Towards a Cardoon (Cynara cardunculus var. altilis)-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway

Cultivated cardoon (Cynara cardunculus var. altilis L.) is a promising candidate species for the development of plant cell cultures suitable for large-scale biomass production and recovery of nutraceuticals. We set up a protocol for Agrobacterium tumefa...

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Autores principales:	Dario Paolo, Franca Locatelli, Eleonora Cominelli, Raul Pirona, Sara Pozzo, Giulia Graziani, Alberto Ritieni, Monica De Palma, Teresa Docimo, Marina Tucci, Francesca Sparvoli
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	plant cell cultures lignin cellulose accessibility nutraceuticals MYB4 Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/59d7c252162c4530ac66f9d89621a918
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spelling	oai:doaj.org-article:59d7c252162c4530ac66f9d89621a9182021-11-11T17:23:52ZTowards a Cardoon (<i>Cynara cardunculus</i> var. <i>altilis</i>)-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway10.3390/ijms2221119781422-00671661-6596https://doaj.org/article/59d7c252162c4530ac66f9d89621a9182021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11978https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Cultivated cardoon (<i>Cynara cardunculus</i> var. <i>altilis</i> L.) is a promising candidate species for the development of plant cell cultures suitable for large-scale biomass production and recovery of nutraceuticals. We set up a protocol for <i>Agrobacterium tumefaciens</i>-mediated transformation, which can be used for the improvement of cardoon cell cultures in a frame of biorefinery. As high lignin content determines lower saccharification yields for the biomass, we opted for a biotechnological approach, with the purpose of reducing lignin content; we generated transgenic lines overexpressing the <i>Arabidopsis thaliana</i> MYB4 transcription factor, a known repressor of lignin/flavonoid biosynthesis. Here, we report a comprehensive characterization, including metabolic and transcriptomic analyses of <i>AtMYB4</i> overexpression cardoon lines, in comparison to wild type, underlining favorable traits for their use in biorefinery. Among these, the improved accessibility of the lignocellulosic biomass to degrading enzymes due to depletion of lignin content, the unexpected increased growth rates, and the valuable nutraceutical profiles, in particular for hydroxycinnamic/caffeoylquinic and fatty acids profiles.Dario PaoloFranca LocatelliEleonora CominelliRaul PironaSara PozzoGiulia GrazianiAlberto RitieniMonica De PalmaTeresa DocimoMarina TucciFrancesca SparvoliMDPI AGarticleplant cell cultureslignincellulose accessibilitynutraceuticalsMYB4Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11978, p 11978 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	plant cell cultures lignin cellulose accessibility nutraceuticals MYB4 Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	plant cell cultures lignin cellulose accessibility nutraceuticals MYB4 Biology (General) QH301-705.5 Chemistry QD1-999 Dario Paolo Franca Locatelli Eleonora Cominelli Raul Pirona Sara Pozzo Giulia Graziani Alberto Ritieni Monica De Palma Teresa Docimo Marina Tucci Francesca Sparvoli Towards a Cardoon (<i>Cynara cardunculus</i> var. <i>altilis</i>)-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway
description	Cultivated cardoon (<i>Cynara cardunculus</i> var. <i>altilis</i> L.) is a promising candidate species for the development of plant cell cultures suitable for large-scale biomass production and recovery of nutraceuticals. We set up a protocol for <i>Agrobacterium tumefaciens</i>-mediated transformation, which can be used for the improvement of cardoon cell cultures in a frame of biorefinery. As high lignin content determines lower saccharification yields for the biomass, we opted for a biotechnological approach, with the purpose of reducing lignin content; we generated transgenic lines overexpressing the <i>Arabidopsis thaliana</i> MYB4 transcription factor, a known repressor of lignin/flavonoid biosynthesis. Here, we report a comprehensive characterization, including metabolic and transcriptomic analyses of <i>AtMYB4</i> overexpression cardoon lines, in comparison to wild type, underlining favorable traits for their use in biorefinery. Among these, the improved accessibility of the lignocellulosic biomass to degrading enzymes due to depletion of lignin content, the unexpected increased growth rates, and the valuable nutraceutical profiles, in particular for hydroxycinnamic/caffeoylquinic and fatty acids profiles.
format	article
author	Dario Paolo Franca Locatelli Eleonora Cominelli Raul Pirona Sara Pozzo Giulia Graziani Alberto Ritieni Monica De Palma Teresa Docimo Marina Tucci Francesca Sparvoli
author_facet	Dario Paolo Franca Locatelli Eleonora Cominelli Raul Pirona Sara Pozzo Giulia Graziani Alberto Ritieni Monica De Palma Teresa Docimo Marina Tucci Francesca Sparvoli
author_sort	Dario Paolo
title	Towards a Cardoon (<i>Cynara cardunculus</i> var. <i>altilis</i>)-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway
title_short	Towards a Cardoon (<i>Cynara cardunculus</i> var. <i>altilis</i>)-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway
title_full	Towards a Cardoon (<i>Cynara cardunculus</i> var. <i>altilis</i>)-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway
title_fullStr	Towards a Cardoon (<i>Cynara cardunculus</i> var. <i>altilis</i>)-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway
title_full_unstemmed	Towards a Cardoon (<i>Cynara cardunculus</i> var. <i>altilis</i>)-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway
title_sort	towards a cardoon (<i>cynara cardunculus</i> var. <i>altilis</i>)-based biorefinery: a case study of improved cell cultures via genetic modulation of the phenylpropanoid pathway
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/59d7c252162c4530ac66f9d89621a918
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Towards a Cardoon (<i>Cynara cardunculus</i> var. <i>altilis</i>)-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway

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