Identification and application of exogenous dsRNA confers plant protection against Sclerotinia sclerotiorum and Botrytis cinerea

Abstract Sclerotinia sclerotiorum, the causal agent of white stem rot, is responsible for significant losses in crop yields around the globe. While our understanding of S. sclerotiorum infection is becoming clearer, genetic control of the pathogen has been elusive and effective control of pathogen c...

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Autores principales:	Austein G. McLoughlin, Nick Wytinck, Philip L. Walker, Ian J. Girard, Khalid Y. Rashid, Teresa de Kievit, W. G. Dilantha Fernando, Steve Whyard, Mark F. Belmonte
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5a06131e1a014bbf98bc01fdad655c1b
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spelling	oai:doaj.org-article:5a06131e1a014bbf98bc01fdad655c1b2021-12-02T15:05:02ZIdentification and application of exogenous dsRNA confers plant protection against Sclerotinia sclerotiorum and Botrytis cinerea10.1038/s41598-018-25434-42045-2322https://doaj.org/article/5a06131e1a014bbf98bc01fdad655c1b2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25434-4https://doaj.org/toc/2045-2322Abstract Sclerotinia sclerotiorum, the causal agent of white stem rot, is responsible for significant losses in crop yields around the globe. While our understanding of S. sclerotiorum infection is becoming clearer, genetic control of the pathogen has been elusive and effective control of pathogen colonization using traditional broad-spectrum agro-chemical protocols are less effective than desired. In the current study, we developed species-specific RNA interference-based control treatments capable of reducing fungal infection. Development of a target identification pipeline using global RNA sequencing data for selection and application of double stranded RNA (dsRNA) molecules identified single gene targets of the fungus. Using this approach, we demonstrate the utility of this technology through foliar applications of dsRNAs to the leaf surface that significantly decreased fungal infection and S. sclerotiorum disease symptoms. Select target gene homologs were also tested in the closely related species, Botrytis cinerea, reducing lesion size and providing compelling evidence of the adaptability and flexibility of this technology in protecting plants against devastating fungal pathogens.Austein G. McLoughlinNick WytinckPhilip L. WalkerIan J. GirardKhalid Y. RashidTeresa de KievitW. G. Dilantha FernandoSteve WhyardMark F. BelmonteNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Austein G. McLoughlin Nick Wytinck Philip L. Walker Ian J. Girard Khalid Y. Rashid Teresa de Kievit W. G. Dilantha Fernando Steve Whyard Mark F. Belmonte Identification and application of exogenous dsRNA confers plant protection against Sclerotinia sclerotiorum and Botrytis cinerea
description	Abstract Sclerotinia sclerotiorum, the causal agent of white stem rot, is responsible for significant losses in crop yields around the globe. While our understanding of S. sclerotiorum infection is becoming clearer, genetic control of the pathogen has been elusive and effective control of pathogen colonization using traditional broad-spectrum agro-chemical protocols are less effective than desired. In the current study, we developed species-specific RNA interference-based control treatments capable of reducing fungal infection. Development of a target identification pipeline using global RNA sequencing data for selection and application of double stranded RNA (dsRNA) molecules identified single gene targets of the fungus. Using this approach, we demonstrate the utility of this technology through foliar applications of dsRNAs to the leaf surface that significantly decreased fungal infection and S. sclerotiorum disease symptoms. Select target gene homologs were also tested in the closely related species, Botrytis cinerea, reducing lesion size and providing compelling evidence of the adaptability and flexibility of this technology in protecting plants against devastating fungal pathogens.
format	article
author	Austein G. McLoughlin Nick Wytinck Philip L. Walker Ian J. Girard Khalid Y. Rashid Teresa de Kievit W. G. Dilantha Fernando Steve Whyard Mark F. Belmonte
author_facet	Austein G. McLoughlin Nick Wytinck Philip L. Walker Ian J. Girard Khalid Y. Rashid Teresa de Kievit W. G. Dilantha Fernando Steve Whyard Mark F. Belmonte
author_sort	Austein G. McLoughlin
title	Identification and application of exogenous dsRNA confers plant protection against Sclerotinia sclerotiorum and Botrytis cinerea
title_short	Identification and application of exogenous dsRNA confers plant protection against Sclerotinia sclerotiorum and Botrytis cinerea
title_full	Identification and application of exogenous dsRNA confers plant protection against Sclerotinia sclerotiorum and Botrytis cinerea
title_fullStr	Identification and application of exogenous dsRNA confers plant protection against Sclerotinia sclerotiorum and Botrytis cinerea
title_full_unstemmed	Identification and application of exogenous dsRNA confers plant protection against Sclerotinia sclerotiorum and Botrytis cinerea
title_sort	identification and application of exogenous dsrna confers plant protection against sclerotinia sclerotiorum and botrytis cinerea
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/5a06131e1a014bbf98bc01fdad655c1b
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Identification and application of exogenous dsRNA confers plant protection against Sclerotinia sclerotiorum and Botrytis cinerea

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