activity in different trauma and sepsis models
title_fullStr	Alteration of complement hemolytic activity in different trauma and sepsis models
title_full_unstemmed	Alteration of complement hemolytic activity in different trauma and sepsis models
title_sort	alteration of complement hemolytic activity in different trauma and sepsis models
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/59d5545deedf4656bb2db3b57c0213fa
work_keys_str_mv	AT ehrnthallerc alterationofcomplementhemolyticactivityindifferenttraumaandsepsismodels AT amarau alterationofcomplementhemolyticactivityindifferenttraumaandsepsismodels AT weckbachs alterationofcomplementhemolyticactivityindifferenttraumaandsepsismodels AT kalbitzm alterationofcomplementhemolyticactivityindifferenttraumaandsepsismodels AT huberlangm alterationofcomplementhemolyticactivityindifferenttraumaandsepsismodels AT bahramis alterationofcomplementhemolyticactivityindifferenttraumaandsepsismodels
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Alteration of complement hemolytic activity in different trauma and sepsis models

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