Active macromolecules of honey form colloidal particles essential for honey antibacterial activity and hydrogen peroxide production

Abstract Little is known about the global structure of honey and the arrangement of its main macromolecules. We hypothesized that the conditions in ripened honeys resemble macromolecular crowding in the cell and affect the concentration, reactivity, and conformation of honey macromolecules. Combined...

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Autores principales:	Katrina Brudzynski, Danielle Miotto, Linda Kim, Calvin Sjaarda, Liset Maldonado-Alvarez, Henryk Fukś
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/59f26e6d84e54068b4a7307f3e19d29a
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spelling	oai:doaj.org-article:59f26e6d84e54068b4a7307f3e19d29a2021-12-02T15:05:39ZActive macromolecules of honey form colloidal particles essential for honey antibacterial activity and hydrogen peroxide production10.1038/s41598-017-08072-02045-2322https://doaj.org/article/59f26e6d84e54068b4a7307f3e19d29a2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08072-0https://doaj.org/toc/2045-2322Abstract Little is known about the global structure of honey and the arrangement of its main macromolecules. We hypothesized that the conditions in ripened honeys resemble macromolecular crowding in the cell and affect the concentration, reactivity, and conformation of honey macromolecules. Combined results from UV spectroscopy, DLS and SEM showed that the concentration of macromolecules was a determining factor in honey structure. The UV spectral scans in 200–400 nm visualized and allowed quantification of UV-absorbing compounds in the following order: dark > medium > light honeys (p < 0.0001). The high concentration of macromolecules promoted their self-assembly to micron-size superstructures, visible in SEM as two-phase system consisting of dense globules distributed in sugar solution. These particles showed increased conformational stability upon dilution. At the threshold concentration, the system underwent phase transition with concomitant fragmentation of large micron-size particles to nanoparticles in hierarchical order. Honey two-phase conformation was an essential requirement for antibacterial activity and hydrogen peroxide production. These activities disappeared beyond the phase transition point. The realization that active macromolecules of honey are arranged into compact, stable multicomponent assemblies with colloidal properties reframes our view on global structure of honey and emerges as a key property to be considered in investigating its biological activity.Katrina BrudzynskiDanielle MiottoLinda KimCalvin SjaardaLiset Maldonado-AlvarezHenryk FukśNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katrina Brudzynski Danielle Miotto Linda Kim Calvin Sjaarda Liset Maldonado-Alvarez Henryk Fukś Active macromolecules of honey form colloidal particles essential for honey antibacterial activity and hydrogen peroxide production
description	Abstract Little is known about the global structure of honey and the arrangement of its main macromolecules. We hypothesized that the conditions in ripened honeys resemble macromolecular crowding in the cell and affect the concentration, reactivity, and conformation of honey macromolecules. Combined results from UV spectroscopy, DLS and SEM showed that the concentration of macromolecules was a determining factor in honey structure. The UV spectral scans in 200–400 nm visualized and allowed quantification of UV-absorbing compounds in the following order: dark > medium > light honeys (p < 0.0001). The high concentration of macromolecules promoted their self-assembly to micron-size superstructures, visible in SEM as two-phase system consisting of dense globules distributed in sugar solution. These particles showed increased conformational stability upon dilution. At the threshold concentration, the system underwent phase transition with concomitant fragmentation of large micron-size particles to nanoparticles in hierarchical order. Honey two-phase conformation was an essential requirement for antibacterial activity and hydrogen peroxide production. These activities disappeared beyond the phase transition point. The realization that active macromolecules of honey are arranged into compact, stable multicomponent assemblies with colloidal properties reframes our view on global structure of honey and emerges as a key property to be considered in investigating its biological activity.
format	article
author	Katrina Brudzynski Danielle Miotto Linda Kim Calvin Sjaarda Liset Maldonado-Alvarez Henryk Fukś
author_facet	Katrina Brudzynski Danielle Miotto Linda Kim Calvin Sjaarda Liset Maldonado-Alvarez Henryk Fukś
author_sort	Katrina Brudzynski
title	Active macromolecules of honey form colloidal particles essential for honey antibacterial activity and hydrogen peroxide production
title_short	Active macromolecules of honey form colloidal particles essential for honey antibacterial activity and hydrogen peroxide production
title_full	Active macromolecules of honey form colloidal particles essential for honey antibacterial activity and hydrogen peroxide production
title_fullStr	Active macromolecules of honey form colloidal particles essential for honey antibacterial activity and hydrogen peroxide production
title_full_unstemmed	Active macromolecules of honey form colloidal particles essential for honey antibacterial activity and hydrogen peroxide production
title_sort	active macromolecules of honey form colloidal particles essential for honey antibacterial activity and hydrogen peroxide production
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/59f26e6d84e54068b4a7307f3e19d29a
work_keys_str_mv	AT katrinabrudzynski activemacromoleculesofhoneyformcolloidalparticlesessentialforhoneyantibacterialactivityandhydrogenperoxideproduction AT daniellemiotto activemacromoleculesofhoneyformcolloidalparticlesessentialforhoneyantibacterialactivityandhydrogenperoxideproduction AT lindakim activemacromoleculesofhoneyformcolloidalparticlesessentialforhoneyantibacterialactivityandhydrogenperoxideproduction AT calvinsjaarda activemacromoleculesofhoneyformcolloidalparticlesessentialforhoneyantibacterialactivityandhydrogenperoxideproduction AT lisetmaldonadoalvarez activemacromoleculesofhoneyformcolloidalparticlesessentialforhoneyantibacterialactivityandhydrogenperoxideproduction AT henrykfuks activemacromoleculesofhoneyformcolloidalparticlesessentialforhoneyantibacterialactivityandhydrogenperoxideproduction
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Active macromolecules of honey form colloidal particles essential for honey antibacterial activity and hydrogen peroxide production

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