New emerging entomopathogenic fungi isolated from soil in South Sumatra (Indonesia) and their filtrate and conidial insecticidal activity against Spodoptera litura

Abstract. Herlinda S, Efendi RA, Suharjo R, Hasbi, Setiawan A, Elfita, Verawaty M. 2020. New emerging entomopathogenic fungi isolated from soil in South Sumatra (Indonesia) and their filtrate and conidial insecticidal activity against Spodoptera litura. Biodiversitas 21: 5102-5113. This study was ca...

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Autores principales:	Siti Herlinda, Riski Anwar Efendi, Radix Suharjo, Hasbi Hasbi, Arum Setiawan, Elfita Elfita, Marieska Verawaty
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	beauveria bassiana, leaf-eating caterpillar, metarhizium anisopliae, penicillium citrinum, talaromyces diversus Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/5a04522c48b94a0e963a11ba1f78ef8d
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spelling	oai:doaj.org-article:5a04522c48b94a0e963a11ba1f78ef8d2021-11-22T00:44:11ZNew emerging entomopathogenic fungi isolated from soil in South Sumatra (Indonesia) and their filtrate and conidial insecticidal activity against Spodoptera litura1412-033X2085-472210.13057/biodiv/d211115https://doaj.org/article/5a04522c48b94a0e963a11ba1f78ef8d2020-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6815https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Herlinda S, Efendi RA, Suharjo R, Hasbi, Setiawan A, Elfita, Verawaty M. 2020. New emerging entomopathogenic fungi isolated from soil in South Sumatra (Indonesia) and their filtrate and conidial insecticidal activity against Spodoptera litura. Biodiversitas 21: 5102-5113. This study was carried out to confirm identity of Beauveria bassiana and Metarhizium anisopliae using sequence analysis of Internal Transcribed Spacer (ITS) region as well as the two unknown isolates of EPF and to investigate their filtrate and conidial insecticidal activity against S. litura larvae. The EPF species were identified based on morphological characters and molecular analysis. Their culture filtrate and conidial bioassays were performed on S. litura larvae. The species found were Penicillium citrinum (BKbTp), Talaromyces diversus (MSwTp1), B. bassiana (BSwTd4), and M. anisopliae (MSwTp3) and have been deposited in the GenBank. All EPF species have high insecticidal activity against S. litura larvae. The highest larvae mortality caused by filtrate was found on P. citrinum (98.67%) and not significantly different from those of T. diversus (96%), but significantly different from B. bassiana (86.67%) and M anisopliae (82.67%). The highest larvae mortality caused by the conidia was also occurred to P. citrinum (98.67%) which was not significantly different from those of T. diversus (97.33%), B. bassiana (86.67%), and M. anisopliae (86.67%). The LT50 caused by T. diversus filtrate was the shortest (6.19 days) and not significantly different from those caused by P. citrinum (6.62 days). The shortest LT50 caused by conidia was found on P. citrinum (5.45 days). Therefore, the conidia and filtrate of P. citrinum, a new emerging entomopathogenic fungus, has the highest insecticidal activity. P. citrinum has the potential to be developed into commercial mycoinsecticides.Siti HerlindaRiski Anwar EfendiRadix SuharjoHasbi HasbiArum SetiawanElfita ElfitaMarieska VerawatyMBI & UNS Soloarticlebeauveria bassiana, leaf-eating caterpillar, metarhizium anisopliae, penicillium citrinum, talaromyces diversusBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	beauveria bassiana, leaf-eating caterpillar, metarhizium anisopliae, penicillium citrinum, talaromyces diversus Biology (General) QH301-705.5
spellingShingle	beauveria bassiana, leaf-eating caterpillar, metarhizium anisopliae, penicillium citrinum, talaromyces diversus Biology (General) QH301-705.5 Siti Herlinda Riski Anwar Efendi Radix Suharjo Hasbi Hasbi Arum Setiawan Elfita Elfita Marieska Verawaty New emerging entomopathogenic fungi isolated from soil in South Sumatra (Indonesia) and their filtrate and conidial insecticidal activity against Spodoptera litura
description	Abstract. Herlinda S, Efendi RA, Suharjo R, Hasbi, Setiawan A, Elfita, Verawaty M. 2020. New emerging entomopathogenic fungi isolated from soil in South Sumatra (Indonesia) and their filtrate and conidial insecticidal activity against Spodoptera litura. Biodiversitas 21: 5102-5113. This study was carried out to confirm identity of Beauveria bassiana and Metarhizium anisopliae using sequence analysis of Internal Transcribed Spacer (ITS) region as well as the two unknown isolates of EPF and to investigate their filtrate and conidial insecticidal activity against S. litura larvae. The EPF species were identified based on morphological characters and molecular analysis. Their culture filtrate and conidial bioassays were performed on S. litura larvae. The species found were Penicillium citrinum (BKbTp), Talaromyces diversus (MSwTp1), B. bassiana (BSwTd4), and M. anisopliae (MSwTp3) and have been deposited in the GenBank. All EPF species have high insecticidal activity against S. litura larvae. The highest larvae mortality caused by filtrate was found on P. citrinum (98.67%) and not significantly different from those of T. diversus (96%), but significantly different from B. bassiana (86.67%) and M anisopliae (82.67%). The highest larvae mortality caused by the conidia was also occurred to P. citrinum (98.67%) which was not significantly different from those of T. diversus (97.33%), B. bassiana (86.67%), and M. anisopliae (86.67%). The LT50 caused by T. diversus filtrate was the shortest (6.19 days) and not significantly different from those caused by P. citrinum (6.62 days). The shortest LT50 caused by conidia was found on P. citrinum (5.45 days). Therefore, the conidia and filtrate of P. citrinum, a new emerging entomopathogenic fungus, has the highest insecticidal activity. P. citrinum has the potential to be developed into commercial mycoinsecticides.
format	article
author	Siti Herlinda Riski Anwar Efendi Radix Suharjo Hasbi Hasbi Arum Setiawan Elfita Elfita Marieska Verawaty
author_facet	Siti Herlinda Riski Anwar Efendi Radix Suharjo Hasbi Hasbi Arum Setiawan Elfita Elfita Marieska Verawaty
author_sort	Siti Herlinda
title	New emerging entomopathogenic fungi isolated from soil in South Sumatra (Indonesia) and their filtrate and conidial insecticidal activity against Spodoptera litura
title_short	New emerging entomopathogenic fungi isolated from soil in South Sumatra (Indonesia) and their filtrate and conidial insecticidal activity against Spodoptera litura
title_full	New emerging entomopathogenic fungi isolated from soil in South Sumatra (Indonesia) and their filtrate and conidial insecticidal activity against Spodoptera litura
title_fullStr	New emerging entomopathogenic fungi isolated from soil in South Sumatra (Indonesia) and their filtrate and conidial insecticidal activity against Spodoptera litura
title_full_unstemmed	New emerging entomopathogenic fungi isolated from soil in South Sumatra (Indonesia) and their filtrate and conidial insecticidal activity against Spodoptera litura
title_sort	new emerging entomopathogenic fungi isolated from soil in south sumatra (indonesia) and their filtrate and conidial insecticidal activity against spodoptera litura
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/5a04522c48b94a0e963a11ba1f78ef8d
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New emerging entomopathogenic fungi isolated from soil in South Sumatra (Indonesia) and their filtrate and conidial insecticidal activity against Spodoptera litura

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