A variety of hydrogenotrophic enrichment cultures catalyse cathodic reactions

Abstract Biocathodes where living microorganisms catalyse reduction of CO2 can potentially be used to produce valuable chemicals. Microorganisms harbouring hydrogenases may play a key role for biocathode performance since H2 generated on the electrode surface can act as an electron donor for CO2 red...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Soroush Saheb-Alam, Frank Persson, Britt-Marie Wilén, Malte Hermansson, Oskar Modin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5a00499735d84363a364a6312b801d9b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:5a00499735d84363a364a6312b801d9b
record_format	dspace
spelling	oai:doaj.org-article:5a00499735d84363a364a6312b801d9b2021-12-02T16:08:27ZA variety of hydrogenotrophic enrichment cultures catalyse cathodic reactions10.1038/s41598-018-38006-32045-2322https://doaj.org/article/5a00499735d84363a364a6312b801d9b2019-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-38006-3https://doaj.org/toc/2045-2322Abstract Biocathodes where living microorganisms catalyse reduction of CO2 can potentially be used to produce valuable chemicals. Microorganisms harbouring hydrogenases may play a key role for biocathode performance since H2 generated on the electrode surface can act as an electron donor for CO2 reduction. In this study, the possibility of catalysing cathodic reactions by hydrogenotrophic methanogens, acetogens, sulfate-reducers, denitrifiers, and acetotrophic methanogens was investigated. The cultures were enriched from an activated sludge inoculum and performed the expected metabolic functions. All enrichments formed distinct microbial communities depending on their electron donor and electron acceptor. When the cultures were added to an electrochemical cell, linear sweep voltammograms showed a shift in current generation close to the hydrogen evolution potential (−1 V versus SHE) with higher cathodic current produced at a more positive potential. All enrichment cultures except the denitrifiers were also used to inoculate biocathodes of microbial electrolysis cells operated with H+ and bicarbonate as electron acceptors and this resulted in current densities between 0.1–1 A/m2. The microbial community composition of biocathodes inoculated with different enrichment cultures were as different from each other as they were different from their suspended culture inoculum. It was noteworthy that Methanobacterium sp. appeared on all the biocathodes suggesting that it is a key microorganism catalysing biocathode reactions.Soroush Saheb-AlamFrank PerssonBritt-Marie WilénMalte HermanssonOskar ModinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Soroush Saheb-Alam Frank Persson Britt-Marie Wilén Malte Hermansson Oskar Modin A variety of hydrogenotrophic enrichment cultures catalyse cathodic reactions
description	Abstract Biocathodes where living microorganisms catalyse reduction of CO2 can potentially be used to produce valuable chemicals. Microorganisms harbouring hydrogenases may play a key role for biocathode performance since H2 generated on the electrode surface can act as an electron donor for CO2 reduction. In this study, the possibility of catalysing cathodic reactions by hydrogenotrophic methanogens, acetogens, sulfate-reducers, denitrifiers, and acetotrophic methanogens was investigated. The cultures were enriched from an activated sludge inoculum and performed the expected metabolic functions. All enrichments formed distinct microbial communities depending on their electron donor and electron acceptor. When the cultures were added to an electrochemical cell, linear sweep voltammograms showed a shift in current generation close to the hydrogen evolution potential (−1 V versus SHE) with higher cathodic current produced at a more positive potential. All enrichment cultures except the denitrifiers were also used to inoculate biocathodes of microbial electrolysis cells operated with H+ and bicarbonate as electron acceptors and this resulted in current densities between 0.1–1 A/m2. The microbial community composition of biocathodes inoculated with different enrichment cultures were as different from each other as they were different from their suspended culture inoculum. It was noteworthy that Methanobacterium sp. appeared on all the biocathodes suggesting that it is a key microorganism catalysing biocathode reactions.
format	article
author	Soroush Saheb-Alam Frank Persson Britt-Marie Wilén Malte Hermansson Oskar Modin
author_facet	Soroush Saheb-Alam Frank Persson Britt-Marie Wilén Malte Hermansson Oskar Modin
author_sort	Soroush Saheb-Alam
title	A variety of hydrogenotrophic enrichment cultures catalyse cathodic reactions
title_short	A variety of hydrogenotrophic enrichment cultures catalyse cathodic reactions
title_full	A variety of hydrogenotrophic enrichment cultures catalyse cathodic reactions
title_fullStr	A variety of hydrogenotrophic enrichment cultures catalyse cathodic reactions
title_full_unstemmed	A variety of hydrogenotrophic enrichment cultures catalyse cathodic reactions
title_sort	variety of hydrogenotrophic enrichment cultures catalyse cathodic reactions
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/5a00499735d84363a364a6312b801d9b
work_keys_str_mv	AT soroushsahebalam avarietyofhydrogenotrophicenrichmentculturescatalysecathodicreactions AT frankpersson avarietyofhydrogenotrophicenrichmentculturescatalysecathodicreactions AT brittmariewilen avarietyofhydrogenotrophicenrichmentculturescatalysecathodicreactions AT maltehermansson avarietyofhydrogenotrophicenrichmentculturescatalysecathodicreactions AT oskarmodin avarietyofhydrogenotrophicenrichmentculturescatalysecathodicreactions AT soroushsahebalam varietyofhydrogenotrophicenrichmentculturescatalysecathodicreactions AT frankpersson varietyofhydrogenotrophicenrichmentculturescatalysecathodicreactions AT brittmariewilen varietyofhydrogenotrophicenrichmentculturescatalysecathodicreactions AT maltehermansson varietyofhydrogenotrophicenrichmentculturescatalysecathodicreactions AT oskarmodin varietyofhydrogenotrophicenrichmentculturescatalysecathodicreactions
_version_	1718384470066724864

A variety of hydrogenotrophic enrichment cultures catalyse cathodic reactions

Ejemplares similares