The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy

Abstract Cowpea mosaic virus (CPMV) is a picorna-like plant virus. As well as an intrinsic interest in CPMV as a plant pathogen, CPMV is of major interest in biotechnology applications such as nanotechnology. Here, we report high resolution cryo electron microscopy (cryo-EM) maps of wild type CPMV c...

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Autores principales:	Emma L. Hesketh, Yulia Meshcheriakova, Rebecca F. Thompson, George P. Lomonossoff, Neil A. Ranson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5a08a9447f2247ffa6187b87a1890e27
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spelling	oai:doaj.org-article:5a08a9447f2247ffa6187b87a1890e272021-12-02T11:52:27ZThe structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy10.1038/s41598-017-00533-w2045-2322https://doaj.org/article/5a08a9447f2247ffa6187b87a1890e272017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00533-whttps://doaj.org/toc/2045-2322Abstract Cowpea mosaic virus (CPMV) is a picorna-like plant virus. As well as an intrinsic interest in CPMV as a plant pathogen, CPMV is of major interest in biotechnology applications such as nanotechnology. Here, we report high resolution cryo electron microscopy (cryo-EM) maps of wild type CPMV containing RNA-2, and of naturally-formed empty CPMV capsids. The resolution of these structures is sufficient to visualise large amino acids. We have refined an atomic model for each map and identified an essential amino acid involved in genome encapsidation. This work has furthered our knowledge of Picornavirales genome encapsidation and will assist further work in the development of CPMV as a biotechnological tool.Emma L. HeskethYulia MeshcheriakovaRebecca F. ThompsonGeorge P. LomonossoffNeil A. RansonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Emma L. Hesketh Yulia Meshcheriakova Rebecca F. Thompson George P. Lomonossoff Neil A. Ranson The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy
description	Abstract Cowpea mosaic virus (CPMV) is a picorna-like plant virus. As well as an intrinsic interest in CPMV as a plant pathogen, CPMV is of major interest in biotechnology applications such as nanotechnology. Here, we report high resolution cryo electron microscopy (cryo-EM) maps of wild type CPMV containing RNA-2, and of naturally-formed empty CPMV capsids. The resolution of these structures is sufficient to visualise large amino acids. We have refined an atomic model for each map and identified an essential amino acid involved in genome encapsidation. This work has furthered our knowledge of Picornavirales genome encapsidation and will assist further work in the development of CPMV as a biotechnological tool.
format	article
author	Emma L. Hesketh Yulia Meshcheriakova Rebecca F. Thompson George P. Lomonossoff Neil A. Ranson
author_facet	Emma L. Hesketh Yulia Meshcheriakova Rebecca F. Thompson George P. Lomonossoff Neil A. Ranson
author_sort	Emma L. Hesketh
title	The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy
title_short	The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy
title_full	The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy
title_fullStr	The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy
title_full_unstemmed	The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy
title_sort	structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5a08a9447f2247ffa6187b87a1890e27
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The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy

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