Phylogenetic Characteristics of Canine Parvovirus Type 2c Variant Endemic in Shanghai, China

Canine parvovirus type 2 (CPV-2) has spread and mutated globally over the past 40 years. In the present study, 206 samples from dogs suspected of CPV-2 infection were collected from five veterinary clinics in Shanghai city, China. The average positive rate for CPV-2 was detected to be 40.78% using t...

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Autores principales:	Chengqian Liu, Jun Gao, Hong Li, Fengping Sun, Hongyu Liang, Huili Liu, Jianzhong Yi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	canine parvovirus CPV-2 VP2 capsid protein Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/5a102cb9f4c44114a96719c568863a36
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spelling	oai:doaj.org-article:5a102cb9f4c44114a96719c568863a362021-11-25T19:13:50ZPhylogenetic Characteristics of Canine Parvovirus Type 2c Variant Endemic in Shanghai, China10.3390/v131122571999-4915https://doaj.org/article/5a102cb9f4c44114a96719c568863a362021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2257https://doaj.org/toc/1999-4915Canine parvovirus type 2 (CPV-2) has spread and mutated globally over the past 40 years. In the present study, 206 samples from dogs suspected of CPV-2 infection were collected from five veterinary clinics in Shanghai city, China. The average positive rate for CPV-2 was detected to be 40.78% using the PCR method. Using an F81 cell (feline kidney cell) culture, the isolates of three CPV-2c strains were obtained. The near full-length genome sequences of the isolates were determined and submitted to GenBank: CPV-SH2001 (MW650830), CPV-SH2002 (MW811188), and CPV-SH2003 (MW811189). By comparing the amino acid sequences of 12 CPV strains with those of 48 related strains retrieved from GenBank, all of the CPV strains from Shanghai were typed as belonging to a relatively new CPV-2c variant spreading in Asia, with typical amino acid residues (5Gly, 267Tyr, 324Ile, and 370Arg) in the VP2 protein. The divergence time of this new CPV-2c clade was estimated by the phylogenetic tree using the maximum likelihood and RelTime with Dated Tips (RTDT) approaches. Our results indicate that the 426 and 324 VP2 amino acid residues are under strong selection pressure with a posterior probability of 0.966 and 0.943, respectively. Therefore, this study provides insight into the phylogenetic characteristics of the current CPV-2c variant in Shanghai city, China.Chengqian LiuJun GaoHong LiFengping SunHongyu LiangHuili LiuJianzhong YiMDPI AGarticlecanine parvovirusCPV-2VP2 capsid proteinMicrobiologyQR1-502ENViruses, Vol 13, Iss 2257, p 2257 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	canine parvovirus CPV-2 VP2 capsid protein Microbiology QR1-502
spellingShingle	canine parvovirus CPV-2 VP2 capsid protein Microbiology QR1-502 Chengqian Liu Jun Gao Hong Li Fengping Sun Hongyu Liang Huili Liu Jianzhong Yi Phylogenetic Characteristics of Canine Parvovirus Type 2c Variant Endemic in Shanghai, China
description	Canine parvovirus type 2 (CPV-2) has spread and mutated globally over the past 40 years. In the present study, 206 samples from dogs suspected of CPV-2 infection were collected from five veterinary clinics in Shanghai city, China. The average positive rate for CPV-2 was detected to be 40.78% using the PCR method. Using an F81 cell (feline kidney cell) culture, the isolates of three CPV-2c strains were obtained. The near full-length genome sequences of the isolates were determined and submitted to GenBank: CPV-SH2001 (MW650830), CPV-SH2002 (MW811188), and CPV-SH2003 (MW811189). By comparing the amino acid sequences of 12 CPV strains with those of 48 related strains retrieved from GenBank, all of the CPV strains from Shanghai were typed as belonging to a relatively new CPV-2c variant spreading in Asia, with typical amino acid residues (5Gly, 267Tyr, 324Ile, and 370Arg) in the VP2 protein. The divergence time of this new CPV-2c clade was estimated by the phylogenetic tree using the maximum likelihood and RelTime with Dated Tips (RTDT) approaches. Our results indicate that the 426 and 324 VP2 amino acid residues are under strong selection pressure with a posterior probability of 0.966 and 0.943, respectively. Therefore, this study provides insight into the phylogenetic characteristics of the current CPV-2c variant in Shanghai city, China.
format	article
author	Chengqian Liu Jun Gao Hong Li Fengping Sun Hongyu Liang Huili Liu Jianzhong Yi
author_facet	Chengqian Liu Jun Gao Hong Li Fengping Sun Hongyu Liang Huili Liu Jianzhong Yi
author_sort	Chengqian Liu
title	Phylogenetic Characteristics of Canine Parvovirus Type 2c Variant Endemic in Shanghai, China
title_short	Phylogenetic Characteristics of Canine Parvovirus Type 2c Variant Endemic in Shanghai, China
title_full	Phylogenetic Characteristics of Canine Parvovirus Type 2c Variant Endemic in Shanghai, China
title_fullStr	Phylogenetic Characteristics of Canine Parvovirus Type 2c Variant Endemic in Shanghai, China
title_full_unstemmed	Phylogenetic Characteristics of Canine Parvovirus Type 2c Variant Endemic in Shanghai, China
title_sort	phylogenetic characteristics of canine parvovirus type 2c variant endemic in shanghai, china
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5a102cb9f4c44114a96719c568863a36
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_version_	1718410157513244672

Phylogenetic Characteristics of Canine Parvovirus Type 2c Variant Endemic in Shanghai, China

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