A new method for the study of biophysical and morphological parameters in 3D cell cultures: Evaluation in LoVo spheroids treated with crizotinib.
Three-dimensional (3D) culture systems like tumor spheroids represent useful in vitro models for drug screening and more broadly for cancer biology research, but the generation of uniform populations of spheroids remains challenging. The possibility to properly characterize spheroid properties would...
Guardado en:
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/5a10e15634274b6788cc361e7c7c8d18 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:5a10e15634274b6788cc361e7c7c8d18 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:5a10e15634274b6788cc361e7c7c8d182021-12-02T20:10:57ZA new method for the study of biophysical and morphological parameters in 3D cell cultures: Evaluation in LoVo spheroids treated with crizotinib.1932-620310.1371/journal.pone.0252907https://doaj.org/article/5a10e15634274b6788cc361e7c7c8d182021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252907https://doaj.org/toc/1932-6203Three-dimensional (3D) culture systems like tumor spheroids represent useful in vitro models for drug screening and more broadly for cancer biology research, but the generation of uniform populations of spheroids remains challenging. The possibility to properly characterize spheroid properties would increase the reliability of these models. To address this issue different analysis were combined: i) a new device and relative analytical method for the accurate, simultaneous, and rapid measurement of mass density, weight, and size of spheroids, ii) confocal imaging, and iii) protein quantification, in a clinically relevant 3D model. The LoVo colon cancer cell line forming spheroids, treated with crizotinib (CZB) an ATP-competitive small-molecule inhibitor of the receptor tyrosine kinases, was employed to study and assess the correlation between biophysical and morphological parameters in both live and fixed cells. The new fluidic-based measurements allowed a robust phenotypical characterization of the spheroids structure, offering insights on the spheroids bulk and an accurate measurement of the tumor density. This analysis helps overcome the technical limits of the imaging that hardly penetrates the thickness of 3D structures. Accordingly, we were able to document that CZB treatment has an impact on mass density, which represents a key marker characterizing cancer cell treatment. Spheroid culture is the ultimate technology in drug discovery and the adoption of such precise measurement of the tumor characteristics can represent a key step forward for the accurate testing of treatment's potential in 3D in vitro models.Azzurra SargentiFrancesco MusmeciCarola CavalloMartina MazzeschiSimone BonettiSimone PasquaFrancesco BacchiGiuseppe FilardoDaniele GazzolaMattia LauriolaSpartaco SantiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0252907 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Azzurra Sargenti Francesco Musmeci Carola Cavallo Martina Mazzeschi Simone Bonetti Simone Pasqua Francesco Bacchi Giuseppe Filardo Daniele Gazzola Mattia Lauriola Spartaco Santi A new method for the study of biophysical and morphological parameters in 3D cell cultures: Evaluation in LoVo spheroids treated with crizotinib. |
| description |
Three-dimensional (3D) culture systems like tumor spheroids represent useful in vitro models for drug screening and more broadly for cancer biology research, but the generation of uniform populations of spheroids remains challenging. The possibility to properly characterize spheroid properties would increase the reliability of these models. To address this issue different analysis were combined: i) a new device and relative analytical method for the accurate, simultaneous, and rapid measurement of mass density, weight, and size of spheroids, ii) confocal imaging, and iii) protein quantification, in a clinically relevant 3D model. The LoVo colon cancer cell line forming spheroids, treated with crizotinib (CZB) an ATP-competitive small-molecule inhibitor of the receptor tyrosine kinases, was employed to study and assess the correlation between biophysical and morphological parameters in both live and fixed cells. The new fluidic-based measurements allowed a robust phenotypical characterization of the spheroids structure, offering insights on the spheroids bulk and an accurate measurement of the tumor density. This analysis helps overcome the technical limits of the imaging that hardly penetrates the thickness of 3D structures. Accordingly, we were able to document that CZB treatment has an impact on mass density, which represents a key marker characterizing cancer cell treatment. Spheroid culture is the ultimate technology in drug discovery and the adoption of such precise measurement of the tumor characteristics can represent a key step forward for the accurate testing of treatment's potential in 3D in vitro models. |
| format |
article |
| author |
Azzurra Sargenti Francesco Musmeci Carola Cavallo Martina Mazzeschi Simone Bonetti Simone Pasqua Francesco Bacchi Giuseppe Filardo Daniele Gazzola Mattia Lauriola Spartaco Santi |
| author_facet |
Azzurra Sargenti Francesco Musmeci Carola Cavallo Martina Mazzeschi Simone Bonetti Simone Pasqua Francesco Bacchi Giuseppe Filardo Daniele Gazzola Mattia Lauriola Spartaco Santi |
| author_sort |
Azzurra Sargenti |
| title |
A new method for the study of biophysical and morphological parameters in 3D cell cultures: Evaluation in LoVo spheroids treated with crizotinib. |
| title_short |
A new method for the study of biophysical and morphological parameters in 3D cell cultures: Evaluation in LoVo spheroids treated with crizotinib. |
| title_full |
A new method for the study of biophysical and morphological parameters in 3D cell cultures: Evaluation in LoVo spheroids treated with crizotinib. |
| title_fullStr |
A new method for the study of biophysical and morphological parameters in 3D cell cultures: Evaluation in LoVo spheroids treated with crizotinib. |
| title_full_unstemmed |
A new method for the study of biophysical and morphological parameters in 3D cell cultures: Evaluation in LoVo spheroids treated with crizotinib. |
| title_sort |
new method for the study of biophysical and morphological parameters in 3d cell cultures: evaluation in lovo spheroids treated with crizotinib. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2021 |
| url |
https://doaj.org/article/5a10e15634274b6788cc361e7c7c8d18 |
| work_keys_str_mv |
AT azzurrasargenti anewmethodforthestudyofbiophysicalandmorphologicalparametersin3dcellculturesevaluationinlovospheroidstreatedwithcrizotinib AT francescomusmeci anewmethodforthestudyofbiophysicalandmorphologicalparametersin3dcellculturesevaluationinlovospheroidstreatedwithcrizotinib AT carolacavallo anewmethodforthestudyofbiophysicalandmorphologicalparametersin3dcellculturesevaluationinlovospheroidstreatedwithcrizotinib AT martinamazzeschi anewmethodforthestudyofbiophysicalandmorphologicalparametersin3dcellculturesevaluationinlovospheroidstreatedwithcrizotinib AT simonebonetti anewmethodforthestudyofbiophysicalandmorphologicalparametersin3dcellculturesevaluationinlovospheroidstreatedwithcrizotinib AT simonepasqua anewmethodforthestudyofbiophysicalandmorphologicalparametersin3dcellculturesevaluationinlovospheroidstreatedwithcrizotinib AT francescobacchi anewmethodforthestudyofbiophysicalandmorphologicalparametersin3dcellculturesevaluationinlovospheroidstreatedwithcrizotinib AT giuseppefilardo anewmethodforthestudyofbiophysicalandmorphologicalparametersin3dcellculturesevaluationinlovospheroidstreatedwithcrizotinib AT danielegazzola anewmethodforthestudyofbiophysicalandmorphologicalparametersin3dcellculturesevaluationinlovospheroidstreatedwithcrizotinib AT mattialauriola anewmethodforthestudyofbiophysicalandmorphologicalparametersin3dcellculturesevaluationinlovospheroidstreatedwithcrizotinib AT spartacosanti anewmethodforthestudyofbiophysicalandmorphologicalparametersin3dcellculturesevaluationinlovospheroidstreatedwithcrizotinib AT azzurrasargenti newmethodforthestudyofbiophysicalandmorphologicalparametersin3dcellculturesevaluationinlovospheroidstreatedwithcrizotinib AT francescomusmeci newmethodforthestudyofbiophysicalandmorphologicalparametersin3dcellculturesevaluationinlovospheroidstreatedwithcrizotinib AT carolacavallo newmethodforthestudyofbiophysicalandmorphologicalparametersin3dcellculturesevaluationinlovospheroidstreatedwithcrizotinib AT martinamazzeschi newmethodforthestudyofbiophysicalandmorphologicalparametersin3dcellculturesevaluationinlovospheroidstreatedwithcrizotinib AT simonebonetti newmethodforthestudyofbiophysicalandmorphologicalparametersin3dcellculturesevaluationinlovospheroidstreatedwithcrizotinib AT simonepasqua newmethodforthestudyofbiophysicalandmorphologicalparametersin3dcellculturesevaluationinlovospheroidstreatedwithcrizotinib AT francescobacchi newmethodforthestudyofbiophysicalandmorphologicalparametersin3dcellculturesevaluationinlovospheroidstreatedwithcrizotinib AT giuseppefilardo newmethodforthestudyofbiophysicalandmorphologicalparametersin3dcellculturesevaluationinlovospheroidstreatedwithcrizotinib AT danielegazzola newmethodforthestudyofbiophysicalandmorphologicalparametersin3dcellculturesevaluationinlovospheroidstreatedwithcrizotinib AT mattialauriola newmethodforthestudyofbiophysicalandmorphologicalparametersin3dcellculturesevaluationinlovospheroidstreatedwithcrizotinib AT spartacosanti newmethodforthestudyofbiophysicalandmorphologicalparametersin3dcellculturesevaluationinlovospheroidstreatedwithcrizotinib |
| _version_ |
1718374947001204736 |