Species-Level Resolution of Female Bladder Microbiota from 16S rRNA Amplicon Sequencing

Accurate species-level identification from culture-independent techniques is of importance for microbial niches that are less well characterized, such as that of the bladder. 16S rRNA amplicon sequencing, a common culture-independent way to identify bacteria, is often critiqued for lacking species-...

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Autores principales:	Carter Hoffman, Nazema Y. Siddiqui, Ian Fields, W. Thomas Gregory, Holly M. Simon, Michael A. Mooney, Alan J. Wolfe, Lisa Karstens
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/59e6c0bfd33340598b2de6acd25dd310
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id	oai:doaj.org-article:59e6c0bfd33340598b2de6acd25dd310
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spelling	oai:doaj.org-article:59e6c0bfd33340598b2de6acd25dd3102021-12-02T16:51:26ZSpecies-Level Resolution of Female Bladder Microbiota from 16S rRNA Amplicon Sequencing2379-507710.1128/mSystems.00518-21https://doaj.org/article/59e6c0bfd33340598b2de6acd25dd3102021-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00518-21https://doaj.org/toc/2379-5077 Accurate species-level identification from culture-independent techniques is of importance for microbial niches that are less well characterized, such as that of the bladder. 16S rRNA amplicon sequencing, a common culture-independent way to identify bacteria, is often critiqued for lacking species-level resolution. Here, we extensively evaluate classification schemes for species-level bacterial annotation of 16S amplicon data from bladder bacteria.Carter HoffmanNazema Y. SiddiquiIan FieldsW. Thomas GregoryHolly M. SimonMichael A. MooneyAlan J. WolfeLisa KarstensAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmSystems, Vol 6, Iss 5 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Carter Hoffman Nazema Y. Siddiqui Ian Fields W. Thomas Gregory Holly M. Simon Michael A. Mooney Alan J. Wolfe Lisa Karstens Species-Level Resolution of Female Bladder Microbiota from 16S rRNA Amplicon Sequencing
description	Accurate species-level identification from culture-independent techniques is of importance for microbial niches that are less well characterized, such as that of the bladder. 16S rRNA amplicon sequencing, a common culture-independent way to identify bacteria, is often critiqued for lacking species-level resolution. Here, we extensively evaluate classification schemes for species-level bacterial annotation of 16S amplicon data from bladder bacteria.
format	article
author	Carter Hoffman Nazema Y. Siddiqui Ian Fields W. Thomas Gregory Holly M. Simon Michael A. Mooney Alan J. Wolfe Lisa Karstens
author_facet	Carter Hoffman Nazema Y. Siddiqui Ian Fields W. Thomas Gregory Holly M. Simon Michael A. Mooney Alan J. Wolfe Lisa Karstens
author_sort	Carter Hoffman
title	Species-Level Resolution of Female Bladder Microbiota from 16S rRNA Amplicon Sequencing
title_short	Species-Level Resolution of Female Bladder Microbiota from 16S rRNA Amplicon Sequencing
title_full	Species-Level Resolution of Female Bladder Microbiota from 16S rRNA Amplicon Sequencing
title_fullStr	Species-Level Resolution of Female Bladder Microbiota from 16S rRNA Amplicon Sequencing
title_full_unstemmed	Species-Level Resolution of Female Bladder Microbiota from 16S rRNA Amplicon Sequencing
title_sort	species-level resolution of female bladder microbiota from 16s rrna amplicon sequencing
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/59e6c0bfd33340598b2de6acd25dd310
work_keys_str_mv	AT carterhoffman specieslevelresolutionoffemalebladdermicrobiotafrom16srrnaampliconsequencing AT nazemaysiddiqui specieslevelresolutionoffemalebladdermicrobiotafrom16srrnaampliconsequencing AT ianfields specieslevelresolutionoffemalebladdermicrobiotafrom16srrnaampliconsequencing AT wthomasgregory specieslevelresolutionoffemalebladdermicrobiotafrom16srrnaampliconsequencing AT hollymsimon specieslevelresolutionoffemalebladdermicrobiotafrom16srrnaampliconsequencing AT michaelamooney specieslevelresolutionoffemalebladdermicrobiotafrom16srrnaampliconsequencing AT alanjwolfe specieslevelresolutionoffemalebladdermicrobiotafrom16srrnaampliconsequencing AT lisakarstens specieslevelresolutionoffemalebladdermicrobiotafrom16srrnaampliconsequencing
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Species-Level Resolution of Female Bladder Microbiota from 16S rRNA Amplicon Sequencing

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