Elicitation of both anti HIV-1 Env humoral and cellular immunities by replicating vaccinia prime Sendai virus boost regimen and boosting by CD40Lm.

For protection from HIV-1 infection, a vaccine should elicit both humoral and cell-mediated immune responses. A novel vaccine regimen and adjuvant that induce high levels of HIV-1 Env-specific T cell and antibody (Ab) responses was developed in this study. The prime-boost regimen that used combinati...

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Autores principales: Xianfeng Zhang, Tomoyoshi Sobue, Mao Isshiki, Shun-ichi Makino, Makoto Inoue, Kazunori Kato, Tatsuo Shioda, Takashi Ohashi, Hirotaka Sato, Jun Komano, Hideji Hanabusa, Hisatoshi Shida
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Publicado: Public Library of Science (PLoS) 2012
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Acceso en línea:https://doaj.org/article/5a08476b99af474485dd29d8030cd44b
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spelling oai:doaj.org-article:5a08476b99af474485dd29d8030cd44b2021-11-18T08:05:53ZElicitation of both anti HIV-1 Env humoral and cellular immunities by replicating vaccinia prime Sendai virus boost regimen and boosting by CD40Lm.1932-620310.1371/journal.pone.0051633https://doaj.org/article/5a08476b99af474485dd29d8030cd44b2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23236521/?tool=EBIhttps://doaj.org/toc/1932-6203For protection from HIV-1 infection, a vaccine should elicit both humoral and cell-mediated immune responses. A novel vaccine regimen and adjuvant that induce high levels of HIV-1 Env-specific T cell and antibody (Ab) responses was developed in this study. The prime-boost regimen that used combinations of replication-competent vaccinia LC16m8Δ (m8Δ) and Sendai virus (SeV) vectors expressing HIV-1 Env efficiently produced both Env-specific CD8(+) T cells and anti-Env antibodies, including neutralizing antibodies (nAbs). These results sharply contrast with vaccine regimens that prime with an Env expressing plasmid and boost with the m8Δ or SeV vector that mainly elicited cellular immunities. Moreover, co-priming with combinations of m8Δs expressing Env or a membrane-bound human CD40 ligand mutant (CD40Lm) enhanced Env-specific CD8(+) T cell production, but not anti-Env antibody production. In contrast, priming with an m8Δ that coexpresses CD40Lm and Env elicited more anti-Env Abs with higher avidity, but did not promote T cell responses. These results suggest that the m8Δ prime/SeV boost regimen in conjunction with CD40Lm expression could be used as an immunization platform for driving both potent cellular and humoral immunities against pathogens such as HIV-1.Xianfeng ZhangTomoyoshi SobueMao IsshikiShun-ichi MakinoMakoto InoueKazunori KatoTatsuo ShiodaTakashi OhashiHirotaka SatoJun KomanoHideji HanabusaHisatoshi ShidaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e51633 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Xianfeng Zhang
Tomoyoshi Sobue
Mao Isshiki
Shun-ichi Makino
Makoto Inoue
Kazunori Kato
Tatsuo Shioda
Takashi Ohashi
Hirotaka Sato
Jun Komano
Hideji Hanabusa
Hisatoshi Shida
Elicitation of both anti HIV-1 Env humoral and cellular immunities by replicating vaccinia prime Sendai virus boost regimen and boosting by CD40Lm.
description For protection from HIV-1 infection, a vaccine should elicit both humoral and cell-mediated immune responses. A novel vaccine regimen and adjuvant that induce high levels of HIV-1 Env-specific T cell and antibody (Ab) responses was developed in this study. The prime-boost regimen that used combinations of replication-competent vaccinia LC16m8Δ (m8Δ) and Sendai virus (SeV) vectors expressing HIV-1 Env efficiently produced both Env-specific CD8(+) T cells and anti-Env antibodies, including neutralizing antibodies (nAbs). These results sharply contrast with vaccine regimens that prime with an Env expressing plasmid and boost with the m8Δ or SeV vector that mainly elicited cellular immunities. Moreover, co-priming with combinations of m8Δs expressing Env or a membrane-bound human CD40 ligand mutant (CD40Lm) enhanced Env-specific CD8(+) T cell production, but not anti-Env antibody production. In contrast, priming with an m8Δ that coexpresses CD40Lm and Env elicited more anti-Env Abs with higher avidity, but did not promote T cell responses. These results suggest that the m8Δ prime/SeV boost regimen in conjunction with CD40Lm expression could be used as an immunization platform for driving both potent cellular and humoral immunities against pathogens such as HIV-1.
format article
author Xianfeng Zhang
Tomoyoshi Sobue
Mao Isshiki
Shun-ichi Makino
Makoto Inoue
Kazunori Kato
Tatsuo Shioda
Takashi Ohashi
Hirotaka Sato
Jun Komano
Hideji Hanabusa
Hisatoshi Shida
author_facet Xianfeng Zhang
Tomoyoshi Sobue
Mao Isshiki
Shun-ichi Makino
Makoto Inoue
Kazunori Kato
Tatsuo Shioda
Takashi Ohashi
Hirotaka Sato
Jun Komano
Hideji Hanabusa
Hisatoshi Shida
author_sort Xianfeng Zhang
title Elicitation of both anti HIV-1 Env humoral and cellular immunities by replicating vaccinia prime Sendai virus boost regimen and boosting by CD40Lm.
title_short Elicitation of both anti HIV-1 Env humoral and cellular immunities by replicating vaccinia prime Sendai virus boost regimen and boosting by CD40Lm.
title_full Elicitation of both anti HIV-1 Env humoral and cellular immunities by replicating vaccinia prime Sendai virus boost regimen and boosting by CD40Lm.
title_fullStr Elicitation of both anti HIV-1 Env humoral and cellular immunities by replicating vaccinia prime Sendai virus boost regimen and boosting by CD40Lm.
title_full_unstemmed Elicitation of both anti HIV-1 Env humoral and cellular immunities by replicating vaccinia prime Sendai virus boost regimen and boosting by CD40Lm.
title_sort elicitation of both anti hiv-1 env humoral and cellular immunities by replicating vaccinia prime sendai virus boost regimen and boosting by cd40lm.
publisher Public Library of Science (PLoS)
publishDate 2012
url https://doaj.org/article/5a08476b99af474485dd29d8030cd44b
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