miRNAs expression analysis in paired fresh/frozen and dissected formalin fixed and paraffin embedded glioblastoma using real-time pCR.

miRNAs are small molecules involved in gene regulation. Each tissue shows a characteristic miRNAs epression profile that could be altered during neoplastic transformation. Glioblastoma is the most aggressive brain tumour of the adult with a high rate of mortality. Recognizing a specific pattern of m...

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Autores principales:	Dario de Biase, Michela Visani, Luca Morandi, Gianluca Marucci, Cristian Taccioli, Serenella Cerasoli, Agostino Baruzzi, Annalisa Pession, PERNO Study Group
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/59d658bd515f497d9c47982a59398ed1
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spelling	oai:doaj.org-article:59d658bd515f497d9c47982a59398ed12021-11-18T07:21:41ZmiRNAs expression analysis in paired fresh/frozen and dissected formalin fixed and paraffin embedded glioblastoma using real-time pCR.1932-620310.1371/journal.pone.0035596https://doaj.org/article/59d658bd515f497d9c47982a59398ed12012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22530056/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203miRNAs are small molecules involved in gene regulation. Each tissue shows a characteristic miRNAs epression profile that could be altered during neoplastic transformation. Glioblastoma is the most aggressive brain tumour of the adult with a high rate of mortality. Recognizing a specific pattern of miRNAs for GBM could provide further boost for target therapy. The availability of fresh tissue for brain specimens is often limited and for this reason the possibility of starting from formalin fixed and paraffin embedded tissue (FFPE) could very helpful even in miRNAs expression analysis. We analysed a panel of 19 miRNAs in 30 paired samples starting both from FFPE and Fresh/Frozen material. Our data revealed that there is a good correlation in results obtained from FFPE in comparison with those obtained analysing miRNAs extracted from Fresh/Frozen specimen. In the few cases with a not good correlation value we noticed that the discrepancy could be due to dissection performed in FFPE samples. To the best of our knowledge this is the first paper demonstrating that the results obtained in miRNAs analysis using Real-Time PCR starting from FFPE specimens of glioblastoma are comparable with those obtained in Fresh/Frozen samples.Dario de BiaseMichela VisaniLuca MorandiGianluca MarucciCristian TaccioliSerenella CerasoliAgostino BaruzziAnnalisa PessionPERNO Study GroupPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e35596 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dario de Biase Michela Visani Luca Morandi Gianluca Marucci Cristian Taccioli Serenella Cerasoli Agostino Baruzzi Annalisa Pession PERNO Study Group miRNAs expression analysis in paired fresh/frozen and dissected formalin fixed and paraffin embedded glioblastoma using real-time pCR.
description	miRNAs are small molecules involved in gene regulation. Each tissue shows a characteristic miRNAs epression profile that could be altered during neoplastic transformation. Glioblastoma is the most aggressive brain tumour of the adult with a high rate of mortality. Recognizing a specific pattern of miRNAs for GBM could provide further boost for target therapy. The availability of fresh tissue for brain specimens is often limited and for this reason the possibility of starting from formalin fixed and paraffin embedded tissue (FFPE) could very helpful even in miRNAs expression analysis. We analysed a panel of 19 miRNAs in 30 paired samples starting both from FFPE and Fresh/Frozen material. Our data revealed that there is a good correlation in results obtained from FFPE in comparison with those obtained analysing miRNAs extracted from Fresh/Frozen specimen. In the few cases with a not good correlation value we noticed that the discrepancy could be due to dissection performed in FFPE samples. To the best of our knowledge this is the first paper demonstrating that the results obtained in miRNAs analysis using Real-Time PCR starting from FFPE specimens of glioblastoma are comparable with those obtained in Fresh/Frozen samples.
format	article
author	Dario de Biase Michela Visani Luca Morandi Gianluca Marucci Cristian Taccioli Serenella Cerasoli Agostino Baruzzi Annalisa Pession PERNO Study Group
author_facet	Dario de Biase Michela Visani Luca Morandi Gianluca Marucci Cristian Taccioli Serenella Cerasoli Agostino Baruzzi Annalisa Pession PERNO Study Group
author_sort	Dario de Biase
title	miRNAs expression analysis in paired fresh/frozen and dissected formalin fixed and paraffin embedded glioblastoma using real-time pCR.
title_short	miRNAs expression analysis in paired fresh/frozen and dissected formalin fixed and paraffin embedded glioblastoma using real-time pCR.
title_full	miRNAs expression analysis in paired fresh/frozen and dissected formalin fixed and paraffin embedded glioblastoma using real-time pCR.
title_fullStr	miRNAs expression analysis in paired fresh/frozen and dissected formalin fixed and paraffin embedded glioblastoma using real-time pCR.
title_full_unstemmed	miRNAs expression analysis in paired fresh/frozen and dissected formalin fixed and paraffin embedded glioblastoma using real-time pCR.
title_sort	mirnas expression analysis in paired fresh/frozen and dissected formalin fixed and paraffin embedded glioblastoma using real-time pcr.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/59d658bd515f497d9c47982a59398ed1
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miRNAs expression analysis in paired fresh/frozen and dissected formalin fixed and paraffin embedded glioblastoma using real-time pCR.

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