Matrix effect of swine urine on time-resolved fluorescent nanobeads and colloidal gold immunochromatographic assay

Immunochromatographic assay (ICA) is an efficient analytical technique and rapid, convenient, easy-to-use, low-cost, and on-site detection method that has been widely used to evaluate food safety. However, an important issue to be addressed for this method is its matrix effect. In this work, time-re...

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Auteurs principaux:	Yong Zhang, Juan Peng, Ping Guo, Guo-Qiang Li, Kai-Yi Zhang, Xi Lv, Wei-Hua Lai
Format:	article
Langue:	EN
Publié:	Taylor & Francis Group 2018
Sujets:	matrix effects immunochromatographic assay clenbuterol Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Accès en ligne:	https://doaj.org/article/5a0fb8e0eb944edc841ab4a43479282a
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spelling	oai:doaj.org-article:5a0fb8e0eb944edc841ab4a43479282a2021-11-26T11:19:46ZMatrix effect of swine urine on time-resolved fluorescent nanobeads and colloidal gold immunochromatographic assay0954-01051465-344310.1080/09540105.2018.1439456https://doaj.org/article/5a0fb8e0eb944edc841ab4a43479282a2018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2018.1439456https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443Immunochromatographic assay (ICA) is an efficient analytical technique and rapid, convenient, easy-to-use, low-cost, and on-site detection method that has been widely used to evaluate food safety. However, an important issue to be addressed for this method is its matrix effect. In this work, time-resolved fluorescent nanobead ICA (TRFN-ICA) and colloidal gold ICA (CG-ICA) were developed to detect clenbuterol in swine urine. Under optimized working conditions, the limits of detection of TRFN-ICA and CG-ICA were 16 and 68 pg/mL, respectively. The matrix effect on TRFN-ICA and CG-ICA was assessed in 20 swine urine samples. Results indicated that the sensitivity of TRFN-ICA was better than that of CG-ICA, and the detection time of the former was shorter than that of the latter. The matrix effect on TRFN-ICA was more serious than that on CG-ICA.Yong ZhangJuan PengPing GuoGuo-Qiang LiKai-Yi ZhangXi LvWei-Hua LaiTaylor & Francis Grouparticlematrix effectsimmunochromatographic assayclenbuterolAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 711-721 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	matrix effects immunochromatographic assay clenbuterol Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	matrix effects immunochromatographic assay clenbuterol Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Yong Zhang Juan Peng Ping Guo Guo-Qiang Li Kai-Yi Zhang Xi Lv Wei-Hua Lai Matrix effect of swine urine on time-resolved fluorescent nanobeads and colloidal gold immunochromatographic assay
description	Immunochromatographic assay (ICA) is an efficient analytical technique and rapid, convenient, easy-to-use, low-cost, and on-site detection method that has been widely used to evaluate food safety. However, an important issue to be addressed for this method is its matrix effect. In this work, time-resolved fluorescent nanobead ICA (TRFN-ICA) and colloidal gold ICA (CG-ICA) were developed to detect clenbuterol in swine urine. Under optimized working conditions, the limits of detection of TRFN-ICA and CG-ICA were 16 and 68 pg/mL, respectively. The matrix effect on TRFN-ICA and CG-ICA was assessed in 20 swine urine samples. Results indicated that the sensitivity of TRFN-ICA was better than that of CG-ICA, and the detection time of the former was shorter than that of the latter. The matrix effect on TRFN-ICA was more serious than that on CG-ICA.
format	article
author	Yong Zhang Juan Peng Ping Guo Guo-Qiang Li Kai-Yi Zhang Xi Lv Wei-Hua Lai
author_facet	Yong Zhang Juan Peng Ping Guo Guo-Qiang Li Kai-Yi Zhang Xi Lv Wei-Hua Lai
author_sort	Yong Zhang
title	Matrix effect of swine urine on time-resolved fluorescent nanobeads and colloidal gold immunochromatographic assay
title_short	Matrix effect of swine urine on time-resolved fluorescent nanobeads and colloidal gold immunochromatographic assay
title_full	Matrix effect of swine urine on time-resolved fluorescent nanobeads and colloidal gold immunochromatographic assay
title_fullStr	Matrix effect of swine urine on time-resolved fluorescent nanobeads and colloidal gold immunochromatographic assay
title_full_unstemmed	Matrix effect of swine urine on time-resolved fluorescent nanobeads and colloidal gold immunochromatographic assay
title_sort	matrix effect of swine urine on time-resolved fluorescent nanobeads and colloidal gold immunochromatographic assay
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/5a0fb8e0eb944edc841ab4a43479282a
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Matrix effect of swine urine on time-resolved fluorescent nanobeads and colloidal gold immunochromatographic assay

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