Quantum-dot-based immunochromatographic assay for total IgE in human serum.

To rapidly quantify total immunoglobulin E levels in human serum, we developed a novel quantum-dot-based immunochromatographic assay that employs digital recording of fluorescence. It can detect IgE levels of 5-1000 kU/L, with a coefficient of variation ranging from 2.0 to 9.5%. The assay can be pro...

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Autores principales:	Anna N Berlina, Nadezhda A Taranova, Anatoly V Zherdev, Mikhail N Sankov, Igor V Andreev, Alexandr I Martynov, Boris B Dzantiev
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/59f98cb656464b3bb81b7f829b5ab669
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spelling	oai:doaj.org-article:59f98cb656464b3bb81b7f829b5ab6692021-11-18T08:49:01ZQuantum-dot-based immunochromatographic assay for total IgE in human serum.1932-620310.1371/journal.pone.0077485https://doaj.org/article/59f98cb656464b3bb81b7f829b5ab6692013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24204841/?tool=EBIhttps://doaj.org/toc/1932-6203To rapidly quantify total immunoglobulin E levels in human serum, we developed a novel quantum-dot-based immunochromatographic assay that employs digital recording of fluorescence. It can detect IgE levels of 5-1000 kU/L, with a coefficient of variation ranging from 2.0 to 9.5%. The assay can be processed in 10 min. The developed assay was tested on 95 serum samples. The correlation coefficient between the IgE values obtained by the proposed assay and those obtained by a commercial ELISA kit was 0.9884. Our assay thus shows promise as a new diagnostic tool for IgE detection.Anna N BerlinaNadezhda A TaranovaAnatoly V ZherdevMikhail N SankovIgor V AndreevAlexandr I MartynovBoris B DzantievPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e77485 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna N Berlina Nadezhda A Taranova Anatoly V Zherdev Mikhail N Sankov Igor V Andreev Alexandr I Martynov Boris B Dzantiev Quantum-dot-based immunochromatographic assay for total IgE in human serum.
description	To rapidly quantify total immunoglobulin E levels in human serum, we developed a novel quantum-dot-based immunochromatographic assay that employs digital recording of fluorescence. It can detect IgE levels of 5-1000 kU/L, with a coefficient of variation ranging from 2.0 to 9.5%. The assay can be processed in 10 min. The developed assay was tested on 95 serum samples. The correlation coefficient between the IgE values obtained by the proposed assay and those obtained by a commercial ELISA kit was 0.9884. Our assay thus shows promise as a new diagnostic tool for IgE detection.
format	article
author	Anna N Berlina Nadezhda A Taranova Anatoly V Zherdev Mikhail N Sankov Igor V Andreev Alexandr I Martynov Boris B Dzantiev
author_facet	Anna N Berlina Nadezhda A Taranova Anatoly V Zherdev Mikhail N Sankov Igor V Andreev Alexandr I Martynov Boris B Dzantiev
author_sort	Anna N Berlina
title	Quantum-dot-based immunochromatographic assay for total IgE in human serum.
title_short	Quantum-dot-based immunochromatographic assay for total IgE in human serum.
title_full	Quantum-dot-based immunochromatographic assay for total IgE in human serum.
title_fullStr	Quantum-dot-based immunochromatographic assay for total IgE in human serum.
title_full_unstemmed	Quantum-dot-based immunochromatographic assay for total IgE in human serum.
title_sort	quantum-dot-based immunochromatographic assay for total ige in human serum.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/59f98cb656464b3bb81b7f829b5ab669
work_keys_str_mv	AT annanberlina quantumdotbasedimmunochromatographicassayfortotaligeinhumanserum AT nadezhdaataranova quantumdotbasedimmunochromatographicassayfortotaligeinhumanserum AT anatolyvzherdev quantumdotbasedimmunochromatographicassayfortotaligeinhumanserum AT mikhailnsankov quantumdotbasedimmunochromatographicassayfortotaligeinhumanserum AT igorvandreev quantumdotbasedimmunochromatographicassayfortotaligeinhumanserum AT alexandrimartynov quantumdotbasedimmunochromatographicassayfortotaligeinhumanserum AT borisbdzantiev quantumdotbasedimmunochromatographicassayfortotaligeinhumanserum
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Quantum-dot-based immunochromatographic assay for total IgE in human serum.

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