Easy ultrastructural insight into the internal morphology of biological specimens by Atomic Force Microscopy

Abstract As a topographical technique, Atomic Force Microscopy (AFM) needs to establish direct interactions between a given sample and the measurement probe in order to create imaging information. The elucidation of internal features of organisms, tissues and cells by AFM has therefore been a challe...

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Autor principal:	Fabian Christopher Herrmann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/59fdbdf25d7048c188e9dc3c19a322b4
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spelling	oai:doaj.org-article:59fdbdf25d7048c188e9dc3c19a322b42021-12-02T15:54:59ZEasy ultrastructural insight into the internal morphology of biological specimens by Atomic Force Microscopy10.1038/s41598-021-89633-22045-2322https://doaj.org/article/59fdbdf25d7048c188e9dc3c19a322b42021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89633-2https://doaj.org/toc/2045-2322Abstract As a topographical technique, Atomic Force Microscopy (AFM) needs to establish direct interactions between a given sample and the measurement probe in order to create imaging information. The elucidation of internal features of organisms, tissues and cells by AFM has therefore been a challenging process in the past. To overcome this hindrance, simple and fast embedding, sectioning and dehydration techniques are presented, allowing the easy access to the internal morphology of virtually any organism, tissue or cell by AFM. The study at hand shows the applicability of the proposed protocol to exemplary biological samples, the resolution currently allowed by the approach as well as advantages and shortcomings compared to classical ultrastructural microscopic techniques like electron microscopy. The presented cheap, facile, fast and non-toxic experimental protocol might introduce AFM as a universal tool for the elucidation of internal ultrastructural detail of virtually any given organism, tissue or cell.Fabian Christopher HerrmannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fabian Christopher Herrmann Easy ultrastructural insight into the internal morphology of biological specimens by Atomic Force Microscopy
description	Abstract As a topographical technique, Atomic Force Microscopy (AFM) needs to establish direct interactions between a given sample and the measurement probe in order to create imaging information. The elucidation of internal features of organisms, tissues and cells by AFM has therefore been a challenging process in the past. To overcome this hindrance, simple and fast embedding, sectioning and dehydration techniques are presented, allowing the easy access to the internal morphology of virtually any organism, tissue or cell by AFM. The study at hand shows the applicability of the proposed protocol to exemplary biological samples, the resolution currently allowed by the approach as well as advantages and shortcomings compared to classical ultrastructural microscopic techniques like electron microscopy. The presented cheap, facile, fast and non-toxic experimental protocol might introduce AFM as a universal tool for the elucidation of internal ultrastructural detail of virtually any given organism, tissue or cell.
format	article
author	Fabian Christopher Herrmann
author_facet	Fabian Christopher Herrmann
author_sort	Fabian Christopher Herrmann
title	Easy ultrastructural insight into the internal morphology of biological specimens by Atomic Force Microscopy
title_short	Easy ultrastructural insight into the internal morphology of biological specimens by Atomic Force Microscopy
title_full	Easy ultrastructural insight into the internal morphology of biological specimens by Atomic Force Microscopy
title_fullStr	Easy ultrastructural insight into the internal morphology of biological specimens by Atomic Force Microscopy
title_full_unstemmed	Easy ultrastructural insight into the internal morphology of biological specimens by Atomic Force Microscopy
title_sort	easy ultrastructural insight into the internal morphology of biological specimens by atomic force microscopy
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/59fdbdf25d7048c188e9dc3c19a322b4
work_keys_str_mv	AT fabianchristopherherrmann easyultrastructuralinsightintotheinternalmorphologyofbiologicalspecimensbyatomicforcemicroscopy
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Easy ultrastructural insight into the internal morphology of biological specimens by Atomic Force Microscopy

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