Scolicidal Effects of Nanoparticles Against Hydatid Cyst Protoscolices in vitro

Roghayeh Norouzi,1 Amin Ataei,1 Marzie Hejazy,2 Ayman Noreddin,3,4 Mohamed Ezzat El Zowalaty5 1Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran; 2Department of Basic Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran; 3Infectious...

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Autores principales:	Norouzi R, Ataei A, Hejazy M, Noreddin A, El Zowalaty ME
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	scolicidal hydatid cyst protoscolices silver nanoparticle iron nanoparticle copper nanoparticle silica nanoparticle in vitro. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/59e526c86a424bf5bffe569e075ba65e
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spelling	oai:doaj.org-article:59e526c86a424bf5bffe569e075ba65e2021-12-02T06:42:14ZScolicidal Effects of Nanoparticles Against Hydatid Cyst Protoscolices in vitro1178-2013https://doaj.org/article/59e526c86a424bf5bffe569e075ba65e2020-02-01T00:00:00Zhttps://www.dovepress.com/scolicidal-effects-of-nanoparticles-against-hydatid-cyst-protoscolices-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Roghayeh Norouzi,1 Amin Ataei,1 Marzie Hejazy,2 Ayman Noreddin,3,4 Mohamed Ezzat El Zowalaty5 1Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran; 2Department of Basic Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran; 3Infectious Diseases and Anti-Infective Therapy Research Group, Sharjah Medical Research Institute and College of Pharmacy, University of Sharjah, Sharjah 27272, United Arab Emirates; 4Department of Medicine, School of Medicine, University of California Irvine, Irvine, CA, USA; 5Department of Pharmacy, City University College of Ajman, Ajman 18484, United Arab EmiratesCorrespondence: Roghayeh NorouziDepartment of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, IranEmail roghayehnorouzi123@gmail.comMohamed Ezzat El ZowalatyDepartment of Pharmacy, City University College of Ajman, Ajman 18484, United Arab EmiratesEmail elzow005@gmail.comBackground: Echinococcus granulosus is causative agent of cystic echinococcosis (CE), which has a cosmopolitan distribution. The current methods for the treatment of human CE include surgery. Therefore, the development of new scolicidal agents with low side effects and more efficacies is an urgent need.Purpose: The present study aimed to compare the scolicidal efficacies of silver, iron, copper, silica and zinc oxide nanoparticles (NPs) against hydatid cyst protoscolices in vitro.Methods: Hydatid cysts of sheep liver and lung were collected. The cyst fluid containing protoscolices was aspirated aseptically. The scolicidal activities of the silver, iron, copper, silica and zinc nanoparticles (Ag-NP, Fe-NP, Cu-NP, Si-NP and Zn-NP) were tested at different concentrations of 0.25, 0.5 and 1 mg/mL following 10, 30 and 60 min of incubation in triplicate. Viability of protoscolices was confirmed by 0.1% eosin staining.Results: Results showed that Ag-NPs at all concentrations tested had the highest scolicidal effect. Ag-NPs at 1 mg/mL concentration after 60 min of exposure time showed 80% mortality rate. Si-NPs had the high scolicidal activity at 1 mg/mL concentration (52.33%), Cu-NPs at 0.5 mg/mL concentration (41%), Fe-NPs at 1mg/mL concentration (28%) and Zn-NPs at concentration of 1mg/mL after 60 mins (15.67%).Conclusion: The findings of the present study showed that Ag-NPs, Fe-NPs, Cu-NPs, Si-NPs and Zn-NPs had potent scolicidal effects and that Ag-NPs are recommended as effective scolicidal agents. However, further in vivo studies are required to evaluate the efficacy of these nanoparticles.Keywords: scolicidal, hydatid cyst, protoscolices, silver nanoparticle, iron nanoparticle, copper nanoparticle, silica nanoparticle, in vitroNorouzi RAtaei AHejazy MNoreddin AEl Zowalaty MEDove Medical Pressarticlescolicidalhydatid cystprotoscolicessilver nanoparticleiron nanoparticlecopper nanoparticlesilica nanoparticlein vitro.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 1095-1100 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	scolicidal hydatid cyst protoscolices silver nanoparticle iron nanoparticle copper nanoparticle silica nanoparticle in vitro. Medicine (General) R5-920
spellingShingle	scolicidal hydatid cyst protoscolices silver nanoparticle iron nanoparticle copper nanoparticle silica nanoparticle in vitro. Medicine (General) R5-920 Norouzi R Ataei A Hejazy M Noreddin A El Zowalaty ME Scolicidal Effects of Nanoparticles Against Hydatid Cyst Protoscolices in vitro
description	Roghayeh Norouzi,1 Amin Ataei,1 Marzie Hejazy,2 Ayman Noreddin,3,4 Mohamed Ezzat El Zowalaty5 1Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran; 2Department of Basic Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran; 3Infectious Diseases and Anti-Infective Therapy Research Group, Sharjah Medical Research Institute and College of Pharmacy, University of Sharjah, Sharjah 27272, United Arab Emirates; 4Department of Medicine, School of Medicine, University of California Irvine, Irvine, CA, USA; 5Department of Pharmacy, City University College of Ajman, Ajman 18484, United Arab EmiratesCorrespondence: Roghayeh NorouziDepartment of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, IranEmail roghayehnorouzi123@gmail.comMohamed Ezzat El ZowalatyDepartment of Pharmacy, City University College of Ajman, Ajman 18484, United Arab EmiratesEmail elzow005@gmail.comBackground: Echinococcus granulosus is causative agent of cystic echinococcosis (CE), which has a cosmopolitan distribution. The current methods for the treatment of human CE include surgery. Therefore, the development of new scolicidal agents with low side effects and more efficacies is an urgent need.Purpose: The present study aimed to compare the scolicidal efficacies of silver, iron, copper, silica and zinc oxide nanoparticles (NPs) against hydatid cyst protoscolices in vitro.Methods: Hydatid cysts of sheep liver and lung were collected. The cyst fluid containing protoscolices was aspirated aseptically. The scolicidal activities of the silver, iron, copper, silica and zinc nanoparticles (Ag-NP, Fe-NP, Cu-NP, Si-NP and Zn-NP) were tested at different concentrations of 0.25, 0.5 and 1 mg/mL following 10, 30 and 60 min of incubation in triplicate. Viability of protoscolices was confirmed by 0.1% eosin staining.Results: Results showed that Ag-NPs at all concentrations tested had the highest scolicidal effect. Ag-NPs at 1 mg/mL concentration after 60 min of exposure time showed 80% mortality rate. Si-NPs had the high scolicidal activity at 1 mg/mL concentration (52.33%), Cu-NPs at 0.5 mg/mL concentration (41%), Fe-NPs at 1mg/mL concentration (28%) and Zn-NPs at concentration of 1mg/mL after 60 mins (15.67%).Conclusion: The findings of the present study showed that Ag-NPs, Fe-NPs, Cu-NPs, Si-NPs and Zn-NPs had potent scolicidal effects and that Ag-NPs are recommended as effective scolicidal agents. However, further in vivo studies are required to evaluate the efficacy of these nanoparticles.Keywords: scolicidal, hydatid cyst, protoscolices, silver nanoparticle, iron nanoparticle, copper nanoparticle, silica nanoparticle, in vitro
format	article
author	Norouzi R Ataei A Hejazy M Noreddin A El Zowalaty ME
author_facet	Norouzi R Ataei A Hejazy M Noreddin A El Zowalaty ME
author_sort	Norouzi R
title	Scolicidal Effects of Nanoparticles Against Hydatid Cyst Protoscolices in vitro
title_short	Scolicidal Effects of Nanoparticles Against Hydatid Cyst Protoscolices in vitro
title_full	Scolicidal Effects of Nanoparticles Against Hydatid Cyst Protoscolices in vitro
title_fullStr	Scolicidal Effects of Nanoparticles Against Hydatid Cyst Protoscolices in vitro
title_full_unstemmed	Scolicidal Effects of Nanoparticles Against Hydatid Cyst Protoscolices in vitro
title_sort	scolicidal effects of nanoparticles against hydatid cyst protoscolices in vitro
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/59e526c86a424bf5bffe569e075ba65e
work_keys_str_mv	AT norouzir scolicidaleffectsofnanoparticlesagainsthydatidcystprotoscolicesinvitro AT ataeia scolicidaleffectsofnanoparticlesagainsthydatidcystprotoscolicesinvitro AT hejazym scolicidaleffectsofnanoparticlesagainsthydatidcystprotoscolicesinvitro AT noreddina scolicidaleffectsofnanoparticlesagainsthydatidcystprotoscolicesinvitro AT elzowalatyme scolicidaleffectsofnanoparticlesagainsthydatidcystprotoscolicesinvitro
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Scolicidal Effects of Nanoparticles Against Hydatid Cyst Protoscolices in vitro

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