Confocal imaging of single BaTiO3 nanoparticles by two-photon photothermal microscopy

Abstract We report on the development of a nonlinear optical microscopic technique based on two-photon absorption induced photothermal effect capable of detecting individual nonfluorescent nanoparticles with high sensitivity. The method which is inherently confocal makes use of near infrared excitat...

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Autores principales:	M. M. Bijeesh, P. K. Shakhi, S. Arunkarthick, Geetha K. Varier, P. Nandakumar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/59ec3cdacf40407eaa5b0042a4bc45e5
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spelling	oai:doaj.org-article:59ec3cdacf40407eaa5b0042a4bc45e52021-12-02T16:06:01ZConfocal imaging of single BaTiO3 nanoparticles by two-photon photothermal microscopy10.1038/s41598-017-01548-z2045-2322https://doaj.org/article/59ec3cdacf40407eaa5b0042a4bc45e52017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01548-zhttps://doaj.org/toc/2045-2322Abstract We report on the development of a nonlinear optical microscopic technique based on two-photon absorption induced photothermal effect capable of detecting individual nonfluorescent nanoparticles with high sensitivity. The method which is inherently confocal makes use of near infrared excitation at high repetition rates and would be of interest in deep tissue imaging. We demonstrate the applicability of the technique by imaging single BaTiO3 nanoparticles, a potential biomolecular label having high photostability, in a scattering environment at fast time scales with a pixel dwell time of 80 μs.M. M. BijeeshP. K. ShakhiS. ArunkarthickGeetha K. VarierP. NandakumarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M. M. Bijeesh P. K. Shakhi S. Arunkarthick Geetha K. Varier P. Nandakumar Confocal imaging of single BaTiO3 nanoparticles by two-photon photothermal microscopy
description	Abstract We report on the development of a nonlinear optical microscopic technique based on two-photon absorption induced photothermal effect capable of detecting individual nonfluorescent nanoparticles with high sensitivity. The method which is inherently confocal makes use of near infrared excitation at high repetition rates and would be of interest in deep tissue imaging. We demonstrate the applicability of the technique by imaging single BaTiO3 nanoparticles, a potential biomolecular label having high photostability, in a scattering environment at fast time scales with a pixel dwell time of 80 μs.
format	article
author	M. M. Bijeesh P. K. Shakhi S. Arunkarthick Geetha K. Varier P. Nandakumar
author_facet	M. M. Bijeesh P. K. Shakhi S. Arunkarthick Geetha K. Varier P. Nandakumar
author_sort	M. M. Bijeesh
title	Confocal imaging of single BaTiO3 nanoparticles by two-photon photothermal microscopy
title_short	Confocal imaging of single BaTiO3 nanoparticles by two-photon photothermal microscopy
title_full	Confocal imaging of single BaTiO3 nanoparticles by two-photon photothermal microscopy
title_fullStr	Confocal imaging of single BaTiO3 nanoparticles by two-photon photothermal microscopy
title_full_unstemmed	Confocal imaging of single BaTiO3 nanoparticles by two-photon photothermal microscopy
title_sort	confocal imaging of single batio3 nanoparticles by two-photon photothermal microscopy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/59ec3cdacf40407eaa5b0042a4bc45e5
work_keys_str_mv	AT mmbijeesh confocalimagingofsinglebatio3nanoparticlesbytwophotonphotothermalmicroscopy AT pkshakhi confocalimagingofsinglebatio3nanoparticlesbytwophotonphotothermalmicroscopy AT sarunkarthick confocalimagingofsinglebatio3nanoparticlesbytwophotonphotothermalmicroscopy AT geethakvarier confocalimagingofsinglebatio3nanoparticlesbytwophotonphotothermalmicroscopy AT pnandakumar confocalimagingofsinglebatio3nanoparticlesbytwophotonphotothermalmicroscopy
_version_	1718385150040997888

Confocal imaging of single BaTiO3 nanoparticles by two-photon photothermal microscopy

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