Guava Leaf Extract Attenuates Insulin Resistance via the PI3K/Akt Signaling Pathway in a Type 2 Diabetic Mouse Model

Qiong Yang,1,* Yu-Mei Wen,1,* Jing Shen,1 Mei-Mei Chen,1 Jiang-Hua Wen,2 Zheng-Ming Li,3 Yu-Zhen Liang,3,* Ning Xia1,* 1Department of Endocrinology and Metabolism, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, People’s Republic of China; 2Department of Health...

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Autores principales: Yang Q, Wen YM, Shen J, Chen MM, Wen JH, Li ZM, Liang YZ, Xia N
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Publicado: Dove Medical Press 2020
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spelling oai:doaj.org-article:59edf124513c4aecb23ccc2f706a08fe2021-12-02T13:20:15ZGuava Leaf Extract Attenuates Insulin Resistance via the PI3K/Akt Signaling Pathway in a Type 2 Diabetic Mouse Model1178-7007https://doaj.org/article/59edf124513c4aecb23ccc2f706a08fe2020-03-01T00:00:00Zhttps://www.dovepress.com/guava-leaf-extract-attenuates-insulin-resistance-via-the-pi3kakt-signa-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Qiong Yang,1,* Yu-Mei Wen,1,* Jing Shen,1 Mei-Mei Chen,1 Jiang-Hua Wen,2 Zheng-Ming Li,3 Yu-Zhen Liang,3,* Ning Xia1,* 1Department of Endocrinology and Metabolism, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, People’s Republic of China; 2Department of Health Examination, Jiangmen Central Hospital, Jiangmen, Guangdong 529070, People’s Republic of China; 3Department of Endocrinology and Metabolism, The Second Affiliated Hospital of Guangxi Medical University, Nanning 530021, People’s Republic of China*These authors contributed equally to this workCorrespondence: Ning XiaDepartment of Endocrinology and Metabolism, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, People’s Republic of ChinaTel +86 77 1280 2380Email xianinggxmu@163.comBackground and Objective: Insulin resistance is well known to exhibit essential effects on the progression of diabetes mellitus (DM). Guava leaf was also reported to exhibit anti-diabetic effects including decreasing blood glucose. Therefore, this present study aims to explore the role guava leaf extract (GLE) plays in insulin resistance and its mechanism of action via the PI3K/Akt signaling pathway.Methods: KK-Ay mice is a spontaneous genetic type 2 diabetes mouse model induced by feeding a high fat and high sugar diet. Mice were randomly assigned into three groups: diabetic mice (DM), DM + MET (diabetic mice treated with metformin) and DM + GLE (diabetic mice treated with GLE) groups. After 8 weeks of treatment, body weight and levels of fasting plasma glucose (FPG), fasting insulin and lipids in plasma were measured. Mice were sacrificed and mRNA and protein expression of insulin receptor substrate1 (IRS1), phosphatidylinositol 3-kinase (PI3K) and serine/threonine kinase protein B (Akt) in livers were measured.Results: GLE markedly reduced body weight, FPG, fasting insulin and insulin resistance index but increased the insulin sensitivity index of diabetic KK-Ay mice. Moreover, GLE upregulated the expression of IRS-1, PI3K and Akt mRNAs in livers of diabetic KK-Ay mice. In addition, GLE also elevated IRS-1, PI3K, Akt, p-PI3K and p-Akt protein expression in their livers. The results of the DM + MET group were similar to those of the DM + GLE group.Conclusion: GLE plays anti-diabetic roles by ameliorating insulin resistance in KK-Ay diabetic mice and this is related to the activation of PI3K/Akt signaling pathway.Keywords: diabetes, guava leaf extract, insulin resistance, PI3K/Akt signaling pathwayYang QWen YMShen JChen MMWen JHLi ZMLiang YZXia NDove Medical Pressarticlediabetesguava leaf extractinsulin resistancepi3k/akt signaling pathwaySpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 713-718 (2020)
institution DOAJ
collection DOAJ
language EN
topic diabetes
guava leaf extract
insulin resistance
pi3k/akt signaling pathway
Specialties of internal medicine
RC581-951
spellingShingle diabetes
guava leaf extract
insulin resistance
pi3k/akt signaling pathway
Specialties of internal medicine
RC581-951
Yang Q
Wen YM
Shen J
Chen MM
Wen JH
Li ZM
Liang YZ
Xia N
Guava Leaf Extract Attenuates Insulin Resistance via the PI3K/Akt Signaling Pathway in a Type 2 Diabetic Mouse Model
description Qiong Yang,1,* Yu-Mei Wen,1,* Jing Shen,1 Mei-Mei Chen,1 Jiang-Hua Wen,2 Zheng-Ming Li,3 Yu-Zhen Liang,3,* Ning Xia1,* 1Department of Endocrinology and Metabolism, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, People’s Republic of China; 2Department of Health Examination, Jiangmen Central Hospital, Jiangmen, Guangdong 529070, People’s Republic of China; 3Department of Endocrinology and Metabolism, The Second Affiliated Hospital of Guangxi Medical University, Nanning 530021, People’s Republic of China*These authors contributed equally to this workCorrespondence: Ning XiaDepartment of Endocrinology and Metabolism, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, People’s Republic of ChinaTel +86 77 1280 2380Email xianinggxmu@163.comBackground and Objective: Insulin resistance is well known to exhibit essential effects on the progression of diabetes mellitus (DM). Guava leaf was also reported to exhibit anti-diabetic effects including decreasing blood glucose. Therefore, this present study aims to explore the role guava leaf extract (GLE) plays in insulin resistance and its mechanism of action via the PI3K/Akt signaling pathway.Methods: KK-Ay mice is a spontaneous genetic type 2 diabetes mouse model induced by feeding a high fat and high sugar diet. Mice were randomly assigned into three groups: diabetic mice (DM), DM + MET (diabetic mice treated with metformin) and DM + GLE (diabetic mice treated with GLE) groups. After 8 weeks of treatment, body weight and levels of fasting plasma glucose (FPG), fasting insulin and lipids in plasma were measured. Mice were sacrificed and mRNA and protein expression of insulin receptor substrate1 (IRS1), phosphatidylinositol 3-kinase (PI3K) and serine/threonine kinase protein B (Akt) in livers were measured.Results: GLE markedly reduced body weight, FPG, fasting insulin and insulin resistance index but increased the insulin sensitivity index of diabetic KK-Ay mice. Moreover, GLE upregulated the expression of IRS-1, PI3K and Akt mRNAs in livers of diabetic KK-Ay mice. In addition, GLE also elevated IRS-1, PI3K, Akt, p-PI3K and p-Akt protein expression in their livers. The results of the DM + MET group were similar to those of the DM + GLE group.Conclusion: GLE plays anti-diabetic roles by ameliorating insulin resistance in KK-Ay diabetic mice and this is related to the activation of PI3K/Akt signaling pathway.Keywords: diabetes, guava leaf extract, insulin resistance, PI3K/Akt signaling pathway
format article
author Yang Q
Wen YM
Shen J
Chen MM
Wen JH
Li ZM
Liang YZ
Xia N
author_facet Yang Q
Wen YM
Shen J
Chen MM
Wen JH
Li ZM
Liang YZ
Xia N
author_sort Yang Q
title Guava Leaf Extract Attenuates Insulin Resistance via the PI3K/Akt Signaling Pathway in a Type 2 Diabetic Mouse Model
title_short Guava Leaf Extract Attenuates Insulin Resistance via the PI3K/Akt Signaling Pathway in a Type 2 Diabetic Mouse Model
title_full Guava Leaf Extract Attenuates Insulin Resistance via the PI3K/Akt Signaling Pathway in a Type 2 Diabetic Mouse Model
title_fullStr Guava Leaf Extract Attenuates Insulin Resistance via the PI3K/Akt Signaling Pathway in a Type 2 Diabetic Mouse Model
title_full_unstemmed Guava Leaf Extract Attenuates Insulin Resistance via the PI3K/Akt Signaling Pathway in a Type 2 Diabetic Mouse Model
title_sort guava leaf extract attenuates insulin resistance via the pi3k/akt signaling pathway in a type 2 diabetic mouse model
publisher Dove Medical Press
publishDate 2020
url https://doaj.org/article/59edf124513c4aecb23ccc2f706a08fe
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