Leukocytes with chromosome Y loss have reduced abundance of the cell surface immunoprotein CD99

Abstract Mosaic loss of chromosome Y (LOY) in immune cells is a male-specific mutation associated with increased risk for morbidity and mortality. The CD99 gene, positioned in the pseudoautosomal regions of chromosomes X and Y, encodes a cell surface protein essential for several key properties of l...

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Autores principales:	Jonas Mattisson, Marcus Danielsson, Maria Hammond, Hanna Davies, Caroline J. Gallant, Jessica Nordlund, Amanda Raine, Malin Edén, Lena Kilander, Martin Ingelsson, Jan P. Dumanski, Jonatan Halvardson, Lars A. Forsberg
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/59f0cb315b52492d8a02d3b3ffbc2609
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spelling	oai:doaj.org-article:59f0cb315b52492d8a02d3b3ffbc26092021-12-02T16:06:43ZLeukocytes with chromosome Y loss have reduced abundance of the cell surface immunoprotein CD9910.1038/s41598-021-94588-52045-2322https://doaj.org/article/59f0cb315b52492d8a02d3b3ffbc26092021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94588-5https://doaj.org/toc/2045-2322Abstract Mosaic loss of chromosome Y (LOY) in immune cells is a male-specific mutation associated with increased risk for morbidity and mortality. The CD99 gene, positioned in the pseudoautosomal regions of chromosomes X and Y, encodes a cell surface protein essential for several key properties of leukocytes and immune system functions. Here we used CITE-seq for simultaneous quantification of CD99 derived mRNA and cell surface CD99 protein abundance in relation to LOY in single cells. The abundance of CD99 molecules was lower on the surfaces of LOY cells compared with cells without this aneuploidy in all six types of leukocytes studied, while the abundance of CD proteins encoded by genes located on autosomal chromosomes were independent from LOY. These results connect LOY in single cells with immune related cellular properties at the protein level, providing mechanistic insight regarding disease vulnerability in men affected with mosaic chromosome Y loss in blood leukocytes.Jonas MattissonMarcus DanielssonMaria HammondHanna DaviesCaroline J. GallantJessica NordlundAmanda RaineMalin EdénLena KilanderMartin IngelssonJan P. DumanskiJonatan HalvardsonLars A. ForsbergNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jonas Mattisson Marcus Danielsson Maria Hammond Hanna Davies Caroline J. Gallant Jessica Nordlund Amanda Raine Malin Edén Lena Kilander Martin Ingelsson Jan P. Dumanski Jonatan Halvardson Lars A. Forsberg Leukocytes with chromosome Y loss have reduced abundance of the cell surface immunoprotein CD99
description	Abstract Mosaic loss of chromosome Y (LOY) in immune cells is a male-specific mutation associated with increased risk for morbidity and mortality. The CD99 gene, positioned in the pseudoautosomal regions of chromosomes X and Y, encodes a cell surface protein essential for several key properties of leukocytes and immune system functions. Here we used CITE-seq for simultaneous quantification of CD99 derived mRNA and cell surface CD99 protein abundance in relation to LOY in single cells. The abundance of CD99 molecules was lower on the surfaces of LOY cells compared with cells without this aneuploidy in all six types of leukocytes studied, while the abundance of CD proteins encoded by genes located on autosomal chromosomes were independent from LOY. These results connect LOY in single cells with immune related cellular properties at the protein level, providing mechanistic insight regarding disease vulnerability in men affected with mosaic chromosome Y loss in blood leukocytes.
format	article
author	Jonas Mattisson Marcus Danielsson Maria Hammond Hanna Davies Caroline J. Gallant Jessica Nordlund Amanda Raine Malin Edén Lena Kilander Martin Ingelsson Jan P. Dumanski Jonatan Halvardson Lars A. Forsberg
author_facet	Jonas Mattisson Marcus Danielsson Maria Hammond Hanna Davies Caroline J. Gallant Jessica Nordlund Amanda Raine Malin Edén Lena Kilander Martin Ingelsson Jan P. Dumanski Jonatan Halvardson Lars A. Forsberg
author_sort	Jonas Mattisson
title	Leukocytes with chromosome Y loss have reduced abundance of the cell surface immunoprotein CD99
title_short	Leukocytes with chromosome Y loss have reduced abundance of the cell surface immunoprotein CD99
title_full	Leukocytes with chromosome Y loss have reduced abundance of the cell surface immunoprotein CD99
title_fullStr	Leukocytes with chromosome Y loss have reduced abundance of the cell surface immunoprotein CD99
title_full_unstemmed	Leukocytes with chromosome Y loss have reduced abundance of the cell surface immunoprotein CD99
title_sort	leukocytes with chromosome y loss have reduced abundance of the cell surface immunoprotein cd99
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/59f0cb315b52492d8a02d3b3ffbc2609
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Leukocytes with chromosome Y loss have reduced abundance of the cell surface immunoprotein CD99

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