Diverse Krill Lipid Fractions Differentially Reduce LPS-Induced Inflammatory Markers in RAW264.7 Macrophages In Vitro

Antarctic krill oil is an emerging marine lipid and expected to be a potential functional food due to its diverse nutrients, such as eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), phospholipids, astaxanthin and tocopherols. Although krill oil has been previously proved to have anti-inflamm...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dan Xie, Fangyuan He, Xiaosan Wang, Xingguo Wang, Qingzhe Jin, Jun Jin
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	krill oil anti-inflammatory effect RAW 264.7 cell chemical composition Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/59ddd7f2cc2f47e6861cc9366fcf9892
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:59ddd7f2cc2f47e6861cc9366fcf9892
record_format	dspace
spelling	oai:doaj.org-article:59ddd7f2cc2f47e6861cc9366fcf98922021-11-25T17:36:57ZDiverse Krill Lipid Fractions Differentially Reduce LPS-Induced Inflammatory Markers in RAW264.7 Macrophages In Vitro10.3390/foods101128872304-8158https://doaj.org/article/59ddd7f2cc2f47e6861cc9366fcf98922021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2887https://doaj.org/toc/2304-8158Antarctic krill oil is an emerging marine lipid and expected to be a potential functional food due to its diverse nutrients, such as eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), phospholipids, astaxanthin and tocopherols. Although krill oil has been previously proved to have anti-inflammatory activity, there is little information about the relationship between its chemical compositions and anti-inflammatory activity. In this study, the RAW264.7 macrophages model was used to elucidate and compare the anti-inflammatory potential of different krill lipid fractions: KLF-A, KLF-H and KLF-E, which have increasing phospholipids, EPA and DHA contents but decreasing astaxanthin and tocopherols levels. Results showed that all the krill lipid fractions alleviated the inflammatory reaction by inhibition of production of nitric oxide (NO), release of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 and gene expression of proinflammatory mediators including TNF-α, IL-1β, IL-6, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). In addition, KLF-E with the highest phospholipids, EPA and DHA contents showed the strongest inhibition effect on the LPS-induced proinflammatory mediator release and their gene expressions. The results would be helpful to provide powerful insights into the underlying anti-inflammatory mechanism of krill lipid and guiding the production of krill oil products with tailor-made anti-inflammatory activity.Dan XieFangyuan HeXiaosan WangXingguo WangQingzhe JinJun JinMDPI AGarticlekrill oilanti-inflammatory effectRAW 264.7 cellchemical compositionChemical technologyTP1-1185ENFoods, Vol 10, Iss 2887, p 2887 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	krill oil anti-inflammatory effect RAW 264.7 cell chemical composition Chemical technology TP1-1185
spellingShingle	krill oil anti-inflammatory effect RAW 264.7 cell chemical composition Chemical technology TP1-1185 Dan Xie Fangyuan He Xiaosan Wang Xingguo Wang Qingzhe Jin Jun Jin Diverse Krill Lipid Fractions Differentially Reduce LPS-Induced Inflammatory Markers in RAW264.7 Macrophages In Vitro
description	Antarctic krill oil is an emerging marine lipid and expected to be a potential functional food due to its diverse nutrients, such as eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), phospholipids, astaxanthin and tocopherols. Although krill oil has been previously proved to have anti-inflammatory activity, there is little information about the relationship between its chemical compositions and anti-inflammatory activity. In this study, the RAW264.7 macrophages model was used to elucidate and compare the anti-inflammatory potential of different krill lipid fractions: KLF-A, KLF-H and KLF-E, which have increasing phospholipids, EPA and DHA contents but decreasing astaxanthin and tocopherols levels. Results showed that all the krill lipid fractions alleviated the inflammatory reaction by inhibition of production of nitric oxide (NO), release of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 and gene expression of proinflammatory mediators including TNF-α, IL-1β, IL-6, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). In addition, KLF-E with the highest phospholipids, EPA and DHA contents showed the strongest inhibition effect on the LPS-induced proinflammatory mediator release and their gene expressions. The results would be helpful to provide powerful insights into the underlying anti-inflammatory mechanism of krill lipid and guiding the production of krill oil products with tailor-made anti-inflammatory activity.
format	article
author	Dan Xie Fangyuan He Xiaosan Wang Xingguo Wang Qingzhe Jin Jun Jin
author_facet	Dan Xie Fangyuan He Xiaosan Wang Xingguo Wang Qingzhe Jin Jun Jin
author_sort	Dan Xie
title	Diverse Krill Lipid Fractions Differentially Reduce LPS-Induced Inflammatory Markers in RAW264.7 Macrophages In Vitro
title_short	Diverse Krill Lipid Fractions Differentially Reduce LPS-Induced Inflammatory Markers in RAW264.7 Macrophages In Vitro
title_full	Diverse Krill Lipid Fractions Differentially Reduce LPS-Induced Inflammatory Markers in RAW264.7 Macrophages In Vitro
title_fullStr	Diverse Krill Lipid Fractions Differentially Reduce LPS-Induced Inflammatory Markers in RAW264.7 Macrophages In Vitro
title_full_unstemmed	Diverse Krill Lipid Fractions Differentially Reduce LPS-Induced Inflammatory Markers in RAW264.7 Macrophages In Vitro
title_sort	diverse krill lipid fractions differentially reduce lps-induced inflammatory markers in raw264.7 macrophages in vitro
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/59ddd7f2cc2f47e6861cc9366fcf9892
work_keys_str_mv	AT danxie diversekrilllipidfractionsdifferentiallyreducelpsinducedinflammatorymarkersinraw2647macrophagesinvitro AT fangyuanhe diversekrilllipidfractionsdifferentiallyreducelpsinducedinflammatorymarkersinraw2647macrophagesinvitro AT xiaosanwang diversekrilllipidfractionsdifferentiallyreducelpsinducedinflammatorymarkersinraw2647macrophagesinvitro AT xingguowang diversekrilllipidfractionsdifferentiallyreducelpsinducedinflammatorymarkersinraw2647macrophagesinvitro AT qingzhejin diversekrilllipidfractionsdifferentiallyreducelpsinducedinflammatorymarkersinraw2647macrophagesinvitro AT junjin diversekrilllipidfractionsdifferentiallyreducelpsinducedinflammatorymarkersinraw2647macrophagesinvitro
_version_	1718412165499584512

Diverse Krill Lipid Fractions Differentially Reduce LPS-Induced Inflammatory Markers in RAW264.7 Macrophages In Vitro

Ejemplares similares