High fecal carriage of bla CTX-M, bla CMY-2, and plasmid-mediated quinolone resistance genes among healthy Korean people in a metagenomic analysis

Abstract To characterize the carriage of antibiotic resistance genes (ARGs) in the gut microbiome of healthy individuals. Fecal carriage of ARGs was investigated in 61 healthy individuals aged 30 to 59 years through whole metagenome sequencing of the gut microbiome and a targeted metagenomic approac...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jieun Kim, Kye-Yeung Park, Hoon-Ki Park, Hwan-Sik Hwang, Mi-Ran Seo, Bongyoung Kim, Youna Cho, Mina Rho, Hyunjoo Pai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/59e8a6e489554840bc2eece9b2c91f93
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:59e8a6e489554840bc2eece9b2c91f93
record_format	dspace
spelling	oai:doaj.org-article:59e8a6e489554840bc2eece9b2c91f932021-12-02T15:52:21ZHigh fecal carriage of bla CTX-M, bla CMY-2, and plasmid-mediated quinolone resistance genes among healthy Korean people in a metagenomic analysis10.1038/s41598-021-84974-42045-2322https://doaj.org/article/59e8a6e489554840bc2eece9b2c91f932021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84974-4https://doaj.org/toc/2045-2322Abstract To characterize the carriage of antibiotic resistance genes (ARGs) in the gut microbiome of healthy individuals. Fecal carriage of ARGs was investigated in 61 healthy individuals aged 30 to 59 years through whole metagenome sequencing of the gut microbiome and a targeted metagenomic approach. The number of ARGs in the gut microbiome was counted and normalized per million predicted genes (GPM). In the Korean population, the resistome ranged from 49.7 to 292.5 GPM (median 89.7). Based on the abundance of ARGs, the subjects were categorised into high (> 120 GPM), middle (60‒120 GPM), and low (< 60 GPM) ARG groups. Individuals in the high ARG group tended to visit hospitals more often (P = 0.065), particularly for upper respiratory tract infections (P = 0.066), and carried more bla CTX-M (P = 0.008). The targeted metagenome approach for bla and plasmid-mediated quinolone resistance (PMQR) genes revealed a high fecal carriage rate; 23% or 13.1% of the subjects carried bla CTX-M or bla CMY-2, respectively. Regarding PMQR genes, 59% of the subjects carried PMQR, and 83% of them harboured 2‒4 PMQR genes (qnrB 44.3%, qnrS 47.5% etc.). The presence of bla CTX-M correlated with ARG abundance in the gut resistome, whereas PMQR genes were irrelevant to other ARGs (P = 0.176). Fecal carriage of bla CTX-M and PMQR genes was broad and multiplexed among healthy individuals.Jieun KimKye-Yeung ParkHoon-Ki ParkHwan-Sik HwangMi-Ran SeoBongyoung KimYouna ChoMina RhoHyunjoo PaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jieun Kim Kye-Yeung Park Hoon-Ki Park Hwan-Sik Hwang Mi-Ran Seo Bongyoung Kim Youna Cho Mina Rho Hyunjoo Pai High fecal carriage of bla CTX-M, bla CMY-2, and plasmid-mediated quinolone resistance genes among healthy Korean people in a metagenomic analysis
description	Abstract To characterize the carriage of antibiotic resistance genes (ARGs) in the gut microbiome of healthy individuals. Fecal carriage of ARGs was investigated in 61 healthy individuals aged 30 to 59 years through whole metagenome sequencing of the gut microbiome and a targeted metagenomic approach. The number of ARGs in the gut microbiome was counted and normalized per million predicted genes (GPM). In the Korean population, the resistome ranged from 49.7 to 292.5 GPM (median 89.7). Based on the abundance of ARGs, the subjects were categorised into high (> 120 GPM), middle (60‒120 GPM), and low (< 60 GPM) ARG groups. Individuals in the high ARG group tended to visit hospitals more often (P = 0.065), particularly for upper respiratory tract infections (P = 0.066), and carried more bla CTX-M (P = 0.008). The targeted metagenome approach for bla and plasmid-mediated quinolone resistance (PMQR) genes revealed a high fecal carriage rate; 23% or 13.1% of the subjects carried bla CTX-M or bla CMY-2, respectively. Regarding PMQR genes, 59% of the subjects carried PMQR, and 83% of them harboured 2‒4 PMQR genes (qnrB 44.3%, qnrS 47.5% etc.). The presence of bla CTX-M correlated with ARG abundance in the gut resistome, whereas PMQR genes were irrelevant to other ARGs (P = 0.176). Fecal carriage of bla CTX-M and PMQR genes was broad and multiplexed among healthy individuals.
format	article
author	Jieun Kim Kye-Yeung Park Hoon-Ki Park Hwan-Sik Hwang Mi-Ran Seo Bongyoung Kim Youna Cho Mina Rho Hyunjoo Pai
author_facet	Jieun Kim Kye-Yeung Park Hoon-Ki Park Hwan-Sik Hwang Mi-Ran Seo Bongyoung Kim Youna Cho Mina Rho Hyunjoo Pai
author_sort	Jieun Kim
title	High fecal carriage of bla CTX-M, bla CMY-2, and plasmid-mediated quinolone resistance genes among healthy Korean people in a metagenomic analysis
title_short	High fecal carriage of bla CTX-M, bla CMY-2, and plasmid-mediated quinolone resistance genes among healthy Korean people in a metagenomic analysis
title_full	High fecal carriage of bla CTX-M, bla CMY-2, and plasmid-mediated quinolone resistance genes among healthy Korean people in a metagenomic analysis
title_fullStr	High fecal carriage of bla CTX-M, bla CMY-2, and plasmid-mediated quinolone resistance genes among healthy Korean people in a metagenomic analysis
title_full_unstemmed	High fecal carriage of bla CTX-M, bla CMY-2, and plasmid-mediated quinolone resistance genes among healthy Korean people in a metagenomic analysis
title_sort	high fecal carriage of bla ctx-m, bla cmy-2, and plasmid-mediated quinolone resistance genes among healthy korean people in a metagenomic analysis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/59e8a6e489554840bc2eece9b2c91f93
work_keys_str_mv	AT jieunkim highfecalcarriageofblactxmblacmy2andplasmidmediatedquinoloneresistancegenesamonghealthykoreanpeopleinametagenomicanalysis AT kyeyeungpark highfecalcarriageofblactxmblacmy2andplasmidmediatedquinoloneresistancegenesamonghealthykoreanpeopleinametagenomicanalysis AT hoonkipark highfecalcarriageofblactxmblacmy2andplasmidmediatedquinoloneresistancegenesamonghealthykoreanpeopleinametagenomicanalysis AT hwansikhwang highfecalcarriageofblactxmblacmy2andplasmidmediatedquinoloneresistancegenesamonghealthykoreanpeopleinametagenomicanalysis AT miranseo highfecalcarriageofblactxmblacmy2andplasmidmediatedquinoloneresistancegenesamonghealthykoreanpeopleinametagenomicanalysis AT bongyoungkim highfecalcarriageofblactxmblacmy2andplasmidmediatedquinoloneresistancegenesamonghealthykoreanpeopleinametagenomicanalysis AT younacho highfecalcarriageofblactxmblacmy2andplasmidmediatedquinoloneresistancegenesamonghealthykoreanpeopleinametagenomicanalysis AT minarho highfecalcarriageofblactxmblacmy2andplasmidmediatedquinoloneresistancegenesamonghealthykoreanpeopleinametagenomicanalysis AT hyunjoopai highfecalcarriageofblactxmblacmy2andplasmidmediatedquinoloneresistancegenesamonghealthykoreanpeopleinametagenomicanalysis
_version_	1718385572322476032

High fecal carriage of bla CTX-M, bla CMY-2, and plasmid-mediated quinolone resistance genes among healthy Korean people in a metagenomic analysis

Ejemplares similares