Precise spatio-temporal control of rapid optogenetic cell ablation with mem-KillerRed in Zebrafish

Abstract The ability to kill individual or groups of cells in vivo is important for studying cellular processes and their physiological function. Cell-specific genetically encoded photosensitizing proteins, such as KillerRed, permit spatiotemporal optogenetic ablation with low-power laser light. We...

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Autores principales:	C. Buckley, M. T. Carvalho, L. K. Young, S. A. Rider, C. McFadden, C. Berlage, R. F. Verdon, J. M. Taylor, J. M. Girkin, J. J. Mullins
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5a0a1d98f432490280e51841d44c464a
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spelling	oai:doaj.org-article:5a0a1d98f432490280e51841d44c464a2021-12-02T11:50:56ZPrecise spatio-temporal control of rapid optogenetic cell ablation with mem-KillerRed in Zebrafish10.1038/s41598-017-05028-22045-2322https://doaj.org/article/5a0a1d98f432490280e51841d44c464a2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05028-2https://doaj.org/toc/2045-2322Abstract The ability to kill individual or groups of cells in vivo is important for studying cellular processes and their physiological function. Cell-specific genetically encoded photosensitizing proteins, such as KillerRed, permit spatiotemporal optogenetic ablation with low-power laser light. We report dramatically improved resolution and speed of cell targeting in the zebrafish kidney through the use of a selective plane illumination microscope (SPIM). Furthermore, through the novel incorporation of a Bessel beam into the SPIM imaging arm, we were able to improve on targeting speed and precision. The low diffraction of the Bessel beam coupled with the ability to tightly focus it through a high NA lens allowed precise, rapid targeting of subsets of cells at anatomical depth in live, developing zebrafish kidneys. We demonstrate that these specific targeting strategies significantly increase the speed of optoablation as well as fish survival.C. BuckleyM. T. CarvalhoL. K. YoungS. A. RiderC. McFaddenC. BerlageR. F. VerdonJ. M. TaylorJ. M. GirkinJ. J. MullinsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q C. Buckley M. T. Carvalho L. K. Young S. A. Rider C. McFadden C. Berlage R. F. Verdon J. M. Taylor J. M. Girkin J. J. Mullins Precise spatio-temporal control of rapid optogenetic cell ablation with mem-KillerRed in Zebrafish
description	Abstract The ability to kill individual or groups of cells in vivo is important for studying cellular processes and their physiological function. Cell-specific genetically encoded photosensitizing proteins, such as KillerRed, permit spatiotemporal optogenetic ablation with low-power laser light. We report dramatically improved resolution and speed of cell targeting in the zebrafish kidney through the use of a selective plane illumination microscope (SPIM). Furthermore, through the novel incorporation of a Bessel beam into the SPIM imaging arm, we were able to improve on targeting speed and precision. The low diffraction of the Bessel beam coupled with the ability to tightly focus it through a high NA lens allowed precise, rapid targeting of subsets of cells at anatomical depth in live, developing zebrafish kidneys. We demonstrate that these specific targeting strategies significantly increase the speed of optoablation as well as fish survival.
format	article
author	C. Buckley M. T. Carvalho L. K. Young S. A. Rider C. McFadden C. Berlage R. F. Verdon J. M. Taylor J. M. Girkin J. J. Mullins
author_facet	C. Buckley M. T. Carvalho L. K. Young S. A. Rider C. McFadden C. Berlage R. F. Verdon J. M. Taylor J. M. Girkin J. J. Mullins
author_sort	C. Buckley
title	Precise spatio-temporal control of rapid optogenetic cell ablation with mem-KillerRed in Zebrafish
title_short	Precise spatio-temporal control of rapid optogenetic cell ablation with mem-KillerRed in Zebrafish
title_full	Precise spatio-temporal control of rapid optogenetic cell ablation with mem-KillerRed in Zebrafish
title_fullStr	Precise spatio-temporal control of rapid optogenetic cell ablation with mem-KillerRed in Zebrafish
title_full_unstemmed	Precise spatio-temporal control of rapid optogenetic cell ablation with mem-KillerRed in Zebrafish
title_sort	precise spatio-temporal control of rapid optogenetic cell ablation with mem-killerred in zebrafish
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/5a0a1d98f432490280e51841d44c464a
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Precise spatio-temporal control of rapid optogenetic cell ablation with mem-KillerRed in Zebrafish

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