Efficacy and immunogenicity of different BCG doses in BALB/c and CB6F1 mice when challenged with H37Rv or Beijing HN878
Abstract Two strains of mice (BALB/c and CB6F1) were vaccinated with a range of Bacille Calmette-Guérin (BCG) Danish doses from 3 × 105 to 30 CFU/mouse, followed by aerosol infection with Mtb (H37Rv or West-Beijing HN878 strain). The results indicated that both strains of mice when infected with HN8...
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Nature Portfolio
2021
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oai:doaj.org-article:5a06714d4d7341239fefee3d1d60308b2021-12-05T12:13:11ZEfficacy and immunogenicity of different BCG doses in BALB/c and CB6F1 mice when challenged with H37Rv or Beijing HN87810.1038/s41598-021-02442-52045-2322https://doaj.org/article/5a06714d4d7341239fefee3d1d60308b2021-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02442-5https://doaj.org/toc/2045-2322Abstract Two strains of mice (BALB/c and CB6F1) were vaccinated with a range of Bacille Calmette-Guérin (BCG) Danish doses from 3 × 105 to 30 CFU/mouse, followed by aerosol infection with Mtb (H37Rv or West-Beijing HN878 strain). The results indicated that both strains of mice when infected with HN878 exhibited significant protection in their lungs with BCG doses at 3 × 105—3000 CFU (BALB/c) and 3 × 105—300 CFU (CB6F1). Whereas, a significant protection was seen in both strains of mice with BCG doses at 3 × 105—300 CFU when infected with H37Rv. A significant increase in the frequencies of BCG-specific IFNγ+ IL2+ TNFα+ CD4 T cells in the BCG doses at 3 × 105—3000 CFU (BALB/c) and 3 × 105—300 CFU (CB6F1) was seen. The IFNγ+ IL2+ TNFα+ CD4 T cells correlated with the Mtb burden in the lungs of HN878 infected mice (BALB/c and CB6F1) whereas, IFNγ+ TNFα+ CD4 T cells correlated with the BALB/c mice infected with H37Rv or HN878. The BCG dose at 3000 CFU (an equivalent single human dose in the mice by body weight) is protective in both strains of mice infected with H37Rv or HN878 and may serve an interesting dose to test new TB vaccine in a preclinical animal model.Bhagwati KhatriJames KeebleBelinda DaggDaryan A. KavehPhilip J. HogarthMei Mei HoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) |
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EN |
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Medicine R Science Q |
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Medicine R Science Q Bhagwati Khatri James Keeble Belinda Dagg Daryan A. Kaveh Philip J. Hogarth Mei Mei Ho Efficacy and immunogenicity of different BCG doses in BALB/c and CB6F1 mice when challenged with H37Rv or Beijing HN878 |
| description |
Abstract Two strains of mice (BALB/c and CB6F1) were vaccinated with a range of Bacille Calmette-Guérin (BCG) Danish doses from 3 × 105 to 30 CFU/mouse, followed by aerosol infection with Mtb (H37Rv or West-Beijing HN878 strain). The results indicated that both strains of mice when infected with HN878 exhibited significant protection in their lungs with BCG doses at 3 × 105—3000 CFU (BALB/c) and 3 × 105—300 CFU (CB6F1). Whereas, a significant protection was seen in both strains of mice with BCG doses at 3 × 105—300 CFU when infected with H37Rv. A significant increase in the frequencies of BCG-specific IFNγ+ IL2+ TNFα+ CD4 T cells in the BCG doses at 3 × 105—3000 CFU (BALB/c) and 3 × 105—300 CFU (CB6F1) was seen. The IFNγ+ IL2+ TNFα+ CD4 T cells correlated with the Mtb burden in the lungs of HN878 infected mice (BALB/c and CB6F1) whereas, IFNγ+ TNFα+ CD4 T cells correlated with the BALB/c mice infected with H37Rv or HN878. The BCG dose at 3000 CFU (an equivalent single human dose in the mice by body weight) is protective in both strains of mice infected with H37Rv or HN878 and may serve an interesting dose to test new TB vaccine in a preclinical animal model. |
| format |
article |
| author |
Bhagwati Khatri James Keeble Belinda Dagg Daryan A. Kaveh Philip J. Hogarth Mei Mei Ho |
| author_facet |
Bhagwati Khatri James Keeble Belinda Dagg Daryan A. Kaveh Philip J. Hogarth Mei Mei Ho |
| author_sort |
Bhagwati Khatri |
| title |
Efficacy and immunogenicity of different BCG doses in BALB/c and CB6F1 mice when challenged with H37Rv or Beijing HN878 |
| title_short |
Efficacy and immunogenicity of different BCG doses in BALB/c and CB6F1 mice when challenged with H37Rv or Beijing HN878 |
| title_full |
Efficacy and immunogenicity of different BCG doses in BALB/c and CB6F1 mice when challenged with H37Rv or Beijing HN878 |
| title_fullStr |
Efficacy and immunogenicity of different BCG doses in BALB/c and CB6F1 mice when challenged with H37Rv or Beijing HN878 |
| title_full_unstemmed |
Efficacy and immunogenicity of different BCG doses in BALB/c and CB6F1 mice when challenged with H37Rv or Beijing HN878 |
| title_sort |
efficacy and immunogenicity of different bcg doses in balb/c and cb6f1 mice when challenged with h37rv or beijing hn878 |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/5a06714d4d7341239fefee3d1d60308b |
| work_keys_str_mv |
AT bhagwatikhatri efficacyandimmunogenicityofdifferentbcgdosesinbalbcandcb6f1micewhenchallengedwithh37rvorbeijinghn878 AT jameskeeble efficacyandimmunogenicityofdifferentbcgdosesinbalbcandcb6f1micewhenchallengedwithh37rvorbeijinghn878 AT belindadagg efficacyandimmunogenicityofdifferentbcgdosesinbalbcandcb6f1micewhenchallengedwithh37rvorbeijinghn878 AT daryanakaveh efficacyandimmunogenicityofdifferentbcgdosesinbalbcandcb6f1micewhenchallengedwithh37rvorbeijinghn878 AT philipjhogarth efficacyandimmunogenicityofdifferentbcgdosesinbalbcandcb6f1micewhenchallengedwithh37rvorbeijinghn878 AT meimeiho efficacyandimmunogenicityofdifferentbcgdosesinbalbcandcb6f1micewhenchallengedwithh37rvorbeijinghn878 |
| _version_ |
1718372133882560512 |