Optimized somatic embryogenesis and plant regeneration in elite Argentinian sugarcane (Saccharum spp.) cultivars

Abstract Background Biotechnological breeding of elite sugarcane cultivars is currently limited because of the difficulty of regenerating plants by tissue culture. Here, we report that commercially elite sugarcane genotypes, which are adapted to Argentinian agro-ecological conditions, are capable of...

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Autores principales:	Valentina Di Pauli, Paola Daniela Fontana, Dalia Marcela Lewi, Arturo Felipe, Luis Ernesto Erazzú
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Biotechnological breeding Tissue culture Callus Somatic embryos Genotype dependence Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/59d599cd59e348ef9c1de4e99860e655
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spelling	oai:doaj.org-article:59d599cd59e348ef9c1de4e99860e6552021-11-14T12:32:55ZOptimized somatic embryogenesis and plant regeneration in elite Argentinian sugarcane (Saccharum spp.) cultivars10.1186/s43141-021-00270-82090-5920https://doaj.org/article/59d599cd59e348ef9c1de4e99860e6552021-11-01T00:00:00Zhttps://doi.org/10.1186/s43141-021-00270-8https://doaj.org/toc/2090-5920Abstract Background Biotechnological breeding of elite sugarcane cultivars is currently limited because of the difficulty of regenerating plants by tissue culture. Here, we report that commercially elite sugarcane genotypes, which are adapted to Argentinian agro-ecological conditions, are capable of being regenerated via indirect somatic embryogenesis. Leaf rolls of five elite genotypes were cultured following two callus induction protocols using different concentrations of 2,4-D as the growth regulator. Embryogenic calluses were regenerated under light conditions. Regenerated plants were subsequently acclimatized in the greenhouse under two acclimatization procedures before being transplanted to the field. Results Four of the five genotypes were able to form somatic embryos following the two induction protocols. The variables related to embryogenic callus production were influenced by the interaction between genotype and culture conditions. For plant regeneration, the embryogenic calluses were further cultured on an IBA-supplemented medium, where we observed a high genotype dependence. Calluses from the four cultivars regenerated a good number of plants. With the procedures described here, we obtained more than 90% of well-acclimatized plants both in the greenhouse and in the field. Conclusions This protocol provides a simple way to regenerate sugarcane plants through indirect somatic embryogenesis. Also, the results confirm that tissue culture ability is highly genotype-dependent in sugarcane. Our findings suggest that these elite cultivars could be good candidates for biotechnological breeding.Valentina Di PauliPaola Daniela FontanaDalia Marcela LewiArturo FelipeLuis Ernesto ErazzúSpringerOpenarticleBiotechnological breedingTissue cultureCallusSomatic embryosGenotype dependenceBiotechnologyTP248.13-248.65GeneticsQH426-470ENJournal of Genetic Engineering and Biotechnology, Vol 19, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biotechnological breeding Tissue culture Callus Somatic embryos Genotype dependence Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	Biotechnological breeding Tissue culture Callus Somatic embryos Genotype dependence Biotechnology TP248.13-248.65 Genetics QH426-470 Valentina Di Pauli Paola Daniela Fontana Dalia Marcela Lewi Arturo Felipe Luis Ernesto Erazzú Optimized somatic embryogenesis and plant regeneration in elite Argentinian sugarcane (Saccharum spp.) cultivars
description	Abstract Background Biotechnological breeding of elite sugarcane cultivars is currently limited because of the difficulty of regenerating plants by tissue culture. Here, we report that commercially elite sugarcane genotypes, which are adapted to Argentinian agro-ecological conditions, are capable of being regenerated via indirect somatic embryogenesis. Leaf rolls of five elite genotypes were cultured following two callus induction protocols using different concentrations of 2,4-D as the growth regulator. Embryogenic calluses were regenerated under light conditions. Regenerated plants were subsequently acclimatized in the greenhouse under two acclimatization procedures before being transplanted to the field. Results Four of the five genotypes were able to form somatic embryos following the two induction protocols. The variables related to embryogenic callus production were influenced by the interaction between genotype and culture conditions. For plant regeneration, the embryogenic calluses were further cultured on an IBA-supplemented medium, where we observed a high genotype dependence. Calluses from the four cultivars regenerated a good number of plants. With the procedures described here, we obtained more than 90% of well-acclimatized plants both in the greenhouse and in the field. Conclusions This protocol provides a simple way to regenerate sugarcane plants through indirect somatic embryogenesis. Also, the results confirm that tissue culture ability is highly genotype-dependent in sugarcane. Our findings suggest that these elite cultivars could be good candidates for biotechnological breeding.
format	article
author	Valentina Di Pauli Paola Daniela Fontana Dalia Marcela Lewi Arturo Felipe Luis Ernesto Erazzú
author_facet	Valentina Di Pauli Paola Daniela Fontana Dalia Marcela Lewi Arturo Felipe Luis Ernesto Erazzú
author_sort	Valentina Di Pauli
title	Optimized somatic embryogenesis and plant regeneration in elite Argentinian sugarcane (Saccharum spp.) cultivars
title_short	Optimized somatic embryogenesis and plant regeneration in elite Argentinian sugarcane (Saccharum spp.) cultivars
title_full	Optimized somatic embryogenesis and plant regeneration in elite Argentinian sugarcane (Saccharum spp.) cultivars
title_fullStr	Optimized somatic embryogenesis and plant regeneration in elite Argentinian sugarcane (Saccharum spp.) cultivars
title_full_unstemmed	Optimized somatic embryogenesis and plant regeneration in elite Argentinian sugarcane (Saccharum spp.) cultivars
title_sort	optimized somatic embryogenesis and plant regeneration in elite argentinian sugarcane (saccharum spp.) cultivars
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/59d599cd59e348ef9c1de4e99860e655
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Optimized somatic embryogenesis and plant regeneration in elite Argentinian sugarcane (Saccharum spp.) cultivars

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