Dereplication of antimicrobial biosurfactants from marine bacteria using molecular networking

Abstract Biosurfactants are amphiphilic surface-active molecules of microbial origin principally produced by hydrocarbon-degrading bacteria; in addition to the bioremediation properties, they can also present antimicrobial activity. The present study highlights the chemical characterization and the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Albert D. Patiño, Manuela Montoya-Giraldo, Marynes Quintero, Lizbeth L. López-Parra, Lina M. Blandón, Javier Gómez-León
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/59d6c9e4ca76453689eebb0819152418
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:59d6c9e4ca76453689eebb0819152418
record_format	dspace
spelling	oai:doaj.org-article:59d6c9e4ca76453689eebb08191524182021-12-02T16:27:50ZDereplication of antimicrobial biosurfactants from marine bacteria using molecular networking10.1038/s41598-021-95788-92045-2322https://doaj.org/article/59d6c9e4ca76453689eebb08191524182021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95788-9https://doaj.org/toc/2045-2322Abstract Biosurfactants are amphiphilic surface-active molecules of microbial origin principally produced by hydrocarbon-degrading bacteria; in addition to the bioremediation properties, they can also present antimicrobial activity. The present study highlights the chemical characterization and the antimicrobial activities of biosurfactants produced by deep-sea marine bacteria from the genera Halomonas, Bacillus, Streptomyces, and Pseudomonas. The biosurfactants were extracted and chemically characterized through Chromatography TLC, FT-IR, LC/ESI–MS/MS, and a metabolic analysis was done through molecular networking. Six biosurfactants were identified by dereplication tools from GNPS and some surfactin isoforms were identified by molecular networking. The half-maximal inhibitory concentration (IC50) of biosurfactant from Halomonas sp. INV PRT125 (7.27 mg L−1) and Halomonas sp. INV PRT124 (8.92 mg L−1) were most effective against the pathogenic yeast Candida albicans ATCC 10231. For Methicillin-resistant Staphylococcus aureus ATCC 43300, the biosurfactant from Bacillus sp. INV FIR48 was the most effective with IC50 values of 25.65 mg L−1 and 21.54 mg L−1 for C. albicans, without hemolytic effect (< 1%), and non-ecotoxic effect in brine shrimp larvae (Artemia franciscana), with values under 150 mg L−1, being a biosurfactant promising for further study. The extreme environments as deep-sea can be an important source for the isolation of new biosurfactants-producing microorganisms with environmental and pharmaceutical use.Albert D. PatiñoManuela Montoya-GiraldoMarynes QuinteroLizbeth L. López-ParraLina M. BlandónJavier Gómez-LeónNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Albert D. Patiño Manuela Montoya-Giraldo Marynes Quintero Lizbeth L. López-Parra Lina M. Blandón Javier Gómez-León Dereplication of antimicrobial biosurfactants from marine bacteria using molecular networking
description	Abstract Biosurfactants are amphiphilic surface-active molecules of microbial origin principally produced by hydrocarbon-degrading bacteria; in addition to the bioremediation properties, they can also present antimicrobial activity. The present study highlights the chemical characterization and the antimicrobial activities of biosurfactants produced by deep-sea marine bacteria from the genera Halomonas, Bacillus, Streptomyces, and Pseudomonas. The biosurfactants were extracted and chemically characterized through Chromatography TLC, FT-IR, LC/ESI–MS/MS, and a metabolic analysis was done through molecular networking. Six biosurfactants were identified by dereplication tools from GNPS and some surfactin isoforms were identified by molecular networking. The half-maximal inhibitory concentration (IC50) of biosurfactant from Halomonas sp. INV PRT125 (7.27 mg L−1) and Halomonas sp. INV PRT124 (8.92 mg L−1) were most effective against the pathogenic yeast Candida albicans ATCC 10231. For Methicillin-resistant Staphylococcus aureus ATCC 43300, the biosurfactant from Bacillus sp. INV FIR48 was the most effective with IC50 values of 25.65 mg L−1 and 21.54 mg L−1 for C. albicans, without hemolytic effect (< 1%), and non-ecotoxic effect in brine shrimp larvae (Artemia franciscana), with values under 150 mg L−1, being a biosurfactant promising for further study. The extreme environments as deep-sea can be an important source for the isolation of new biosurfactants-producing microorganisms with environmental and pharmaceutical use.
format	article
author	Albert D. Patiño Manuela Montoya-Giraldo Marynes Quintero Lizbeth L. López-Parra Lina M. Blandón Javier Gómez-León
author_facet	Albert D. Patiño Manuela Montoya-Giraldo Marynes Quintero Lizbeth L. López-Parra Lina M. Blandón Javier Gómez-León
author_sort	Albert D. Patiño
title	Dereplication of antimicrobial biosurfactants from marine bacteria using molecular networking
title_short	Dereplication of antimicrobial biosurfactants from marine bacteria using molecular networking
title_full	Dereplication of antimicrobial biosurfactants from marine bacteria using molecular networking
title_fullStr	Dereplication of antimicrobial biosurfactants from marine bacteria using molecular networking
title_full_unstemmed	Dereplication of antimicrobial biosurfactants from marine bacteria using molecular networking
title_sort	dereplication of antimicrobial biosurfactants from marine bacteria using molecular networking
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/59d6c9e4ca76453689eebb0819152418
work_keys_str_mv	AT albertdpatino dereplicationofantimicrobialbiosurfactantsfrommarinebacteriausingmolecularnetworking AT manuelamontoyagiraldo dereplicationofantimicrobialbiosurfactantsfrommarinebacteriausingmolecularnetworking AT marynesquintero dereplicationofantimicrobialbiosurfactantsfrommarinebacteriausingmolecularnetworking AT lizbethllopezparra dereplicationofantimicrobialbiosurfactantsfrommarinebacteriausingmolecularnetworking AT linamblandon dereplicationofantimicrobialbiosurfactantsfrommarinebacteriausingmolecularnetworking AT javiergomezleon dereplicationofantimicrobialbiosurfactantsfrommarinebacteriausingmolecularnetworking
_version_	1718383992227495936

Dereplication of antimicrobial biosurfactants from marine bacteria using molecular networking

Ejemplares similares